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Ingredients in Zijuan Pu'er Tea Extract Alleviate β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease Likely through DAF-16.
Molecules. 2019 Feb 18; 24(4)M

Abstract

Amyloid-β, one of the hallmarks of Alzheimer's disease (AD), is toxic to neurons and can also cause brain cell death. Oxidative stress is known to play an important role in AD, and there is strong evidence that oxidative stress is associated with amyloid-β. In the present study we report the protective effect of Zijuan Pu'er tea water extract (ZTWE) and the mixture of main ingredients (+)-catechins, caffeine and procyanidin (MCCP) in ZTWE on β-amyloid-induced toxicity in transgenic Caenorhabditis elegans (C. elegans) CL4176 expressing the human Aβ1⁻42 gene. ZTWE, (+)-catechins, caffeine, procyanidin and MCCP delayed the β-amyloid-induced paralysis to different degrees. The MCCP treatment did not affect the transcript abundance of amyloid-β transgene (amy-1); however, Thioflavin T staining showed a significant decrease in Aβ accumulation compared to untreated worms. Further research using transgenic worms found that MCCP promoted the translocation of DAF-16 from cytoplasm to nucleus and increased the expression of superoxide dismutase 3 (SOD-3). In addition, MCCP decreased the reactive oxygen species (ROS) content and increased the SOD activity in CL4176 worms. In conclusion, the results suggested that MCCP had a significant protective effect on β-amyloid-induced toxicity in C. elegans by reducing β-amyloid aggregation and inducing DAF-16 nuclear translocation that could activate the downstream signal pathway and enhance resistance to oxidative stress.

Authors+Show Affiliations

National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China. du_fangzhou@163.com.National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China. 18744021315@163.com.National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China. jiaoyan@novogene.com.National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China. baisj16@mails.jlu.edu.cn.National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China. luwang_0925@163.com.National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China. mjf@jlu.edu.cn. Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. mjf@jlu.edu.cn.National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China. fxq@jlu.edu.cn. Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. fxq@jlu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30781621

Citation

Du, Fangzhou, et al. "Ingredients in Zijuan Pu'er Tea Extract Alleviate β-Amyloid Peptide Toxicity in a Caenorhabditis Elegans Model of Alzheimer's Disease Likely Through DAF-16." Molecules (Basel, Switzerland), vol. 24, no. 4, 2019.
Du F, Zhou L, Jiao Y, et al. Ingredients in Zijuan Pu'er Tea Extract Alleviate β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease Likely through DAF-16. Molecules. 2019;24(4).
Du, F., Zhou, L., Jiao, Y., Bai, S., Wang, L., Ma, J., & Fu, X. (2019). Ingredients in Zijuan Pu'er Tea Extract Alleviate β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease Likely through DAF-16. Molecules (Basel, Switzerland), 24(4). https://doi.org/10.3390/molecules24040729
Du F, et al. Ingredients in Zijuan Pu'er Tea Extract Alleviate β-Amyloid Peptide Toxicity in a Caenorhabditis Elegans Model of Alzheimer's Disease Likely Through DAF-16. Molecules. 2019 Feb 18;24(4) PubMed PMID: 30781621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ingredients in Zijuan Pu'er Tea Extract Alleviate β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease Likely through DAF-16. AU - Du,Fangzhou, AU - Zhou,Lin, AU - Jiao,Yan, AU - Bai,Shuju, AU - Wang,Lu, AU - Ma,Junfeng, AU - Fu,Xueqi, Y1 - 2019/02/18/ PY - 2018/12/24/received PY - 2019/02/01/revised PY - 2019/02/04/accepted PY - 2019/2/21/entrez PY - 2019/2/20/pubmed PY - 2020/1/3/medline KW - Alzheimer’s disease KW - C. elegans KW - amyloid-β KW - oxidative stress JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 4 N2 - Amyloid-β, one of the hallmarks of Alzheimer's disease (AD), is toxic to neurons and can also cause brain cell death. Oxidative stress is known to play an important role in AD, and there is strong evidence that oxidative stress is associated with amyloid-β. In the present study we report the protective effect of Zijuan Pu'er tea water extract (ZTWE) and the mixture of main ingredients (+)-catechins, caffeine and procyanidin (MCCP) in ZTWE on β-amyloid-induced toxicity in transgenic Caenorhabditis elegans (C. elegans) CL4176 expressing the human Aβ1⁻42 gene. ZTWE, (+)-catechins, caffeine, procyanidin and MCCP delayed the β-amyloid-induced paralysis to different degrees. The MCCP treatment did not affect the transcript abundance of amyloid-β transgene (amy-1); however, Thioflavin T staining showed a significant decrease in Aβ accumulation compared to untreated worms. Further research using transgenic worms found that MCCP promoted the translocation of DAF-16 from cytoplasm to nucleus and increased the expression of superoxide dismutase 3 (SOD-3). In addition, MCCP decreased the reactive oxygen species (ROS) content and increased the SOD activity in CL4176 worms. In conclusion, the results suggested that MCCP had a significant protective effect on β-amyloid-induced toxicity in C. elegans by reducing β-amyloid aggregation and inducing DAF-16 nuclear translocation that could activate the downstream signal pathway and enhance resistance to oxidative stress. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30781621/Ingredients_in_Zijuan_Pu'er_Tea_Extract_Alleviate_β_Amyloid_Peptide_Toxicity_in_a_Caenorhabditis_elegans_Model_of_Alzheimer's_Disease_Likely_through_DAF_16_ L2 - http://www.mdpi.com/resolver?pii=molecules24040729 DB - PRIME DP - Unbound Medicine ER -