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Hydrogen peroxide-induced oxidative stress upregulates ght5 gene belonging to hexose transporters in Schizosaccharomyces pombe.
Cell Mol Biol (Noisy-le-grand). 2019 Jan 31; 65(1):41-45.CM

Abstract

Hydrogen peroxide is an agent that triggers oxidative stress. Glucose, which is a source of carbon and energy has a regulatory role in many metabolic processes such as growth rate, fermentation capacity and stress response. Schizosaccharomyces pombe has eight hexose transporters with a different affinity for glucose and/or related monosaccharides. In S. pombe, Ght5 is a glucose transporter with high-affinity. We aimed to investigate the effects of H2O2-induced oxidative stress on hexose transporters using glucose repression-resistant mutant strains (ird5 and ird11) of S. pombe. We analyzed the percentage of glucose consumption in S. pombe wild-type and mutant cells under stressed and non-stressed conditions. Then we compared the expression levels of the genes encoding hexose transporters under the same conditions. We confirmed that the glucose consumption efficiencies of the mutants were slower than the wild-type as in earlier study under non-stressed condition. The percentage of glucose consumption reduced by approximately two-fold in ird11 and wild-type, but not change in ird5, under a stressed condition. There is no difference between cells shape and size of S. pombe strains under stressed and non-stressed conditions. Under stress-induced condition, the expression levels of ght3, ght4 genes in ird11 and wild-type, and ght4, ght6 genes in ird5 decreased, but that of ght5 gene remarkably increased in only wild-type. We suggested that oxidative stress caused by H2O2 leads to upregulation of the ght5 gene in S. pombe.

Authors+Show Affiliations

Department of Molecular Biology and Genetics, Institute of Life Sciences and Biotechnology, Bezmialem Vakıf University, İstanbul, Turkey.Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30782292

Citation

Kına, Umit Yasar, and Bedia Palabiyik. "Hydrogen Peroxide-induced Oxidative Stress Upregulates Ght5 Gene Belonging to Hexose Transporters in Schizosaccharomyces Pombe." Cellular and Molecular Biology (Noisy-le-Grand, France), vol. 65, no. 1, 2019, pp. 41-45.
Kına UY, Palabiyik B. Hydrogen peroxide-induced oxidative stress upregulates ght5 gene belonging to hexose transporters in Schizosaccharomyces pombe. Cell Mol Biol (Noisy-le-grand). 2019;65(1):41-45.
Kına, U. Y., & Palabiyik, B. (2019). Hydrogen peroxide-induced oxidative stress upregulates ght5 gene belonging to hexose transporters in Schizosaccharomyces pombe. Cellular and Molecular Biology (Noisy-le-Grand, France), 65(1), 41-45.
Kına UY, Palabiyik B. Hydrogen Peroxide-induced Oxidative Stress Upregulates Ght5 Gene Belonging to Hexose Transporters in Schizosaccharomyces Pombe. Cell Mol Biol (Noisy-le-grand). 2019 Jan 31;65(1):41-45. PubMed PMID: 30782292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen peroxide-induced oxidative stress upregulates ght5 gene belonging to hexose transporters in Schizosaccharomyces pombe. AU - Kına,Umit Yasar, AU - Palabiyik,Bedia, Y1 - 2019/01/31/ PY - 2018/10/08/received PY - 2018/11/30/accepted PY - 2019/01/07/revised PY - 2019/2/21/entrez PY - 2019/2/21/pubmed PY - 2019/5/7/medline KW - Glucose repression. KW - Hexose transporters KW - Oxidative stress KW - Schizosaccharomyces pombe KW - ght5 SP - 41 EP - 45 JF - Cellular and molecular biology (Noisy-le-Grand, France) JO - Cell Mol Biol (Noisy-le-grand) VL - 65 IS - 1 N2 - Hydrogen peroxide is an agent that triggers oxidative stress. Glucose, which is a source of carbon and energy has a regulatory role in many metabolic processes such as growth rate, fermentation capacity and stress response. Schizosaccharomyces pombe has eight hexose transporters with a different affinity for glucose and/or related monosaccharides. In S. pombe, Ght5 is a glucose transporter with high-affinity. We aimed to investigate the effects of H2O2-induced oxidative stress on hexose transporters using glucose repression-resistant mutant strains (ird5 and ird11) of S. pombe. We analyzed the percentage of glucose consumption in S. pombe wild-type and mutant cells under stressed and non-stressed conditions. Then we compared the expression levels of the genes encoding hexose transporters under the same conditions. We confirmed that the glucose consumption efficiencies of the mutants were slower than the wild-type as in earlier study under non-stressed condition. The percentage of glucose consumption reduced by approximately two-fold in ird11 and wild-type, but not change in ird5, under a stressed condition. There is no difference between cells shape and size of S. pombe strains under stressed and non-stressed conditions. Under stress-induced condition, the expression levels of ght3, ght4 genes in ird11 and wild-type, and ght4, ght6 genes in ird5 decreased, but that of ght5 gene remarkably increased in only wild-type. We suggested that oxidative stress caused by H2O2 leads to upregulation of the ght5 gene in S. pombe. SN - 1165-158X UR - https://www.unboundmedicine.com/medline/citation/30782292/Hydrogen_peroxide_induced_oxidative_stress_upregulates_ght5_gene_belonging_to_hexose_transporters_in_Schizosaccharomyces_pombe_ DB - PRIME DP - Unbound Medicine ER -