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Lateral Flow Aptasensor for Simultaneous Detection of Platelet-Derived Growth Factor-BB (PDGF-BB) and Thrombin.
Molecules 2019; 24(4)M

Abstract

Here we report a lateral flow aptasensor (LFA) for the simultaneous detection of platelet-derived growth factor-BB (PDGF-BB) and thrombin. Two pairs of aptamers, which are specific against PDGF-BB and thrombin, respectively, were used to prepare the LFA. Thiolated aptamers were immobilized on a gold nanoparticle (AuNP) surface and biotinylated aptamers were immobilized on the test zones of an LFA nitrocellulose membrane. The assay involved the capture of PDGF-BB and thrombin simultaneously in sandwich-type formats between the capture aptamers on the test zones of LFA and AuNP-labeled detection aptamers. AuNPs were thus captured on the test zones of the LFA and gave red bands to enable the visual detection of target proteins. Quantitative results were obtained by reading the test band intensities with a portable strip reader. By combining the highly specific molecular recognition properties of aptamers with the unique properties of lateral flow assay (low-cost, short assay time and a user-friendly format), the optimized aptasensor was capable of simultaneously detecting 1.0 nM of PDGF-BB and 1.5 nM of thrombin in association with a 10-min assay time. The biosensor was also successfully applied to detect PDGF-BB and thrombin in spiked human serum samples. The LFA shows great promise for the development of aptamer-based lateral flow strip biosensors for point-of-care or for the in-field detection of disease-related protein biomarkers.

Authors+Show Affiliations

Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Chuzhou 233100, China. guodong.liu@ndsu.edu. Department of Chemistry & Biochemistry, North Dakota State University, Fargo, ND 58105, USA. guodong.liu@ndsu.edu.Department of Chemistry & Biochemistry, North Dakota State University, Fargo, ND 58105, USA. anant.gurung@ndsu.edu.Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Chuzhou 233100, China. qiuwanweiqww@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30791526

Citation

Liu, Guodong, et al. "Lateral Flow Aptasensor for Simultaneous Detection of Platelet-Derived Growth Factor-BB (PDGF-BB) and Thrombin." Molecules (Basel, Switzerland), vol. 24, no. 4, 2019.
Liu G, Gurung AS, Qiu W. Lateral Flow Aptasensor for Simultaneous Detection of Platelet-Derived Growth Factor-BB (PDGF-BB) and Thrombin. Molecules. 2019;24(4).
Liu, G., Gurung, A. S., & Qiu, W. (2019). Lateral Flow Aptasensor for Simultaneous Detection of Platelet-Derived Growth Factor-BB (PDGF-BB) and Thrombin. Molecules (Basel, Switzerland), 24(4), doi:10.3390/molecules24040756.
Liu G, Gurung AS, Qiu W. Lateral Flow Aptasensor for Simultaneous Detection of Platelet-Derived Growth Factor-BB (PDGF-BB) and Thrombin. Molecules. 2019 Feb 20;24(4) PubMed PMID: 30791526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lateral Flow Aptasensor for Simultaneous Detection of Platelet-Derived Growth Factor-BB (PDGF-BB) and Thrombin. AU - Liu,Guodong, AU - Gurung,Anant S, AU - Qiu,Wanwei, Y1 - 2019/02/20/ PY - 2019/01/13/received PY - 2019/02/01/revised PY - 2019/02/02/accepted PY - 2019/2/23/entrez PY - 2019/2/23/pubmed PY - 2019/6/5/medline KW - PDGF-BB KW - aptamer KW - aptasensor KW - lateral flow KW - thrombin JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 4 N2 - Here we report a lateral flow aptasensor (LFA) for the simultaneous detection of platelet-derived growth factor-BB (PDGF-BB) and thrombin. Two pairs of aptamers, which are specific against PDGF-BB and thrombin, respectively, were used to prepare the LFA. Thiolated aptamers were immobilized on a gold nanoparticle (AuNP) surface and biotinylated aptamers were immobilized on the test zones of an LFA nitrocellulose membrane. The assay involved the capture of PDGF-BB and thrombin simultaneously in sandwich-type formats between the capture aptamers on the test zones of LFA and AuNP-labeled detection aptamers. AuNPs were thus captured on the test zones of the LFA and gave red bands to enable the visual detection of target proteins. Quantitative results were obtained by reading the test band intensities with a portable strip reader. By combining the highly specific molecular recognition properties of aptamers with the unique properties of lateral flow assay (low-cost, short assay time and a user-friendly format), the optimized aptasensor was capable of simultaneously detecting 1.0 nM of PDGF-BB and 1.5 nM of thrombin in association with a 10-min assay time. The biosensor was also successfully applied to detect PDGF-BB and thrombin in spiked human serum samples. The LFA shows great promise for the development of aptamer-based lateral flow strip biosensors for point-of-care or for the in-field detection of disease-related protein biomarkers. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30791526/Lateral_Flow_Aptasensor_for_Simultaneous_Detection_of_Platelet-Derived_Growth_Factor-BB_(PDGF-BB)_and_Thrombin L2 - http://www.mdpi.com/resolver?pii=molecules24040756 DB - PRIME DP - Unbound Medicine ER -