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The proteolytic effect of mast cell tryptase to eotaxin-1/CCL11·eotaxin-2/CCL24 and eotaxin-3/CCL26 produced by conjunctival fibroblasts.
Jpn J Ophthalmol 2019; 63(2):215-220JJ

Abstract

PURPOSE

To investigate the proteolytic effect of mast cell tryptase on eotaxin-1/CCL11, eotaxin-2/CCL24 and eotaxin-3/CCL26 produced by conjunctival fibroblasts.

STUDY DESIGN

Experimental.

METHODS

The production of eotaxin-1, -2 and -3 by conjunctival fibroblasts stimulated both with and without IL-4/IL-13 or/and TGF-β1 was assessed by ELISA. The proteolytic activity of tryptase on eotaxins derived from conjunctival fibroblasts and recombinant eotaxins was also estimated by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR).

RESULTS

Conjunctival fibroblasts produced eotaxin-1 and -3, but not eotaxin-2. Stimulation with IL-4/IL-13 and TGF-β1 synergistically increased eotaxin-1 and -3 production. Tryptase reduced the immunoreactivity of eotaxin-1 and -3 but not of eotaxin-2, due to the proteolysis of these eotaxins but not the inhibition of their m-RNA expression.

CONCLUSION

Mast cell tryptase may exercise proteolytic activity on eotaxin-1 and -3 produced by conjunctival fibroblasts, resulting in partial suppression of the ability of eotaxin-1 and -3 to accumulate eosinophils in the conjunctiva. Eotaxin-2 in the tears may be a suitable biomarker of severity of allergic conjunctival disease.

Authors+Show Affiliations

Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.Department of Ophthalmology, Juntendo University Urayasu Hospital, 2-1-1, Tomioka Urayasu-shi, Chiba, 279-0021, Japan. ebihara@juntendo.ac.jp. Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Chiba, Japan. ebihara@juntendo.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30796548

Citation

Miyagawa, Yukiko, et al. "The Proteolytic Effect of Mast Cell Tryptase to eotaxin-1/CCL11·eotaxin-2/CCL24 and eotaxin-3/CCL26 Produced By Conjunctival Fibroblasts." Japanese Journal of Ophthalmology, vol. 63, no. 2, 2019, pp. 215-220.
Miyagawa Y, Murakami A, Ebihara N. The proteolytic effect of mast cell tryptase to eotaxin-1/CCL11·eotaxin-2/CCL24 and eotaxin-3/CCL26 produced by conjunctival fibroblasts. Jpn J Ophthalmol. 2019;63(2):215-220.
Miyagawa, Y., Murakami, A., & Ebihara, N. (2019). The proteolytic effect of mast cell tryptase to eotaxin-1/CCL11·eotaxin-2/CCL24 and eotaxin-3/CCL26 produced by conjunctival fibroblasts. Japanese Journal of Ophthalmology, 63(2), pp. 215-220. doi:10.1007/s10384-019-00655-w.
Miyagawa Y, Murakami A, Ebihara N. The Proteolytic Effect of Mast Cell Tryptase to eotaxin-1/CCL11·eotaxin-2/CCL24 and eotaxin-3/CCL26 Produced By Conjunctival Fibroblasts. Jpn J Ophthalmol. 2019;63(2):215-220. PubMed PMID: 30796548.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The proteolytic effect of mast cell tryptase to eotaxin-1/CCL11·eotaxin-2/CCL24 and eotaxin-3/CCL26 produced by conjunctival fibroblasts. AU - Miyagawa,Yukiko, AU - Murakami,Akira, AU - Ebihara,Nobuyuki, Y1 - 2019/02/22/ PY - 2018/06/01/received PY - 2018/12/25/accepted PY - 2019/2/24/pubmed PY - 2019/3/28/medline PY - 2019/2/24/entrez KW - Biomarker KW - Eotaxins KW - Tryptase KW - Vernal Keratoconjunctivitis SP - 215 EP - 220 JF - Japanese journal of ophthalmology JO - Jpn. J. Ophthalmol. VL - 63 IS - 2 N2 - PURPOSE: To investigate the proteolytic effect of mast cell tryptase on eotaxin-1/CCL11, eotaxin-2/CCL24 and eotaxin-3/CCL26 produced by conjunctival fibroblasts. STUDY DESIGN: Experimental. METHODS: The production of eotaxin-1, -2 and -3 by conjunctival fibroblasts stimulated both with and without IL-4/IL-13 or/and TGF-β1 was assessed by ELISA. The proteolytic activity of tryptase on eotaxins derived from conjunctival fibroblasts and recombinant eotaxins was also estimated by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). RESULTS: Conjunctival fibroblasts produced eotaxin-1 and -3, but not eotaxin-2. Stimulation with IL-4/IL-13 and TGF-β1 synergistically increased eotaxin-1 and -3 production. Tryptase reduced the immunoreactivity of eotaxin-1 and -3 but not of eotaxin-2, due to the proteolysis of these eotaxins but not the inhibition of their m-RNA expression. CONCLUSION: Mast cell tryptase may exercise proteolytic activity on eotaxin-1 and -3 produced by conjunctival fibroblasts, resulting in partial suppression of the ability of eotaxin-1 and -3 to accumulate eosinophils in the conjunctiva. Eotaxin-2 in the tears may be a suitable biomarker of severity of allergic conjunctival disease. SN - 1613-2246 UR - https://www.unboundmedicine.com/medline/citation/30796548/The_proteolytic_effect_of_mast_cell_tryptase_to_eotaxin-1/CCL11·eotaxin-2/CCL24_and_eotaxin-3/CCL26_produced_by_conjunctival_fibroblasts L2 - https://dx.doi.org/10.1007/s10384-019-00655-w DB - PRIME DP - Unbound Medicine ER -