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Anti-oxidant and anti-inflammatory effects of cinnamaldehyde and eugenol on mononuclear cells of rheumatoid arthritis patients.
Eur J Pharmacol. 2019 Jun 05; 852:14-24.EJ

Abstract

Rheumatoid arthritis (RA) is an autoimmune disorder affecting joints and frequently characterized by initial local and later systemic inflammation. The present study was conducted with the aim to determine the anti-inflammatory and antioxidant effects of cinnamaldehyde and eugenol in the peripheral blood mononuclear cells (PBMC) of RA patients. PBMCs obtained from RA patients were treated with varying concentrations of cinnamaldehyde and eugenol. The levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were monitored in the 24-h culture supernatant of PBMCs. Reactive oxygen species formation, biomolecular oxidation and the activities of antioxidant enzymes were also determined. FTIR analysis was done to determine structural alterations in the PBMCs. Molecular docking was performed to gain an insight into the binding mechanism of eugenol and cinnamaldehyde with pro-inflammatory cytokines. The levels of pro-inflammatory cytokines and markers of oxidative stress were found to be elevated in the PBMC culture of RA patients as compared to the healthy controls. Cinnamaldehyde and eugenol have significantly reduced the levels of cytokines. Reactive oxygen species formation, biomolecular oxidation and antioxidant defense response were also ameliorated by treating PBMCs with both the compounds. FTIR results further confirms cinnamaldehyde and eugenol mediated protection to biomolecules of PBMCs of RA patients. Molecular docking results indicates interaction of cinnamaldehyde and eugenol with key residues of TNF-α and IL-6. Cinnamaldehyde and eugenol were found to exert potent anti-inflammatory and anti-oxidant effects on the PBMC culture of RA patients. So, these compounds may be used as an adjunct in the management of RA.

Authors+Show Affiliations

Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: mrehman@ksu.edu.sa.Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.Department of Pharmacology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.Department of Orthopaedic Surgery, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: moskhan@ksu.edu.sa.Department of Food Science and Nutrition, College of Food and Agriculture, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: fhussain@ksu.edu.sa.Department of Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India. Electronic address: smateen@myamu.ac.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30796902

Citation

Mateen, Somaiya, et al. "Anti-oxidant and Anti-inflammatory Effects of Cinnamaldehyde and Eugenol On Mononuclear Cells of Rheumatoid Arthritis Patients." European Journal of Pharmacology, vol. 852, 2019, pp. 14-24.
Mateen S, Rehman MT, Shahzad S, et al. Anti-oxidant and anti-inflammatory effects of cinnamaldehyde and eugenol on mononuclear cells of rheumatoid arthritis patients. Eur J Pharmacol. 2019;852:14-24.
Mateen, S., Rehman, M. T., Shahzad, S., Naeem, S. S., Faizy, A. F., Khan, A. Q., Khan, M. S., Husain, F. M., & Moin, S. (2019). Anti-oxidant and anti-inflammatory effects of cinnamaldehyde and eugenol on mononuclear cells of rheumatoid arthritis patients. European Journal of Pharmacology, 852, 14-24. https://doi.org/10.1016/j.ejphar.2019.02.031
Mateen S, et al. Anti-oxidant and Anti-inflammatory Effects of Cinnamaldehyde and Eugenol On Mononuclear Cells of Rheumatoid Arthritis Patients. Eur J Pharmacol. 2019 Jun 5;852:14-24. PubMed PMID: 30796902.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-oxidant and anti-inflammatory effects of cinnamaldehyde and eugenol on mononuclear cells of rheumatoid arthritis patients. AU - Mateen,Somaiya, AU - Rehman,Md Tabish, AU - Shahzad,Sumayya, AU - Naeem,Syed Shariq, AU - Faizy,Abul Faiz, AU - Khan,Abdul Qayyum, AU - Khan,Mohd Shahnawaz, AU - Husain,Fohad Mabood, AU - Moin,Shagufta, Y1 - 2019/02/20/ PY - 2018/11/21/received PY - 2019/02/16/revised PY - 2019/02/20/accepted PY - 2019/2/24/pubmed PY - 2019/9/4/medline PY - 2019/2/24/entrez KW - Cinnamaldehyde KW - Eugenol KW - Inflammation KW - Oxidative stress KW - Rheumatoid arthritis SP - 14 EP - 24 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 852 N2 - Rheumatoid arthritis (RA) is an autoimmune disorder affecting joints and frequently characterized by initial local and later systemic inflammation. The present study was conducted with the aim to determine the anti-inflammatory and antioxidant effects of cinnamaldehyde and eugenol in the peripheral blood mononuclear cells (PBMC) of RA patients. PBMCs obtained from RA patients were treated with varying concentrations of cinnamaldehyde and eugenol. The levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were monitored in the 24-h culture supernatant of PBMCs. Reactive oxygen species formation, biomolecular oxidation and the activities of antioxidant enzymes were also determined. FTIR analysis was done to determine structural alterations in the PBMCs. Molecular docking was performed to gain an insight into the binding mechanism of eugenol and cinnamaldehyde with pro-inflammatory cytokines. The levels of pro-inflammatory cytokines and markers of oxidative stress were found to be elevated in the PBMC culture of RA patients as compared to the healthy controls. Cinnamaldehyde and eugenol have significantly reduced the levels of cytokines. Reactive oxygen species formation, biomolecular oxidation and antioxidant defense response were also ameliorated by treating PBMCs with both the compounds. FTIR results further confirms cinnamaldehyde and eugenol mediated protection to biomolecules of PBMCs of RA patients. Molecular docking results indicates interaction of cinnamaldehyde and eugenol with key residues of TNF-α and IL-6. Cinnamaldehyde and eugenol were found to exert potent anti-inflammatory and anti-oxidant effects on the PBMC culture of RA patients. So, these compounds may be used as an adjunct in the management of RA. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/30796902/Anti_oxidant_and_anti_inflammatory_effects_of_cinnamaldehyde_and_eugenol_on_mononuclear_cells_of_rheumatoid_arthritis_patients_ DB - PRIME DP - Unbound Medicine ER -