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Interplay between CTHRC1 and the SU protein of avian leukosis virus subgroup J (ALV-J) facilitates viral replication.
Virus Res. 2019 04 15; 264:32-39.VR

Abstract

The lifecycle of avian leukosis virus subgroup J (ALV-J), a typical tumorigenic retrovirus, is highly dependent upon host cellular proteins. However, there have been few studies directed at uncovering the host proteins responsible for ALV-J replication, which could provide insights into new strategies for ALV-J prevention and control. Here, we used proteomics to identify the association of differential levels of collagen triple helix-repeat-containing 1 (CTHRC1) and with viral replication. Our results revealed that CTHRC1 was significantly upregulated in ALV-J-infected cells in vitro, and these findings were confirmed in vivo. Additionally, CTHRC1 overexpression facilitated ALV-J replication, whereas CTHRC1 knockdown suppressed this activity. Moreover, we found that ALV-J drove CTHRC1 translocation from the nucleus to the cytosol through interactions with the ALV-J envelope glycoprotein. These results revealed CTHRC1 as a shutting protein is recruited by ALV-J to facilitate viral replication.

Authors+Show Affiliations

College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China. Electronic address: park_pang@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China. Electronic address: zhoudefang@126.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China. Electronic address: xuejingwen19941213@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China. Electronic address: tiramisurd@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China. Electronic address: m17661212469@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China. Electronic address: zgyazad@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China. Electronic address: shiyuyuan@163.com.The Pirbright Institute & UK-China Centre of Excellence on Avian Disease Research, Pirbright, Ash Road, Guildford, Surrey, GU24 0NF, UK. Electronic address: yongxiu.yao@pirbright.ac.uk.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China. Electronic address: czqsd@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30797826

Citation

Pang, Yu, et al. "Interplay Between CTHRC1 and the SU Protein of Avian Leukosis Virus Subgroup J (ALV-J) Facilitates Viral Replication." Virus Research, vol. 264, 2019, pp. 32-39.
Pang Y, Zhou D, Xue J, et al. Interplay between CTHRC1 and the SU protein of avian leukosis virus subgroup J (ALV-J) facilitates viral replication. Virus Res. 2019;264:32-39.
Pang, Y., Zhou, D., Xue, J., Zhou, J., Zhang, Y., Zheng, G., Yuan, S., Yao, Y., & Cheng, Z. (2019). Interplay between CTHRC1 and the SU protein of avian leukosis virus subgroup J (ALV-J) facilitates viral replication. Virus Research, 264, 32-39. https://doi.org/10.1016/j.virusres.2019.02.014
Pang Y, et al. Interplay Between CTHRC1 and the SU Protein of Avian Leukosis Virus Subgroup J (ALV-J) Facilitates Viral Replication. Virus Res. 2019 04 15;264:32-39. PubMed PMID: 30797826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interplay between CTHRC1 and the SU protein of avian leukosis virus subgroup J (ALV-J) facilitates viral replication. AU - Pang,Yu, AU - Zhou,Defang, AU - Xue,Jingwen, AU - Zhou,Jing, AU - Zhang,Ya, AU - Zheng,Gaoying, AU - Yuan,Shiyu, AU - Yao,Yongxiu, AU - Cheng,Ziqiang, Y1 - 2019/02/21/ PY - 2019/01/08/received PY - 2019/02/20/revised PY - 2019/02/20/accepted PY - 2019/2/25/pubmed PY - 2019/6/4/medline PY - 2019/2/25/entrez KW - ALV-J KW - CTHRC1 KW - Proteomics KW - Viral replication SP - 32 EP - 39 JF - Virus research JO - Virus Res. VL - 264 N2 - The lifecycle of avian leukosis virus subgroup J (ALV-J), a typical tumorigenic retrovirus, is highly dependent upon host cellular proteins. However, there have been few studies directed at uncovering the host proteins responsible for ALV-J replication, which could provide insights into new strategies for ALV-J prevention and control. Here, we used proteomics to identify the association of differential levels of collagen triple helix-repeat-containing 1 (CTHRC1) and with viral replication. Our results revealed that CTHRC1 was significantly upregulated in ALV-J-infected cells in vitro, and these findings were confirmed in vivo. Additionally, CTHRC1 overexpression facilitated ALV-J replication, whereas CTHRC1 knockdown suppressed this activity. Moreover, we found that ALV-J drove CTHRC1 translocation from the nucleus to the cytosol through interactions with the ALV-J envelope glycoprotein. These results revealed CTHRC1 as a shutting protein is recruited by ALV-J to facilitate viral replication. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/30797826/Interplay_between_CTHRC1_and_the_SU_protein_of_avian_leukosis_virus_subgroup_J__ALV_J__facilitates_viral_replication_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(19)30026-7 DB - PRIME DP - Unbound Medicine ER -