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Staphylococcus aureus related to bovine mastitis in Switzerland: Clonal diversity, virulence gene profiles, and antimicrobial resistance of isolates collected throughout 2017.
J Dairy Sci. 2019 Apr; 102(4):3274-3281.JD

Abstract

Staphylococcus aureus can be associated with subclinical, acute, chronic, and toxic cases of bovine intramammary infections, leading to considerable financial losses for the dairy industry in Switzerland and worldwide. In addition, milk products are one of the most common food categories implicated in staphylococcal food poisoning in humans. Detailed information on the population structure, as well as the virulence and resistance characteristics of Staph. aureus originating from bovine mastitis milk is needed to allow for source attribution and risk assessment of Staph. aureus in a food poisoning context and to improve therapeutic approaches in cattle. Our objective was to assess the population structure, phenotypic resistance patterns, and virulence and resistance gene profiles of Staph. aureus isolates from bovine mastitis milk in Switzerland. To this end, 58 Staph. aureus strains were characterized. The DNA microarray was used to test for the presence or absence of virulence and resistance genes. In addition, minimum inhibitory concentrations of various antimicrobial agents were determined by microdilution. To assess the population structure of the isolates, we determined clonal complexes (CC) using DNA microarray hybridization profiles and performed multilocus sequence typing and spa typing. The strains were assigned to 7 clonal complexes, 10 sequence types, and 11 spa types, with CC705 (43%), CC97 (33%), and CC20 (12%) representing the most common lineages and t529 (43%) and t267 (21%) representing the most common spa types. Only 1 isolate was assigned to CC8, a clonal lineage linked to high within-herd prevalence of mastitis. A total of 14% (n = 8) of strains were classified as resistant to penicillin, and 1 strain each was classified as oxacillin and pirlimycin resistant. Although no clinical breakpoints are available for the combination of kanamycin/cefalexin, growth of all strains was inhibited by the lowest combination of kanamycin/cefalexin concentrations tested (4 µg/mL of kanamycin and 0.4 µg/mL of cefalexin). One strain assigned to CC20, ST389, and t2094 exhibited resistance to penicillin, oxacillin, and pirlimycin as well as intermediate susceptibility to erythromycin and high minimum inhibitory concentration for several antimicrobial agents, for which no breakpoints were available.

Authors+Show Affiliations

Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland. Electronic address: sophia.johler@uzh.ch.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30799111

Citation

Käppeli, N, et al. "Staphylococcus Aureus Related to Bovine Mastitis in Switzerland: Clonal Diversity, Virulence Gene Profiles, and Antimicrobial Resistance of Isolates Collected Throughout 2017." Journal of Dairy Science, vol. 102, no. 4, 2019, pp. 3274-3281.
Käppeli N, Morach M, Corti S, et al. Staphylococcus aureus related to bovine mastitis in Switzerland: Clonal diversity, virulence gene profiles, and antimicrobial resistance of isolates collected throughout 2017. J Dairy Sci. 2019;102(4):3274-3281.
Käppeli, N., Morach, M., Corti, S., Eicher, C., Stephan, R., & Johler, S. (2019). Staphylococcus aureus related to bovine mastitis in Switzerland: Clonal diversity, virulence gene profiles, and antimicrobial resistance of isolates collected throughout 2017. Journal of Dairy Science, 102(4), 3274-3281. https://doi.org/10.3168/jds.2018-15317
Käppeli N, et al. Staphylococcus Aureus Related to Bovine Mastitis in Switzerland: Clonal Diversity, Virulence Gene Profiles, and Antimicrobial Resistance of Isolates Collected Throughout 2017. J Dairy Sci. 2019;102(4):3274-3281. PubMed PMID: 30799111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Staphylococcus aureus related to bovine mastitis in Switzerland: Clonal diversity, virulence gene profiles, and antimicrobial resistance of isolates collected throughout 2017. AU - Käppeli,N, AU - Morach,M, AU - Corti,S, AU - Eicher,C, AU - Stephan,R, AU - Johler,S, Y1 - 2019/02/22/ PY - 2018/07/02/received PY - 2018/12/23/accepted PY - 2019/2/26/pubmed PY - 2019/4/24/medline PY - 2019/2/26/entrez KW - Staphylococcus aureus KW - bovine mastitis KW - minimum inhibitory concentration KW - population structure SP - 3274 EP - 3281 JF - Journal of dairy science JO - J Dairy Sci VL - 102 IS - 4 N2 - Staphylococcus aureus can be associated with subclinical, acute, chronic, and toxic cases of bovine intramammary infections, leading to considerable financial losses for the dairy industry in Switzerland and worldwide. In addition, milk products are one of the most common food categories implicated in staphylococcal food poisoning in humans. Detailed information on the population structure, as well as the virulence and resistance characteristics of Staph. aureus originating from bovine mastitis milk is needed to allow for source attribution and risk assessment of Staph. aureus in a food poisoning context and to improve therapeutic approaches in cattle. Our objective was to assess the population structure, phenotypic resistance patterns, and virulence and resistance gene profiles of Staph. aureus isolates from bovine mastitis milk in Switzerland. To this end, 58 Staph. aureus strains were characterized. The DNA microarray was used to test for the presence or absence of virulence and resistance genes. In addition, minimum inhibitory concentrations of various antimicrobial agents were determined by microdilution. To assess the population structure of the isolates, we determined clonal complexes (CC) using DNA microarray hybridization profiles and performed multilocus sequence typing and spa typing. The strains were assigned to 7 clonal complexes, 10 sequence types, and 11 spa types, with CC705 (43%), CC97 (33%), and CC20 (12%) representing the most common lineages and t529 (43%) and t267 (21%) representing the most common spa types. Only 1 isolate was assigned to CC8, a clonal lineage linked to high within-herd prevalence of mastitis. A total of 14% (n = 8) of strains were classified as resistant to penicillin, and 1 strain each was classified as oxacillin and pirlimycin resistant. Although no clinical breakpoints are available for the combination of kanamycin/cefalexin, growth of all strains was inhibited by the lowest combination of kanamycin/cefalexin concentrations tested (4 µg/mL of kanamycin and 0.4 µg/mL of cefalexin). One strain assigned to CC20, ST389, and t2094 exhibited resistance to penicillin, oxacillin, and pirlimycin as well as intermediate susceptibility to erythromycin and high minimum inhibitory concentration for several antimicrobial agents, for which no breakpoints were available. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/30799111/Staphylococcus_aureus_related_to_bovine_mastitis_in_Switzerland:_Clonal_diversity_virulence_gene_profiles_and_antimicrobial_resistance_of_isolates_collected_throughout_2017_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(19)30161-4 DB - PRIME DP - Unbound Medicine ER -