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IPIP27 Coordinates PtdIns(4,5)P2 Homeostasis for Successful Cytokinesis.
Curr Biol 2019; 29(5):775-789.e7CB

Abstract

During cytokinesis, an actomyosin contractile ring drives the separation of the two daughter cells. A key molecule in this process is the inositol lipid PtdIns(4,5)P2, which recruits numerous factors to the equatorial region for contractile ring assembly. Despite the importance of PtdIns(4,5)P2 in cytokinesis, the regulation of this lipid in cell division remains poorly understood. Here, we identify a role for IPIP27 in mediating cellular PtdIns(4,5)P2 homeostasis. IPIP27 scaffolds the inositol phosphatase oculocerebrorenal syndrome of Lowe (OCRL) by coupling it to endocytic BAR domain proteins. Loss of IPIP27 causes accumulation of PtdIns(4,5)P2 on aberrant endomembrane vacuoles, mislocalization of the cytokinetic machinery, and extensive cortical membrane blebbing. This phenotype is observed in Drosophila and human cells and can result in cytokinesis failure. We have therefore identified IPIP27 as a key modulator of cellular PtdIns(4,5)P2 homeostasis required for normal cytokinesis. The results indicate that scaffolding of inositol phosphatase activity is critical for maintaining PtdIns(4,5)P2 homeostasis and highlight a critical role for this process in cell division.

Authors+Show Affiliations

School of Biology, Faculty of Biology, Medicine and Health, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK; Department of Pathology and Cell Biology, Université de Montréal, and Sainte-Justine Hospital Research Center, Montréal, QC H3T 1C5, Canada.Cellular Mechanisms of Morphogenesis during Mitosis and Cell Motility Division, Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC H3C 3J7, Canada; Department of Biology, New York University, New York, NY 10003, USA; Center for Genomics and Systems Biology, New York University Abu Dhabi, Saadiyat Island, Abu Dhabi, United Arab Emirates.School of Biology, Faculty of Biology, Medicine and Health, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.Department of Biology, University of York, York YO10 5DD, UK.Department of Pathology and Cell Biology, Université de Montréal, and Sainte-Justine Hospital Research Center, Montréal, QC H3T 1C5, Canada.Cellular Mechanisms of Morphogenesis during Mitosis and Cell Motility Division, Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC H3C 3J7, Canada. Electronic address: sebastien.carreno@umontreal.ca.School of Biology, Faculty of Biology, Medicine and Health, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK. Electronic address: martin.lowe@manchester.ac.uk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30799246

Citation

Carim, Sabrya C., et al. "IPIP27 Coordinates PtdIns(4,5)P2 Homeostasis for Successful Cytokinesis." Current Biology : CB, vol. 29, no. 5, 2019, pp. 775-789.e7.
Carim SC, Ben El Kadhi K, Yan G, et al. IPIP27 Coordinates PtdIns(4,5)P2 Homeostasis for Successful Cytokinesis. Curr Biol. 2019;29(5):775-789.e7.
Carim, S. C., Ben El Kadhi, K., Yan, G., Sweeney, S. T., Hickson, G. R., Carréno, S., & Lowe, M. (2019). IPIP27 Coordinates PtdIns(4,5)P2 Homeostasis for Successful Cytokinesis. Current Biology : CB, 29(5), pp. 775-789.e7. doi:10.1016/j.cub.2019.01.043.
Carim SC, et al. IPIP27 Coordinates PtdIns(4,5)P2 Homeostasis for Successful Cytokinesis. Curr Biol. 2019 03 4;29(5):775-789.e7. PubMed PMID: 30799246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IPIP27 Coordinates PtdIns(4,5)P2 Homeostasis for Successful Cytokinesis. AU - Carim,Sabrya C, AU - Ben El Kadhi,Khaled, AU - Yan,Guanhua, AU - Sweeney,Sean T, AU - Hickson,Gilles R, AU - Carréno,Sébastien, AU - Lowe,Martin, Y1 - 2019/02/21/ PY - 2018/05/18/received PY - 2018/12/03/revised PY - 2019/01/15/accepted PY - 2019/2/26/pubmed PY - 2019/2/26/medline PY - 2019/2/26/entrez KW - BAR domain protein KW - IPIP27 KW - OCRL KW - PtdIns(4,5)P(2) KW - SH3PX1 KW - actin KW - cytokinesis KW - endosome KW - phosphoinositide SP - 775 EP - 789.e7 JF - Current biology : CB JO - Curr. Biol. VL - 29 IS - 5 N2 - During cytokinesis, an actomyosin contractile ring drives the separation of the two daughter cells. A key molecule in this process is the inositol lipid PtdIns(4,5)P2, which recruits numerous factors to the equatorial region for contractile ring assembly. Despite the importance of PtdIns(4,5)P2 in cytokinesis, the regulation of this lipid in cell division remains poorly understood. Here, we identify a role for IPIP27 in mediating cellular PtdIns(4,5)P2 homeostasis. IPIP27 scaffolds the inositol phosphatase oculocerebrorenal syndrome of Lowe (OCRL) by coupling it to endocytic BAR domain proteins. Loss of IPIP27 causes accumulation of PtdIns(4,5)P2 on aberrant endomembrane vacuoles, mislocalization of the cytokinetic machinery, and extensive cortical membrane blebbing. This phenotype is observed in Drosophila and human cells and can result in cytokinesis failure. We have therefore identified IPIP27 as a key modulator of cellular PtdIns(4,5)P2 homeostasis required for normal cytokinesis. The results indicate that scaffolding of inositol phosphatase activity is critical for maintaining PtdIns(4,5)P2 homeostasis and highlight a critical role for this process in cell division. SN - 1879-0445 UR - https://www.unboundmedicine.com/medline/citation/30799246/IPIP27_Coordinates_PtdIns_45_P2_Homeostasis_for_Successful_Cytokinesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-9822(19)30072-7 DB - PRIME DP - Unbound Medicine ER -