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Serum miR-30c-5p is a potential biomarker for multiple system atrophy.
Mol Biol Rep. 2019 Apr; 46(2):1661-1666.MB

Abstract

Multiple system atrophy (MSA) is a neurodegenerative disease that belongs to the α synucleinopathies. Clinically, there is an overlap between MSA and Parkinson's disease (PD), especially at the early disease stage. However, these two pathologies differ in terms of disease progression. Currently, no biomarker exists to differentiate MSA from PD. MicroRNAs are non-coding RNAs implicated in gene expression regulation. MiRNAs modulate cellular activity and they control a range of physiological and pathological functions. miRNAs are found in biofluids, such as blood, serum, plasma, saliva, and cerebrospinal fluid. Many groups, including ours, found that circulating miRNAs are differently expressed in blood, plasma, serum and cerebrospinal fluid of PD and MSA patients. In the present study, our primary aim was to determine if serum mir-30-5p and mir-148b-5p can be used as biomarkers for early diagnosis of PD and/or MSA. Our secondary goal was to determine if serum levels of those miRNAs can be correlated with the patients' clinical profile. Using quantitative PCR (qPCR), we evaluated expression levels of miR-30c-5p and miR148b-5p in serum samples from PD (n = 56), MSA (n = 49), and healthy control (n = 50) subjects. We have found that miR-30c-5p is significantly upregulated in MSA if compared with PD and healthy control subjects. Moreover, serum miR-30c-5p levels correlate with disease duration in both MSA and PD. No significant difference was found in miR-148b-5p among MSA, PD and healthy control subjects. Our results suggest a possible role of serum miR-30-5p as a biomarker for diagnosis and progression of MSA.

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Authors+Show Affiliations

Vallelunga A
Neuroscience Section, Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Salerno, Italy. vallelungaannamaria@gmail.com.
Iannitti T
KWS BioTest, Marine View Office Park, Portishead, Somerset, BS20 7AW, UK.
Dati G
Neuroscience Section, Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Salerno, Italy.
Capece S
Neuroscience Section, Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Salerno, Italy.
Maugeri M
Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Tocci E
Laboratorio Analisi PO Serra San Bruno, ASP Vibo Valentia, Vibo Valentia, Italy.
Picillo M
Neuroscience Section, Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Salerno, Italy.
Volpe G
Clinica Neurologica, AOU San Giovanni di Dio e Ruggi d'Aragona, Salerno, Italy.
Cozzolino A
AO San Giuseppe Moscati, Avellino, Italy.
Squillante M
Clinica Neurologica, AOU San Giovanni di Dio e Ruggi d'Aragona, Salerno, Italy.
Cicarelli G
AO San Giuseppe Moscati, Avellino, Italy.
Barone P
Neuroscience Section, Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Salerno, Italy.
Pellecchia MT
Neuroscience Section, Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Salerno, Italy.

MeSH

AgedBiomarkersDisease ProgressionFemaleGene Expression ProfilingGene Expression RegulationHumansMaleMicroRNAsMiddle AgedMultiple System AtrophyParkinson DiseaseReal-Time Polymerase Chain ReactionTranscriptomeUp-Regulation

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30810945

Citation

Vallelunga, Annamaria, et al. "Serum miR-30c-5p Is a Potential Biomarker for Multiple System Atrophy." Molecular Biology Reports, vol. 46, no. 2, 2019, pp. 1661-1666.
Vallelunga A, Iannitti T, Dati G, et al. Serum miR-30c-5p is a potential biomarker for multiple system atrophy. Mol Biol Rep. 2019;46(2):1661-1666.
Vallelunga, A., Iannitti, T., Dati, G., Capece, S., Maugeri, M., Tocci, E., Picillo, M., Volpe, G., Cozzolino, A., Squillante, M., Cicarelli, G., Barone, P., & Pellecchia, M. T. (2019). Serum miR-30c-5p is a potential biomarker for multiple system atrophy. Molecular Biology Reports, 46(2), 1661-1666. https://doi.org/10.1007/s11033-019-04614-z
Vallelunga A, et al. Serum miR-30c-5p Is a Potential Biomarker for Multiple System Atrophy. Mol Biol Rep. 2019;46(2):1661-1666. PubMed PMID: 30810945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum miR-30c-5p is a potential biomarker for multiple system atrophy. AU - Vallelunga,Annamaria, AU - Iannitti,Tommaso, AU - Dati,Giovanna, AU - Capece,Sabrina, AU - Maugeri,Marco, AU - Tocci,Ersilia, AU - Picillo,Marina, AU - Volpe,Giampiero, AU - Cozzolino,Autilia, AU - Squillante,Massimo, AU - Cicarelli,Giulio, AU - Barone,Paolo, AU - Pellecchia,Maria Teresa, Y1 - 2019/02/27/ PY - 2018/08/28/received PY - 2019/01/18/accepted PY - 2019/2/28/pubmed PY - 2019/9/10/medline PY - 2019/2/28/entrez KW - Biomarker KW - MiR-30c-5p KW - MiRNAs KW - Multiple system atrophy KW - Parkinson’s disease KW - Synucleinopathies SP - 1661 EP - 1666 JF - Molecular biology reports JO - Mol Biol Rep VL - 46 IS - 2 N2 - Multiple system atrophy (MSA) is a neurodegenerative disease that belongs to the α synucleinopathies. Clinically, there is an overlap between MSA and Parkinson's disease (PD), especially at the early disease stage. However, these two pathologies differ in terms of disease progression. Currently, no biomarker exists to differentiate MSA from PD. MicroRNAs are non-coding RNAs implicated in gene expression regulation. MiRNAs modulate cellular activity and they control a range of physiological and pathological functions. miRNAs are found in biofluids, such as blood, serum, plasma, saliva, and cerebrospinal fluid. Many groups, including ours, found that circulating miRNAs are differently expressed in blood, plasma, serum and cerebrospinal fluid of PD and MSA patients. In the present study, our primary aim was to determine if serum mir-30-5p and mir-148b-5p can be used as biomarkers for early diagnosis of PD and/or MSA. Our secondary goal was to determine if serum levels of those miRNAs can be correlated with the patients' clinical profile. Using quantitative PCR (qPCR), we evaluated expression levels of miR-30c-5p and miR148b-5p in serum samples from PD (n = 56), MSA (n = 49), and healthy control (n = 50) subjects. We have found that miR-30c-5p is significantly upregulated in MSA if compared with PD and healthy control subjects. Moreover, serum miR-30c-5p levels correlate with disease duration in both MSA and PD. No significant difference was found in miR-148b-5p among MSA, PD and healthy control subjects. Our results suggest a possible role of serum miR-30-5p as a biomarker for diagnosis and progression of MSA. SN - 1573-4978 UR - https://www.unboundmedicine.com/medline/citation/30810945/Serum_miR_30c_5p_is_a_potential_biomarker_for_multiple_system_atrophy_ DB - PRIME DP - Unbound Medicine ER -