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Antioxidant Activity Evaluation of Dietary Flavonoid Hyperoside Using Saccharomyces Cerevisiae as a Model.
Molecules. 2019 Feb 22; 24(4)M

Abstract

Oxidative stress leads to various diseases, including diabetes, cardiovascular diseases, neurodegenerative diseases, and even cancer. The dietary flavonol glycoside, hyperoside (quercetin-3-O-galactoside), exerts health benefits by preventing oxidative damage. To further understand its antioxidative defence mechanisms, we systemically investigated the regulation of hyperoside on oxidative damage induced by hydrogen peroxide, carbon tetrachloride, and cadmium in Saccharomyces cerevisiae. Hyperoside significantly increased cell viability, decreased lipid peroxidation, and lowered intracellular reactive oxygen species (ROS) levels in the wild-type strain (WT) and mutants gtt1∆ and gtt2∆. However, the strain with ctt1∆ showed variable cell viability and intracellular ROS-scavenging ability in response to the hyperoside treatment upon the stimulation of H₂O₂ and CCl₄. In addition, hyperoside did not confer viability tolerance or intercellular ROS in CdSO₄-induced stress to strains of sod1∆ and gsh1∆. The results suggest that the antioxidative reactions of hyperoside in S. cerevisiae depend on the intercellular ROS detoxification system.

Authors+Show Affiliations

Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. gyt19920908@126.com.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. flianying@126.com.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. wangxiangxing@kj-lab.cn.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. 13820344450@163.com.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. wangmeiyan@tjcu.edu.cn.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. dugang@tjcu.edu.cn.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. guanwenqiang@tjcu.edu.cn.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. ljf@tjcu.edu.cn.Centre for Food Research and Innovation, Department of Wine, Food and Molecular Bioscience, Lincoln University, Lincoln 7647, New Zealand. Margaret.Brennan@lincoln.ac.nz.The Third Central Clinical College, Tianjin Medical University, Jintang Road, Hedong, Tianjin 300170, China. kuohx@hotmail.com.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. Charles.Brennan@lincoln.ac.nz. Centre for Food Research and Innovation, Department of Wine, Food and Molecular Bioscience, Lincoln University, Lincoln 7647, New Zealand. Charles.Brennan@lincoln.ac.nz.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. zhaohui@tjcu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30813233

Citation

Gao, Yuting, et al. "Antioxidant Activity Evaluation of Dietary Flavonoid Hyperoside Using Saccharomyces Cerevisiae as a Model." Molecules (Basel, Switzerland), vol. 24, no. 4, 2019.
Gao Y, Fang L, Wang X, et al. Antioxidant Activity Evaluation of Dietary Flavonoid Hyperoside Using Saccharomyces Cerevisiae as a Model. Molecules. 2019;24(4).
Gao, Y., Fang, L., Wang, X., Lan, R., Wang, M., Du, G., Guan, W., Liu, J., Brennan, M., Guo, H., Brennan, C., & Zhao, H. (2019). Antioxidant Activity Evaluation of Dietary Flavonoid Hyperoside Using Saccharomyces Cerevisiae as a Model. Molecules (Basel, Switzerland), 24(4). https://doi.org/10.3390/molecules24040788
Gao Y, et al. Antioxidant Activity Evaluation of Dietary Flavonoid Hyperoside Using Saccharomyces Cerevisiae as a Model. Molecules. 2019 Feb 22;24(4) PubMed PMID: 30813233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant Activity Evaluation of Dietary Flavonoid Hyperoside Using Saccharomyces Cerevisiae as a Model. AU - Gao,Yuting, AU - Fang,Lianying, AU - Wang,Xiangxing, AU - Lan,Ruoni, AU - Wang,Meiyan, AU - Du,Gang, AU - Guan,Wenqiang, AU - Liu,Jianfu, AU - Brennan,Margaret, AU - Guo,Hongxing, AU - Brennan,Charles, AU - Zhao,Hui, Y1 - 2019/02/22/ PY - 2019/01/24/received PY - 2019/02/19/revised PY - 2019/02/21/accepted PY - 2019/3/1/entrez PY - 2019/3/1/pubmed PY - 2019/6/14/medline KW - Saccharomyces cerevisiae KW - hyperoside KW - intracellular ROS KW - lipid peroxidation KW - oxidative damage JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 4 N2 - Oxidative stress leads to various diseases, including diabetes, cardiovascular diseases, neurodegenerative diseases, and even cancer. The dietary flavonol glycoside, hyperoside (quercetin-3-O-galactoside), exerts health benefits by preventing oxidative damage. To further understand its antioxidative defence mechanisms, we systemically investigated the regulation of hyperoside on oxidative damage induced by hydrogen peroxide, carbon tetrachloride, and cadmium in Saccharomyces cerevisiae. Hyperoside significantly increased cell viability, decreased lipid peroxidation, and lowered intracellular reactive oxygen species (ROS) levels in the wild-type strain (WT) and mutants gtt1∆ and gtt2∆. However, the strain with ctt1∆ showed variable cell viability and intracellular ROS-scavenging ability in response to the hyperoside treatment upon the stimulation of H₂O₂ and CCl₄. In addition, hyperoside did not confer viability tolerance or intercellular ROS in CdSO₄-induced stress to strains of sod1∆ and gsh1∆. The results suggest that the antioxidative reactions of hyperoside in S. cerevisiae depend on the intercellular ROS detoxification system. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30813233/Antioxidant_Activity_Evaluation_of_Dietary_Flavonoid_Hyperoside_Using_Saccharomyces_Cerevisiae_as_a_Model_ L2 - https://www.mdpi.com/resolver?pii=molecules24040788 DB - PRIME DP - Unbound Medicine ER -