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Comparative Proteome-Wide Analysis of Bone Marrow Microenvironment of β-Thalassemia/Hemoglobin E.
Proteomes 2019; 7(1)P

Abstract

β-thalassemia/Hb E is a global health issue, which is characterized by a range of clinical symptoms from a mild and asymptomatic anemia to severe disorders that require transfusions from infancy. Pathological mechanisms of the disease involve the excess of unmatched alpha globin and iron overload, leading to ineffective erythropoiesis and ultimately to the premature death of erythroid precursors in bone marrow (BM) and peripheral organs. However, it is unclear as to how BM microenvironment factors contribute to the defective erythropoiesis in β-thalassemia/Hb E patients. Here, we employed mass spectrometry-based comparative proteomics to analyze BM plasma that was collected from six β-thalassemia/Hb E patients and four healthy donors. We identified that the differentially expressed proteins are enriched in secretory or exosome-associated proteins, many of which have putative functions in the oxidative stress response. Using Western blot assay, we confirmed that atypical lipoprotein, Apolipoprotein D (APOD), belonging to the Lipocalin transporter superfamily, was significantly decreased in BM plasma of the tested pediatric β-thalassemia/Hb E patients. Our results highlight that the disease condition of ineffective erythropoiesis and oxidative stress found in BM microenvironment of β-thalassemia/Hb E patients is associated with the impaired expression of APOD protein.

Authors+Show Affiliations

Chulabhorn International College of Medicine, Thammasat University Rangsit campus, Pathum Thani 12121, Thailand. saranyoo@tu.ac.th.Chulabhorn International College of Medicine, Thammasat University Rangsit campus, Pathum Thani 12121, Thailand. scrmr303@gmail.com.Faculty of Medical Technology, Rangsit University, Pathum Thani 12000, Thailand. suwit_klong51@hotmail.com.National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Pathum Thani 12121, Thailand. sittiruk@biotec.or.th.Chulabhorn International College of Medicine, Thammasat University Rangsit campus, Pathum Thani 12121, Thailand. kitima_su@hotmail.com. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. kitima_su@hotmail.com.Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. pexcotung@gmail.com.Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. suradej.hon@mahidol.ac.th.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30813444

Citation

Ponnikorn, Saranyoo, et al. "Comparative Proteome-Wide Analysis of Bone Marrow Microenvironment of β-Thalassemia/Hemoglobin E." Proteomes, vol. 7, no. 1, 2019.
Ponnikorn S, Mongkolrob R, Klongthalay S, et al. Comparative Proteome-Wide Analysis of Bone Marrow Microenvironment of β-Thalassemia/Hemoglobin E. Proteomes. 2019;7(1).
Ponnikorn, S., Mongkolrob, R., Klongthalay, S., Roytrakul, S., Srisanga, K., Tungpradabkul, S., & Hongeng, S. (2019). Comparative Proteome-Wide Analysis of Bone Marrow Microenvironment of β-Thalassemia/Hemoglobin E. Proteomes, 7(1), doi:10.3390/proteomes7010008.
Ponnikorn S, et al. Comparative Proteome-Wide Analysis of Bone Marrow Microenvironment of β-Thalassemia/Hemoglobin E. Proteomes. 2019 Feb 23;7(1) PubMed PMID: 30813444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Proteome-Wide Analysis of Bone Marrow Microenvironment of β-Thalassemia/Hemoglobin E. AU - Ponnikorn,Saranyoo, AU - Mongkolrob,Rungrawee, AU - Klongthalay,Suwit, AU - Roytrakul,Sittiruk, AU - Srisanga,Kitima, AU - Tungpradabkul,Sumalee, AU - Hongeng,Suradej, Y1 - 2019/02/23/ PY - 2018/12/13/received PY - 2019/02/16/revised PY - 2019/02/19/accepted PY - 2019/3/1/entrez PY - 2019/3/1/pubmed PY - 2019/3/1/medline KW - Apolipoprotein D KW - bone marrow KW - ineffective erythropoiesis KW - oxidative stress KW - proteomics KW - β-thalassemia/Hb E JF - Proteomes JO - Proteomes VL - 7 IS - 1 N2 - β-thalassemia/Hb E is a global health issue, which is characterized by a range of clinical symptoms from a mild and asymptomatic anemia to severe disorders that require transfusions from infancy. Pathological mechanisms of the disease involve the excess of unmatched alpha globin and iron overload, leading to ineffective erythropoiesis and ultimately to the premature death of erythroid precursors in bone marrow (BM) and peripheral organs. However, it is unclear as to how BM microenvironment factors contribute to the defective erythropoiesis in β-thalassemia/Hb E patients. Here, we employed mass spectrometry-based comparative proteomics to analyze BM plasma that was collected from six β-thalassemia/Hb E patients and four healthy donors. We identified that the differentially expressed proteins are enriched in secretory or exosome-associated proteins, many of which have putative functions in the oxidative stress response. Using Western blot assay, we confirmed that atypical lipoprotein, Apolipoprotein D (APOD), belonging to the Lipocalin transporter superfamily, was significantly decreased in BM plasma of the tested pediatric β-thalassemia/Hb E patients. Our results highlight that the disease condition of ineffective erythropoiesis and oxidative stress found in BM microenvironment of β-thalassemia/Hb E patients is associated with the impaired expression of APOD protein. SN - 2227-7382 UR - https://www.unboundmedicine.com/medline/citation/30813444/Comparative_Proteome-Wide_Analysis_of_Bone_Marrow_Microenvironment_of_β-Thalassemia/Hemoglobin_E L2 - http://www.mdpi.com/resolver?pii=proteomes7010008 DB - PRIME DP - Unbound Medicine ER -