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Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes.
Turk J Biol. 2018; 42(2):152-162.TJ

Abstract

Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was to investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts of Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid (gallic, vanillic, caefic, chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treated with extracts of both plants separately and in combinations with mitomycin C (MMC). In separate treatments, both herbal extracts significantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of T. scordium , except the lowest, and all concentrations of T. polium extracts in combined treatment with MMC significantly reduced the frequency of MN. The extract of T. polium did not significantly aefct the nuclear division index (NDI), whereas T. scordium in higher concentrations, separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts in combination with MMC have antimutagenic (T. polium) and proapoptotic effects (T. scordium), which indicates their protective effects in PBLs.

Authors+Show Affiliations

Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia. Department of Genetics, Faculty of Medical Sciences, University of Kragujevac , Kragujevac , Serbia.Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.Department of Chemistry, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.Serbian Academy of Sciences and Arts , Belgrade , Serbia.Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30814877

Citation

Milošević-Djordjević, Olivera, et al. "Polyphenolic Contents of Teucrium Polium L. and Teucrium Scordium L. Associated With Their Protective Effects Against MMC-induced Chromosomal Damage in Cultured Human Peripheral Blood Lymphocytes." Turkish Journal of Biology = Turk Biyoloji Dergisi, vol. 42, no. 2, 2018, pp. 152-162.
Milošević-Djordjević O, Radović Jakovljević M, Marković A, et al. Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes. Turk J Biol. 2018;42(2):152-162.
Milošević-Djordjević, O., Radović Jakovljević, M., Marković, A., Stanković, M., Ćirić, A., Marinković, D., & Grujičić, D. (2018). Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes. Turkish Journal of Biology = Turk Biyoloji Dergisi, 42(2), 152-162. https://doi.org/10.3906/biy-1707-36
Milošević-Djordjević O, et al. Polyphenolic Contents of Teucrium Polium L. and Teucrium Scordium L. Associated With Their Protective Effects Against MMC-induced Chromosomal Damage in Cultured Human Peripheral Blood Lymphocytes. Turk J Biol. 2018;42(2):152-162. PubMed PMID: 30814877.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes. AU - Milošević-Djordjević,Olivera, AU - Radović Jakovljević,Marina, AU - Marković,Aleksandra, AU - Stanković,Milan, AU - Ćirić,Andrija, AU - Marinković,Dragoslav, AU - Grujičić,Darko, Y1 - 2018/04/27/ PY - 2019/3/1/entrez PY - 2019/3/1/pubmed PY - 2019/3/1/medline KW - Genotoxic potential KW - HPLC analysis KW - Teucrium polium KW - Teucrium scordium KW - human peripheral blood lymphocytes KW - micronuclei SP - 152 EP - 162 JF - Turkish journal of biology = Turk biyoloji dergisi JO - Turk J Biol VL - 42 IS - 2 N2 - Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was to investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts of Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid (gallic, vanillic, caefic, chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treated with extracts of both plants separately and in combinations with mitomycin C (MMC). In separate treatments, both herbal extracts significantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of T. scordium , except the lowest, and all concentrations of T. polium extracts in combined treatment with MMC significantly reduced the frequency of MN. The extract of T. polium did not significantly aefct the nuclear division index (NDI), whereas T. scordium in higher concentrations, separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts in combination with MMC have antimutagenic (T. polium) and proapoptotic effects (T. scordium), which indicates their protective effects in PBLs. SN - 1303-6092 UR - https://www.unboundmedicine.com/medline/citation/30814877/Polyphenolic_contents_of_Teucrium_polium_L__and_Teucrium_scordium_L__associated_with_their_protective_effects_against_MMC_induced_chromosomal_damage_in_cultured_human_peripheral_blood_lymphocytes_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30814877/ DB - PRIME DP - Unbound Medicine ER -