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Elucidating fentanyls differentiation from morphines in chemical and biological samples with surface-enhanced Raman spectroscopy.
Electrophoresis. 2019 08; 40(16-17):2193-2203.E

Abstract

Fentanyl and morphine are opioid drugs as well as new psychoactive substances. Even originally introduced as efficient anesthetic drugs to relieve moderate-to-severe pain in clinic, the overdose of new synthetic opioids is currently a serious public health problem in numerous countries worldwide. The entire category of fentanyls has been included in the regulatory list in several countries. There is a great and urgent demand to rapidly recognize fentanyls and morphines in various samples. Here, we report an on-site surface-enhanced Raman spectroscopic method to classify fentanyls from morphines by the Raman spectroscopic signature of the molecular scaffold structure, with an assistance of principle component analysis algorithm. Moreover, by simple but fine-tuning approach of inorganic salt-induced aggregation of gold nanoparticles substrate, we achieved a selective detection of 10 ng/mL fentanyl from 2000-fold of heroin, the most common coexisting substance in chemical samples. Good differentiation of 50 ng/mL fentanyl from 10 000-fold morphine as a main metabolite of heroin in urine samples was also possible after a feasible pretreatment by StageTip procedures. Depending on different structures, the detection sensitivity of five fentanyls ranged from 50 to 2000 ng/mL.

Authors+Show Affiliations

State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Services, Beijing, P. R. China.State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Services, Beijing, P. R. China.State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Services, Beijing, P. R. China.State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Services, Beijing, P. R. China.State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Services, Beijing, P. R. China.State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Services, Beijing, P. R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30815884

Citation

Wang, Kai, et al. "Elucidating Fentanyls Differentiation From Morphines in Chemical and Biological Samples With Surface-enhanced Raman Spectroscopy." Electrophoresis, vol. 40, no. 16-17, 2019, pp. 2193-2203.
Wang K, Xu B, Wu J, et al. Elucidating fentanyls differentiation from morphines in chemical and biological samples with surface-enhanced Raman spectroscopy. Electrophoresis. 2019;40(16-17):2193-2203.
Wang, K., Xu, B., Wu, J., Zhu, Y., Guo, L., & Xie, J. (2019). Elucidating fentanyls differentiation from morphines in chemical and biological samples with surface-enhanced Raman spectroscopy. Electrophoresis, 40(16-17), 2193-2203. https://doi.org/10.1002/elps.201900004
Wang K, et al. Elucidating Fentanyls Differentiation From Morphines in Chemical and Biological Samples With Surface-enhanced Raman Spectroscopy. Electrophoresis. 2019;40(16-17):2193-2203. PubMed PMID: 30815884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elucidating fentanyls differentiation from morphines in chemical and biological samples with surface-enhanced Raman spectroscopy. AU - Wang,Kai, AU - Xu,Bin, AU - Wu,Jianfeng, AU - Zhu,Yingjie, AU - Guo,Lei, AU - Xie,Jianwei, Y1 - 2019/03/13/ PY - 2019/01/02/received PY - 2019/02/13/revised PY - 2019/02/14/accepted PY - 2019/3/1/pubmed PY - 2020/3/5/medline PY - 2019/3/1/entrez KW - Differentiation KW - Fentanyls KW - Morphines KW - SERS KW - Urine SP - 2193 EP - 2203 JF - Electrophoresis JO - Electrophoresis VL - 40 IS - 16-17 N2 - Fentanyl and morphine are opioid drugs as well as new psychoactive substances. Even originally introduced as efficient anesthetic drugs to relieve moderate-to-severe pain in clinic, the overdose of new synthetic opioids is currently a serious public health problem in numerous countries worldwide. The entire category of fentanyls has been included in the regulatory list in several countries. There is a great and urgent demand to rapidly recognize fentanyls and morphines in various samples. Here, we report an on-site surface-enhanced Raman spectroscopic method to classify fentanyls from morphines by the Raman spectroscopic signature of the molecular scaffold structure, with an assistance of principle component analysis algorithm. Moreover, by simple but fine-tuning approach of inorganic salt-induced aggregation of gold nanoparticles substrate, we achieved a selective detection of 10 ng/mL fentanyl from 2000-fold of heroin, the most common coexisting substance in chemical samples. Good differentiation of 50 ng/mL fentanyl from 10 000-fold morphine as a main metabolite of heroin in urine samples was also possible after a feasible pretreatment by StageTip procedures. Depending on different structures, the detection sensitivity of five fentanyls ranged from 50 to 2000 ng/mL. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/30815884/Elucidating_fentanyls_differentiation_from_morphines_in_chemical_and_biological_samples_with_surface_enhanced_Raman_spectroscopy_ L2 - https://doi.org/10.1002/elps.201900004 DB - PRIME DP - Unbound Medicine ER -