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Comparative distribution of 3H-GABA uptake and GAD immunoreactivity in goldfish retinal amacrine cells: a double-label analysis.
J Comp Neurol 1986; 244(2):149-62JC

Abstract

The comparative distribution of 3H-GABA uptake and glutamic acid decarboxylase immunoreactivity (GAD-IR) in amacrine cells of goldfish retina was studied simultaneously by using a combined autoradiographic/immunocytochemical technique in order to determine the degree of colocalization of these two markers of GABAergic neurons; 3H-GABA was taken up most intensely by large pyriform Ab amacrine cells (3% of inner nuclear layer (INL) somata), and less intensely by smaller, polyform amacrine cells (12% of INL somata), cell bodies in the ganglion cell layer (one-half as common as Ab cells), and cell bodies in the inner plexiform layer (very rare). GAD-IR was observed in 25% of amacrine cells in the INL, the vast majority of which were polyform in shape, and cell bodies in the ganglion cell layer. Twice as many cells were labeled for GAD-IR as for 3H-GABA uptake. Of the cells that took up 3H-GABA, colocalization of 3H-GABA uptake and GAD-IR was observed in 90% of the polyform amacrine cells, 80% of the cells in the ganglion cell layer, and none of the pyriform Ab amacrine cells or cells in the inner plexiform layer. We suggest that the polyform cells compose the major population of GABAergic amacrine cells in the goldfish retina, rather than the pyriform Ab cells. GABAergic displaced amacrine cells or ganglion cells are also indicated by our data. The implications of these data with regard to the physiology of the goldfish retina are discussed as well.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3081603

Citation

Yazulla, S, et al. "Comparative Distribution of 3H-GABA Uptake and GAD Immunoreactivity in Goldfish Retinal Amacrine Cells: a Double-label Analysis." The Journal of Comparative Neurology, vol. 244, no. 2, 1986, pp. 149-62.
Yazulla S, Studholme K, Wu JY. Comparative distribution of 3H-GABA uptake and GAD immunoreactivity in goldfish retinal amacrine cells: a double-label analysis. J Comp Neurol. 1986;244(2):149-62.
Yazulla, S., Studholme, K., & Wu, J. Y. (1986). Comparative distribution of 3H-GABA uptake and GAD immunoreactivity in goldfish retinal amacrine cells: a double-label analysis. The Journal of Comparative Neurology, 244(2), pp. 149-62.
Yazulla S, Studholme K, Wu JY. Comparative Distribution of 3H-GABA Uptake and GAD Immunoreactivity in Goldfish Retinal Amacrine Cells: a Double-label Analysis. J Comp Neurol. 1986 Feb 8;244(2):149-62. PubMed PMID: 3081603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative distribution of 3H-GABA uptake and GAD immunoreactivity in goldfish retinal amacrine cells: a double-label analysis. AU - Yazulla,S, AU - Studholme,K, AU - Wu,J Y, PY - 1986/2/8/pubmed PY - 1986/2/8/medline PY - 1986/2/8/entrez SP - 149 EP - 62 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 244 IS - 2 N2 - The comparative distribution of 3H-GABA uptake and glutamic acid decarboxylase immunoreactivity (GAD-IR) in amacrine cells of goldfish retina was studied simultaneously by using a combined autoradiographic/immunocytochemical technique in order to determine the degree of colocalization of these two markers of GABAergic neurons; 3H-GABA was taken up most intensely by large pyriform Ab amacrine cells (3% of inner nuclear layer (INL) somata), and less intensely by smaller, polyform amacrine cells (12% of INL somata), cell bodies in the ganglion cell layer (one-half as common as Ab cells), and cell bodies in the inner plexiform layer (very rare). GAD-IR was observed in 25% of amacrine cells in the INL, the vast majority of which were polyform in shape, and cell bodies in the ganglion cell layer. Twice as many cells were labeled for GAD-IR as for 3H-GABA uptake. Of the cells that took up 3H-GABA, colocalization of 3H-GABA uptake and GAD-IR was observed in 90% of the polyform amacrine cells, 80% of the cells in the ganglion cell layer, and none of the pyriform Ab amacrine cells or cells in the inner plexiform layer. We suggest that the polyform cells compose the major population of GABAergic amacrine cells in the goldfish retina, rather than the pyriform Ab cells. GABAergic displaced amacrine cells or ganglion cells are also indicated by our data. The implications of these data with regard to the physiology of the goldfish retina are discussed as well. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/3081603/Comparative_distribution_of_3H_GABA_uptake_and_GAD_immunoreactivity_in_goldfish_retinal_amacrine_cells:_a_double_label_analysis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1986&volume=244&issue=2&spage=149 DB - PRIME DP - Unbound Medicine ER -