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Keratinocyte electrotaxis induced by physiological pulsed direct current electric fields.
Bioelectrochemistry 2019; 127:113-124B

Abstract

Endogenous electric fields (EFs) direct the migration (electrotaxis) of keratinocytes in skin wounds, and the exogenous application of EFs may therefore improve wound healing, but the potential benefits are limited by the side effects of constant direct current (DC) passing through tissues. In contrast, with pulsed DC (characterized by intermittent output), parameters can be adjusted to minimize the adverse effects of electric currents. However, it remains unknown whether pulsed DC can reliably induce keratinocyte electrotaxis. In this study, using primary keratinocytes in an electrotaxis chamber, we found that a pulsed DCEF at physiological strength (EF = 150 mV/mm, duty cycle = 60%, frequency = 0.1 Hz) could induce robust electrotaxis. This effect was dependent on both voltage and duty cycle, but not on frequency. As predicted, fewer electrochemical reactions and cytotoxic reactions were detected with pulsed DCEF than with constant DCEF. In summary, we here demonstrate for the first time, that pulsed DCEF can trigger keratinocyte electrotaxis comparable to that induced by constant DCEF, while minimizing the electrochemical side effects. These findings support the future development of a pulsed DCEF device to improve wound healing in human patients.

Authors+Show Affiliations

Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China.Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing, China.Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing, China.Department of Burn and Plastic Surgery, Xian Center Hospital, Xian, China.Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China.Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing, China.Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China.Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing, China.Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China. Electronic address: fdltmmu@sina.com.Department of Plastic and Cosmetic Surgery, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing, China. Electronic address: japzhang@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30818261

Citation

Ren, Xi, et al. "Keratinocyte Electrotaxis Induced By Physiological Pulsed Direct Current Electric Fields." Bioelectrochemistry (Amsterdam, Netherlands), vol. 127, 2019, pp. 113-124.
Ren X, Sun H, Liu J, et al. Keratinocyte electrotaxis induced by physiological pulsed direct current electric fields. Bioelectrochemistry. 2019;127:113-124.
Ren, X., Sun, H., Liu, J., Guo, X., Huang, J., Jiang, X., ... Zhang, J. (2019). Keratinocyte electrotaxis induced by physiological pulsed direct current electric fields. Bioelectrochemistry (Amsterdam, Netherlands), 127, pp. 113-124. doi:10.1016/j.bioelechem.2019.02.001.
Ren X, et al. Keratinocyte Electrotaxis Induced By Physiological Pulsed Direct Current Electric Fields. Bioelectrochemistry. 2019;127:113-124. PubMed PMID: 30818261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Keratinocyte electrotaxis induced by physiological pulsed direct current electric fields. AU - Ren,Xi, AU - Sun,Huanbo, AU - Liu,Jie, AU - Guo,Xiaowei, AU - Huang,Jingzhuo, AU - Jiang,Xupin, AU - Zhang,Yiming, AU - Huang,Yuesheng, AU - Fan,Dongli, AU - Zhang,Jiaping, Y1 - 2019/02/14/ PY - 2018/11/25/received PY - 2019/02/01/revised PY - 2019/02/01/accepted PY - 2019/3/1/pubmed PY - 2019/4/18/medline PY - 2019/3/1/entrez KW - Constant electric field KW - ERK KW - Electrotaxis KW - Keratinocyte KW - Pulsed electric field KW - Wound healing SP - 113 EP - 124 JF - Bioelectrochemistry (Amsterdam, Netherlands) JO - Bioelectrochemistry VL - 127 N2 - Endogenous electric fields (EFs) direct the migration (electrotaxis) of keratinocytes in skin wounds, and the exogenous application of EFs may therefore improve wound healing, but the potential benefits are limited by the side effects of constant direct current (DC) passing through tissues. In contrast, with pulsed DC (characterized by intermittent output), parameters can be adjusted to minimize the adverse effects of electric currents. However, it remains unknown whether pulsed DC can reliably induce keratinocyte electrotaxis. In this study, using primary keratinocytes in an electrotaxis chamber, we found that a pulsed DCEF at physiological strength (EF = 150 mV/mm, duty cycle = 60%, frequency = 0.1 Hz) could induce robust electrotaxis. This effect was dependent on both voltage and duty cycle, but not on frequency. As predicted, fewer electrochemical reactions and cytotoxic reactions were detected with pulsed DCEF than with constant DCEF. In summary, we here demonstrate for the first time, that pulsed DCEF can trigger keratinocyte electrotaxis comparable to that induced by constant DCEF, while minimizing the electrochemical side effects. These findings support the future development of a pulsed DCEF device to improve wound healing in human patients. SN - 1878-562X UR - https://www.unboundmedicine.com/medline/citation/30818261/Keratinocyte_electrotaxis_induced_by_physiological_pulsed_direct_current_electric_fields L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5394(18)30553-X DB - PRIME DP - Unbound Medicine ER -