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N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei.
Biotechnol Biofuels. 2019; 12:36.BB

Abstract

Background

The filamentous fungus Trichoderma reesei produces cellulase enzymes that are widely studied for lignocellulose bioconversion to biofuel. N,N-dimethylformamide (DMF) is a versatile organic solvent used in large quantities in industries.

Results

In this study, we serendipitously found that biologically relevant concentrations of extracellular DMF-induced cellulase production in the T. reesei hyper-cellulolytic mutant Rut-C30 and wild-type strain QM6a. Next, by transcriptome analysis, we determined that plc-e encoding phospholipase C was activated by DMF and revealed that cytosolic Ca2+ plays a vital role in the response of T. reesei to DMF. Using EGTA (a putative extracellular Ca2+ chelator) and LaCl3 (a plasma membrane Ca2+ channel blocker), we demonstrated that DMF induced a cytosolic Ca2+ burst via extracellular Ca2+ and Ca2+ channels in T. reesei, and that the cytosolic Ca2+ burst induced by DMF-mediated overexpression of cellulase through calcium signaling. Deletion of crz1 confirmed that calcium signaling plays a dominant role in DMF-induced cellulase production. Additionally, 0.5-2% DMF increases the permeability of T. reesei mycelia for cellulase release. Simultaneous supplementation with 1% DMF and 10 mM Mn2+ to T. reesei Rut-C30 increased cellulase activity approximately fourfold compared to that without treatment and was also more than that observed in response to either treatment alone.

Conclusions

Our results reveal that DMF-induced cellulase production via calcium signaling and permeabilization. Our results also provide insight into the role of calcium signaling in enzyme production for enhanced cellulase production and the development of novel inducers of cellulase.

Authors+Show Affiliations

State Key Lab of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237 China.State Key Lab of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237 China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30820246

Citation

Chen, Yumeng, et al. "N,N-dimethylformamide Induces Cellulase Production in the Filamentous Fungus Trichoderma Reesei." Biotechnology for Biofuels, vol. 12, 2019, p. 36.
Chen Y, Wu C, Shen Y, et al. N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei. Biotechnol Biofuels. 2019;12:36.
Chen, Y., Wu, C., Shen, Y., Ma, Y., Wei, D., & Wang, W. (2019). N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei. Biotechnology for Biofuels, 12, 36. https://doi.org/10.1186/s13068-019-1375-1
Chen Y, et al. N,N-dimethylformamide Induces Cellulase Production in the Filamentous Fungus Trichoderma Reesei. Biotechnol Biofuels. 2019;12:36. PubMed PMID: 30820246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei. AU - Chen,Yumeng, AU - Wu,Chuan, AU - Shen,Yaling, AU - Ma,Yushu, AU - Wei,Dongzhi, AU - Wang,Wei, Y1 - 2019/02/19/ PY - 2018/11/02/received PY - 2019/02/08/accepted PY - 2019/3/2/entrez PY - 2019/3/2/pubmed PY - 2019/3/2/medline KW - Calcium signaling KW - Cellulase KW - DMF induced KW - Permeability KW - Phospholipase C KW - Trichoderma reesei KW - plc-e SP - 36 EP - 36 JF - Biotechnology for biofuels JO - Biotechnol Biofuels VL - 12 N2 - Background: The filamentous fungus Trichoderma reesei produces cellulase enzymes that are widely studied for lignocellulose bioconversion to biofuel. N,N-dimethylformamide (DMF) is a versatile organic solvent used in large quantities in industries. Results: In this study, we serendipitously found that biologically relevant concentrations of extracellular DMF-induced cellulase production in the T. reesei hyper-cellulolytic mutant Rut-C30 and wild-type strain QM6a. Next, by transcriptome analysis, we determined that plc-e encoding phospholipase C was activated by DMF and revealed that cytosolic Ca2+ plays a vital role in the response of T. reesei to DMF. Using EGTA (a putative extracellular Ca2+ chelator) and LaCl3 (a plasma membrane Ca2+ channel blocker), we demonstrated that DMF induced a cytosolic Ca2+ burst via extracellular Ca2+ and Ca2+ channels in T. reesei, and that the cytosolic Ca2+ burst induced by DMF-mediated overexpression of cellulase through calcium signaling. Deletion of crz1 confirmed that calcium signaling plays a dominant role in DMF-induced cellulase production. Additionally, 0.5-2% DMF increases the permeability of T. reesei mycelia for cellulase release. Simultaneous supplementation with 1% DMF and 10 mM Mn2+ to T. reesei Rut-C30 increased cellulase activity approximately fourfold compared to that without treatment and was also more than that observed in response to either treatment alone. Conclusions: Our results reveal that DMF-induced cellulase production via calcium signaling and permeabilization. Our results also provide insight into the role of calcium signaling in enzyme production for enhanced cellulase production and the development of novel inducers of cellulase. SN - 1754-6834 UR - https://www.unboundmedicine.com/medline/citation/30820246/NN_dimethylformamide_induces_cellulase_production_in_the_filamentous_fungus_Trichoderma_reesei_ L2 - https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-019-1375-1 DB - PRIME DP - Unbound Medicine ER -
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