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Nuclear sperm quality in total polymorphic teratozoospermia and its impact on intracytoplasmic sperm injection outcome.
Andrologia. 2019 Jun; 51(5):e13252.A

Abstract

Various nuclear sperm alterations are reported in patients with syndromic teratozoospermia; however, this has not been clearly identified yet in total polymorphic teratozoospermia. The aim of this study was to analyse sperm aneuploidy, DNA integrity and chromatin packaging in 45 infertile patients with total polymorphic teratozoospermia, and to compare obtained results with those collected from 25 fertile men. For 14 patients, the impact of nuclear sperm abnormalities on intracytoplasmic sperm injection (ICSI) outcomes was analysed. Sperm chromatin condensation was evaluated using aniline blue staining, DNA fragmentation by TUNEL assay and chromosome abnormalities by FISH. The mean DNA fragmentation index was significantly higher in patients compared to controls, weakly and positively correlated to acrosome defects (r = 0.3; p = 0.04) and positively and moderately correlated to microcephalic heads (r = 0.5; p = 0.027). The aniline blue-reacted spermatozoa rate was also high in comparison with controls, moderately and negatively correlated to progressive motility (r = -0.6; p = 0.014). Total aneuploidy rate was considerably higher in our patients. A positive and moderate correlation was found between disomy Y rate and acrosome abnormalities (r = 0.5; p = 0.048). These patients had an impaired sperm nuclear quality, which will affect the results in ICSI. Therefore, analysis of sperm chromatin condensation, DNA integrity and aneuploidy in such cases is very useful before ART.

Authors+Show Affiliations

Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.Reproductive Medicine Unit, Farhat Hached University Hospital, Sousse, Tunisia. Faculty of Medicine of Sousse, Farhat Hached Hospital, University of Sousse, Sousse, Tunisia.Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia. Faculty of Medicine of Sousse, Farhat Hached Hospital, University of Sousse, Sousse, Tunisia.Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia. Faculty of Medicine of Sousse, Farhat Hached Hospital, University of Sousse, Sousse, Tunisia.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

30821000

Citation

Braham, Asma, et al. "Nuclear Sperm Quality in Total Polymorphic Teratozoospermia and Its Impact On Intracytoplasmic Sperm Injection Outcome." Andrologia, vol. 51, no. 5, 2019, pp. e13252.
Braham A, Ghedir H, Zidi I, et al. Nuclear sperm quality in total polymorphic teratozoospermia and its impact on intracytoplasmic sperm injection outcome. Andrologia. 2019;51(5):e13252.
Braham, A., Ghedir, H., Zidi, I., Sallem, A., Hajlaoui, A., Ajina, M., Saad, A., & Ibala-Romdhane, S. (2019). Nuclear sperm quality in total polymorphic teratozoospermia and its impact on intracytoplasmic sperm injection outcome. Andrologia, 51(5), e13252. https://doi.org/10.1111/and.13252
Braham A, et al. Nuclear Sperm Quality in Total Polymorphic Teratozoospermia and Its Impact On Intracytoplasmic Sperm Injection Outcome. Andrologia. 2019;51(5):e13252. PubMed PMID: 30821000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nuclear sperm quality in total polymorphic teratozoospermia and its impact on intracytoplasmic sperm injection outcome. AU - Braham,Asma, AU - Ghedir,Houda, AU - Zidi,Ines, AU - Sallem,Amira, AU - Hajlaoui,Amani, AU - Ajina,Mounir, AU - Saad,Ali, AU - Ibala-Romdhane,Samira, Y1 - 2019/02/28/ PY - 2018/09/25/received PY - 2019/01/14/revised PY - 2019/01/17/accepted PY - 2019/3/2/pubmed PY - 2019/9/12/medline PY - 2019/3/2/entrez KW - DNA integrity KW - aneuploidy KW - assisted reproductive techniques KW - chromatin condensation KW - teratozoospermia SP - e13252 EP - e13252 JF - Andrologia JO - Andrologia VL - 51 IS - 5 N2 - Various nuclear sperm alterations are reported in patients with syndromic teratozoospermia; however, this has not been clearly identified yet in total polymorphic teratozoospermia. The aim of this study was to analyse sperm aneuploidy, DNA integrity and chromatin packaging in 45 infertile patients with total polymorphic teratozoospermia, and to compare obtained results with those collected from 25 fertile men. For 14 patients, the impact of nuclear sperm abnormalities on intracytoplasmic sperm injection (ICSI) outcomes was analysed. Sperm chromatin condensation was evaluated using aniline blue staining, DNA fragmentation by TUNEL assay and chromosome abnormalities by FISH. The mean DNA fragmentation index was significantly higher in patients compared to controls, weakly and positively correlated to acrosome defects (r = 0.3; p = 0.04) and positively and moderately correlated to microcephalic heads (r = 0.5; p = 0.027). The aniline blue-reacted spermatozoa rate was also high in comparison with controls, moderately and negatively correlated to progressive motility (r = -0.6; p = 0.014). Total aneuploidy rate was considerably higher in our patients. A positive and moderate correlation was found between disomy Y rate and acrosome abnormalities (r = 0.5; p = 0.048). These patients had an impaired sperm nuclear quality, which will affect the results in ICSI. Therefore, analysis of sperm chromatin condensation, DNA integrity and aneuploidy in such cases is very useful before ART. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/30821000/Nuclear_sperm_quality_in_total_polymorphic_teratozoospermia_and_its_impact_on_intracytoplasmic_sperm_injection_outcome_ L2 - https://doi.org/10.1111/and.13252 DB - PRIME DP - Unbound Medicine ER -