Tags

Type your tag names separated by a space and hit enter

Toxicogenomic responses of Caenorhabditis elegans to pristine and transformed zinc oxide nanoparticles.
Environ Pollut. 2019 Apr; 247:917-926.EP

Abstract

Manufactured nanoparticles (MNPs) undergo transformation immediately after they enter wastewater treatment streams and during their partitioning to sewage sludge, which is applied to agricultural soils in form of biosolids. We examined toxicogenomic responses of the model nematode Caenorhabditis elegans to pristine and transformed ZnO-MNPs (phosphatized pZnO- and sulfidized sZnO-MNPs). To account for the toxicity due to dissolved Zn, a ZnSO4 treatment was included. Transformation of ZnO-MNPs reduced their toxicity by nearly ten-fold, while there was almost no difference in the toxicity of pristine ZnO-MNPs and ZnSO4. This combined with the fact that far more dissolved Zn was released from ZnO- compared to pZnO- or sZnO-MNPs, suggests that dissolution of pristine ZnO-MNPs is one of the main drivers of their toxicity. Transcriptomic responses at the EC30 for reproduction resulted in a total of 1161 differentially expressed genes. Fifty percent of the genes differentially expressed in the ZnSO4 treatment, including the three metal responsive genes (mtl-1, mtl-2 and numr-1), were shared among all treatments, suggesting that responses to all forms of Zn could be partially attributed to dissolved Zn. However, the toxicity and transcriptomic responses in all MNP treatments cannot be fully explained by dissolved Zn. Two of the biological pathways identified, one essential for protein biosynthesis (Aminoacyl-tRNA biosynthesis) and another associated with detoxification (ABC transporters), were shared among pristine and one or both transformed ZnO-MNPs, but not ZnSO4. When comparing pristine and transformed ZnO-MNPs, 66% and 40% of genes were shared between ZnO-MNPs and sZnO-MNPs or pZnO-MNPs, respectively. This suggests greater similarity in transcriptomic responses between ZnO-MNPs and sZnO-MNPs, while toxicity mechanisms are more distinct for pZnO-MNPs, where 13 unique biological pathways were identified. Based on these pathways, the toxicity of pZnO-MNPs is likely to be associated with their adverse effect on digestion and metabolism.

Authors+Show Affiliations

Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA; Department of Math and Computer Science, Belmont University, Nashville, TN, USA.Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA.Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA; State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA.Department of Math and Computer Science, Belmont University, Nashville, TN, USA; Biostatics, Epidemiology, and Research Design, Center for Clinical and Translational Science, University of Kentucky, Lexington, KY, USA.Centre for Ecology and Hydrology, Maclean Building, Benson Lane, Crowmarsh Gifford, Wallingford, Oxon, OX10 8BB, UK.Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK.Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK.Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA.Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, USA.Division of Land and Water, CSIRO, Ecosciences Precinct, Brisbane, QLD, Australia.Department of Pharmacology and Nutritional Sciences, College of Medicine, University of Kentucky, Lexington, KY, USA.Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA. Electronic address: olga.tsyusko@uky.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30823346

Citation

Starnes, Daniel, et al. "Toxicogenomic Responses of Caenorhabditis Elegans to Pristine and Transformed Zinc Oxide Nanoparticles." Environmental Pollution (Barking, Essex : 1987), vol. 247, 2019, pp. 917-926.
Starnes D, Unrine J, Chen C, et al. Toxicogenomic responses of Caenorhabditis elegans to pristine and transformed zinc oxide nanoparticles. Environ Pollut. 2019;247:917-926.
Starnes, D., Unrine, J., Chen, C., Lichtenberg, S., Starnes, C., Svendsen, C., Kille, P., Morgan, J., Baddar, Z. E., Spear, A., Bertsch, P., Chen, K. C., & Tsyusko, O. (2019). Toxicogenomic responses of Caenorhabditis elegans to pristine and transformed zinc oxide nanoparticles. Environmental Pollution (Barking, Essex : 1987), 247, 917-926. https://doi.org/10.1016/j.envpol.2019.01.077
Starnes D, et al. Toxicogenomic Responses of Caenorhabditis Elegans to Pristine and Transformed Zinc Oxide Nanoparticles. Environ Pollut. 2019;247:917-926. PubMed PMID: 30823346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxicogenomic responses of Caenorhabditis elegans to pristine and transformed zinc oxide nanoparticles. AU - Starnes,Daniel, AU - Unrine,Jason, AU - Chen,Chun, AU - Lichtenberg,Stuart, AU - Starnes,Catherine, AU - Svendsen,Claus, AU - Kille,Peter, AU - Morgan,John, AU - Baddar,Zeinah Elhaj, AU - Spear,Amanda, AU - Bertsch,Paul, AU - Chen,Kuey Chu, AU - Tsyusko,Olga, Y1 - 2019/01/29/ PY - 2018/08/21/received PY - 2018/12/20/revised PY - 2019/01/21/accepted PY - 2019/3/3/entrez PY - 2019/3/3/pubmed PY - 2019/4/9/medline KW - Gene expression KW - Nanomaterial KW - Nematode KW - Soil KW - Transcriptomics KW - Wastewater SP - 917 EP - 926 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 247 N2 - Manufactured nanoparticles (MNPs) undergo transformation immediately after they enter wastewater treatment streams and during their partitioning to sewage sludge, which is applied to agricultural soils in form of biosolids. We examined toxicogenomic responses of the model nematode Caenorhabditis elegans to pristine and transformed ZnO-MNPs (phosphatized pZnO- and sulfidized sZnO-MNPs). To account for the toxicity due to dissolved Zn, a ZnSO4 treatment was included. Transformation of ZnO-MNPs reduced their toxicity by nearly ten-fold, while there was almost no difference in the toxicity of pristine ZnO-MNPs and ZnSO4. This combined with the fact that far more dissolved Zn was released from ZnO- compared to pZnO- or sZnO-MNPs, suggests that dissolution of pristine ZnO-MNPs is one of the main drivers of their toxicity. Transcriptomic responses at the EC30 for reproduction resulted in a total of 1161 differentially expressed genes. Fifty percent of the genes differentially expressed in the ZnSO4 treatment, including the three metal responsive genes (mtl-1, mtl-2 and numr-1), were shared among all treatments, suggesting that responses to all forms of Zn could be partially attributed to dissolved Zn. However, the toxicity and transcriptomic responses in all MNP treatments cannot be fully explained by dissolved Zn. Two of the biological pathways identified, one essential for protein biosynthesis (Aminoacyl-tRNA biosynthesis) and another associated with detoxification (ABC transporters), were shared among pristine and one or both transformed ZnO-MNPs, but not ZnSO4. When comparing pristine and transformed ZnO-MNPs, 66% and 40% of genes were shared between ZnO-MNPs and sZnO-MNPs or pZnO-MNPs, respectively. This suggests greater similarity in transcriptomic responses between ZnO-MNPs and sZnO-MNPs, while toxicity mechanisms are more distinct for pZnO-MNPs, where 13 unique biological pathways were identified. Based on these pathways, the toxicity of pZnO-MNPs is likely to be associated with their adverse effect on digestion and metabolism. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/30823346/Toxicogenomic_responses_of_Caenorhabditis_elegans_to_pristine_and_transformed_zinc_oxide_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -