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Bulleyaconitine A Effectively Relieves Allergic Lung Inflammation in a Murine Asthmatic Model.
Med Sci Monit 2019; 25:1656-1662MS

Abstract

BACKGROUND

Bulleyaconitine A (BLA) has been widely used as analgesic against chronic inflammatory pain in China. However, its potential therapeutic role in asthma remains unclear. The purpose of this study was to investigate the effect of BLA on airway inflammation in mice with allergic asthma. MATERIAL AND

METHODS

Specific-pathogen-free (SPF) female Balb/c mice were randomly divided into the following 6 groups: (1) Control group (NC), (2) Asthma group (AS), (3) BLA-L group, (4) BLA-M group, (5) BLA-H group, and (6) Dexamethasone group. An asthma mouse model was established by administration of ovalbumin (OVA) and mice were sacrificed within 24 h after the last challenge. Enzyme-linked immunosorbent assay (ELISA) method was used to determine the relative expression levels of IgE and IgG in mouse serum. In addition, bronchoalveolar lavage fluid (BALF) was collected and IL-4, TNF-α, and MCP-1 levels were determined by ELISA. Furthermore, eosinophils, lymphocytes, and macrophages in BALF were classified and analyzed, and inflammatory cell infiltration in the airways of mice was determined by hematoxylin-eosin (HE) staining. The expression of NF-κB1 and PKC-δ in mouse lung tissue was determined by Western blot analysis.

RESULTS

The levels of serum IgE and IgG in BLA- or Dex- treated mice were significantly reduced compared to those in the asthma (AS) group (P<0.01), whereas the levels of cytokines IL-4, TNF-α, and MCP-1 were significantly decreased (P<0.01). HE-staining showed that BLA significantly reduced inflammatory cell infiltration and mucus secretion in lung tissue. Moreover, BLA inhibited the expression of NF-κB1 and PKC-d via the NF-κB signaling pathway in the lung.

CONCLUSIONS

Our data show that BLA activates PKC-δ/NF-κB to reduce airway inflammation in allergic asthma mice.

Authors+Show Affiliations

School of Preclinical Medicine, Wannan Medical College, Wuhu, Anhui, China (mainland).School of Preclinical Medicine, Wannan Medical College, Wuhu, Anhui, China (mainland).School of Preclinical Medicine, Wannan Medical College, Wuhu, Anhui, China (mainland).School of Preclinical Medicine, Wannan Medical College, Wuhu, Anhui, China (mainland).College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China (mainland).School of Preclinical Medicine, Wannan Medical College, Wuhu, Anhui, China (mainland).School of Preclinical Medicine, Wannan Medical College, Wuhu, Anhui, China (mainland).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30828084

Citation

Zhan, Xiaodong, et al. "Bulleyaconitine a Effectively Relieves Allergic Lung Inflammation in a Murine Asthmatic Model." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 25, 2019, pp. 1656-1662.
Zhan X, Zhang W, Sun T, et al. Bulleyaconitine A Effectively Relieves Allergic Lung Inflammation in a Murine Asthmatic Model. Med Sci Monit. 2019;25:1656-1662.
Zhan, X., Zhang, W., Sun, T., Feng, Y., Xi, Y., Jiang, Y., & Tang, X. (2019). Bulleyaconitine A Effectively Relieves Allergic Lung Inflammation in a Murine Asthmatic Model. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, pp. 1656-1662. doi:10.12659/MSM.915427.
Zhan X, et al. Bulleyaconitine a Effectively Relieves Allergic Lung Inflammation in a Murine Asthmatic Model. Med Sci Monit. 2019 Mar 4;25:1656-1662. PubMed PMID: 30828084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bulleyaconitine A Effectively Relieves Allergic Lung Inflammation in a Murine Asthmatic Model. AU - Zhan,Xiaodong, AU - Zhang,Wenqi, AU - Sun,Tian, AU - Feng,Yuling, AU - Xi,Yilong, AU - Jiang,Yuxin, AU - Tang,Xiaoniu, Y1 - 2019/03/04/ PY - 2019/3/5/entrez PY - 2019/3/5/pubmed PY - 2019/4/11/medline SP - 1656 EP - 1662 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med. Sci. Monit. VL - 25 N2 - BACKGROUND Bulleyaconitine A (BLA) has been widely used as analgesic against chronic inflammatory pain in China. However, its potential therapeutic role in asthma remains unclear. The purpose of this study was to investigate the effect of BLA on airway inflammation in mice with allergic asthma. MATERIAL AND METHODS Specific-pathogen-free (SPF) female Balb/c mice were randomly divided into the following 6 groups: (1) Control group (NC), (2) Asthma group (AS), (3) BLA-L group, (4) BLA-M group, (5) BLA-H group, and (6) Dexamethasone group. An asthma mouse model was established by administration of ovalbumin (OVA) and mice were sacrificed within 24 h after the last challenge. Enzyme-linked immunosorbent assay (ELISA) method was used to determine the relative expression levels of IgE and IgG in mouse serum. In addition, bronchoalveolar lavage fluid (BALF) was collected and IL-4, TNF-α, and MCP-1 levels were determined by ELISA. Furthermore, eosinophils, lymphocytes, and macrophages in BALF were classified and analyzed, and inflammatory cell infiltration in the airways of mice was determined by hematoxylin-eosin (HE) staining. The expression of NF-κB1 and PKC-δ in mouse lung tissue was determined by Western blot analysis. RESULTS The levels of serum IgE and IgG in BLA- or Dex- treated mice were significantly reduced compared to those in the asthma (AS) group (P<0.01), whereas the levels of cytokines IL-4, TNF-α, and MCP-1 were significantly decreased (P<0.01). HE-staining showed that BLA significantly reduced inflammatory cell infiltration and mucus secretion in lung tissue. Moreover, BLA inhibited the expression of NF-κB1 and PKC-d via the NF-κB signaling pathway in the lung. CONCLUSIONS Our data show that BLA activates PKC-δ/NF-κB to reduce airway inflammation in allergic asthma mice. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/30828084/Bulleyaconitine_A_Effectively_Relieves_Allergic_Lung_Inflammation_in_a_Murine_Asthmatic_Model L2 - https://www.medscimonit.com/download/index/idArt/915427 DB - PRIME DP - Unbound Medicine ER -