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Map-based cloning of a new total loss-of-function allele of OsHMA3 causes high cadmium accumulation in rice grain.
J Exp Bot. 2019 05 09; 70(10):2857-2871.JE

Abstract

Rice (Oryza sativa) is a major dietary source of the toxic metal cadmium (Cd). Reducing Cd transfer from soil to the rice grain is important for food safety. Rice cultivars vary widely in their Cd accumulation, but the genetic basis for this variation is not fully understood. Based on field and pot experiments comparing 26 rice cultivars, we identified a cultivar with high Cd accumulation in grain (BG367, coded as W4) and a cultivar with low grain Cd accumulation (Huajingxian 74, coded as W0). W4 showed a higher Cd translocation from roots to shoots than W0. Using chromosome single segment substitution lines derived from the two cultivars, we mapped a quantitative trait locus for Cd accumulation in grain to a 400 kb region in chromosome 7. Using yeast expression assays and transgenic complementation, we identified OsHMA3 as the causal gene at this locus. Compared with OsHMA3W0, OsHMA3W4 has a deletion of 14 amino acids predicted to be in the ATP binding domain. OsHMA3W4 showed a complete loss of transport activity for Cd in yeast assays. Taking our findings together, we have identified a new allele of OsHMA3 with a total loss-of-function, resulting in greatly elevated Cd translocation to rice shoots and grain.

Authors+Show Affiliations

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30840768

Citation

Sui, Fuqing, et al. "Map-based Cloning of a New Total Loss-of-function Allele of OsHMA3 Causes High Cadmium Accumulation in Rice Grain." Journal of Experimental Botany, vol. 70, no. 10, 2019, pp. 2857-2871.
Sui F, Zhao D, Zhu H, et al. Map-based cloning of a new total loss-of-function allele of OsHMA3 causes high cadmium accumulation in rice grain. J Exp Bot. 2019;70(10):2857-2871.
Sui, F., Zhao, D., Zhu, H., Gong, Y., Tang, Z., Huang, X. Y., Zhang, G., & Zhao, F. J. (2019). Map-based cloning of a new total loss-of-function allele of OsHMA3 causes high cadmium accumulation in rice grain. Journal of Experimental Botany, 70(10), 2857-2871. https://doi.org/10.1093/jxb/erz093
Sui F, et al. Map-based Cloning of a New Total Loss-of-function Allele of OsHMA3 Causes High Cadmium Accumulation in Rice Grain. J Exp Bot. 2019 05 9;70(10):2857-2871. PubMed PMID: 30840768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Map-based cloning of a new total loss-of-function allele of OsHMA3 causes high cadmium accumulation in rice grain. AU - Sui,Fuqing, AU - Zhao,Dikun, AU - Zhu,Haitao, AU - Gong,Yongfu, AU - Tang,Zhong, AU - Huang,Xin-Yuan, AU - Zhang,Guiquan, AU - Zhao,Fang-Jie, PY - 2018/12/23/received PY - 2019/02/12/accepted PY - 2019/3/7/pubmed PY - 2020/7/7/medline PY - 2019/3/7/entrez KW - Cadmium KW - OsHMA3 KW - food safety KW - map-based cloning KW - quantitative trait loci KW - rice KW - translocation SP - 2857 EP - 2871 JF - Journal of experimental botany JO - J Exp Bot VL - 70 IS - 10 N2 - Rice (Oryza sativa) is a major dietary source of the toxic metal cadmium (Cd). Reducing Cd transfer from soil to the rice grain is important for food safety. Rice cultivars vary widely in their Cd accumulation, but the genetic basis for this variation is not fully understood. Based on field and pot experiments comparing 26 rice cultivars, we identified a cultivar with high Cd accumulation in grain (BG367, coded as W4) and a cultivar with low grain Cd accumulation (Huajingxian 74, coded as W0). W4 showed a higher Cd translocation from roots to shoots than W0. Using chromosome single segment substitution lines derived from the two cultivars, we mapped a quantitative trait locus for Cd accumulation in grain to a 400 kb region in chromosome 7. Using yeast expression assays and transgenic complementation, we identified OsHMA3 as the causal gene at this locus. Compared with OsHMA3W0, OsHMA3W4 has a deletion of 14 amino acids predicted to be in the ATP binding domain. OsHMA3W4 showed a complete loss of transport activity for Cd in yeast assays. Taking our findings together, we have identified a new allele of OsHMA3 with a total loss-of-function, resulting in greatly elevated Cd translocation to rice shoots and grain. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/30840768/Map_based_cloning_of_a_new_total_loss_of_function_allele_of_OsHMA3_causes_high_cadmium_accumulation_in_rice_grain_ DB - PRIME DP - Unbound Medicine ER -