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Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

Abstract

Progressive muscle degeneration followed by dilated cardiomyopathy is a hallmark of muscular dystrophy. Stem cell therapy is suggested to replace diseased myofibers by healthy myofibers, although so far, we are faced by low efficiencies of migration and engraftment of stem cells. Chemokines are signalling proteins guiding cell migration and have been shown to tightly regulate muscle tissue repair. We sought to determine which chemokines are expressed in dystrophic muscles undergoing tissue remodelling. Therefore, we analysed the expression of chemokines and chemokine receptors in skeletal and cardiac muscles from Sarcoglycan-α null, Sarcoglycan-β null and immunodeficient Sgcβ-null mice. We found that several chemokines are dysregulated in dystrophic muscles. We further show that one of these, platelet-derived growth factor-B, promotes interstitial stem cell migration. This finding provides perspective to an approachable mechanism for improving stem cell homing towards dystrophic muscles.

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  • Authors+Show Affiliations

    ,

    Laboratory of Translational Cardiomyology, Department of Development and Regeneration, Stem Cell Research Institute, KU Leuven, 3000 Leuven, Belgium. jordi.camps@kuleuven.be.

    ,

    Laboratory of Translational Cardiomyology, Department of Development and Regeneration, Stem Cell Research Institute, KU Leuven, 3000 Leuven, Belgium. hanne.grosemans@kuleuven.be.

    ,

    Department of Pharmaceutical and Pharmacological Sciences, Laboratory for Viral Vector Technology and Gene Therapy, KU Leuven, 3000 Leuven, Belgium. rik.gijsbers@kuleuven.be.

    ,

    Laboratory of Skeletal Cell Biology and Physiology (SCEBP), Skeletal Biology and Engineering Research Center (SBE), KU Leuven, 3000 Leuven, Belgium. christa.maes@kuleuven.be.

    Laboratory of Translational Cardiomyology, Department of Development and Regeneration, Stem Cell Research Institute, KU Leuven, 3000 Leuven, Belgium. maurilio.sampaolesi@kuleuven.be. Department of Pharmaceutical and Pharmacological Sciences, Laboratory for Viral Vector Technology and Gene Therapy, KU Leuven, 3000 Leuven, Belgium. maurilio.sampaolesi@kuleuven.be. Laboratory of Skeletal Cell Biology and Physiology (SCEBP), Skeletal Biology and Engineering Research Center (SBE), KU Leuven, 3000 Leuven, Belgium. maurilio.sampaolesi@kuleuven.be. Human Anatomy Unit, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, 27100 Pavia, Italy. maurilio.sampaolesi@kuleuven.be.

    Source

    MeSH

    Animals
    Cell Movement
    Cells, Cultured
    Chemokines
    Humans
    Mice
    Mice, Inbred C57BL
    Muscular Dystrophies, Limb-Girdle
    Myoblasts
    Proto-Oncogene Proteins c-sis
    Receptors, Platelet-Derived Growth Factor
    Sarcoglycans

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30841538

    Citation

    Camps, Jordi, et al. "Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells." International Journal of Molecular Sciences, vol. 20, no. 5, 2019.
    Camps J, Grosemans H, Gijsbers R, et al. Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells. Int J Mol Sci. 2019;20(5).
    Camps, J., Grosemans, H., Gijsbers, R., Maes, C., & Sampaolesi, M. (2019). Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells. International Journal of Molecular Sciences, 20(5), doi:10.3390/ijms20051118.
    Camps J, et al. Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells. Int J Mol Sci. 2019 Mar 5;20(5) PubMed PMID: 30841538.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells. AU - Camps,Jordi, AU - Grosemans,Hanne, AU - Gijsbers,Rik, AU - Maes,Christa, AU - Sampaolesi,Maurilio, Y1 - 2019/03/05/ PY - 2019/01/31/received PY - 2019/02/25/revised PY - 2019/02/26/accepted PY - 2019/3/8/entrez PY - 2019/3/8/pubmed PY - 2019/6/14/medline KW - PDGF-B KW - cell migration KW - chemokines KW - limb-girdle muscular dystrophy KW - skeletal muscle KW - stem cells JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 5 N2 - Progressive muscle degeneration followed by dilated cardiomyopathy is a hallmark of muscular dystrophy. Stem cell therapy is suggested to replace diseased myofibers by healthy myofibers, although so far, we are faced by low efficiencies of migration and engraftment of stem cells. Chemokines are signalling proteins guiding cell migration and have been shown to tightly regulate muscle tissue repair. We sought to determine which chemokines are expressed in dystrophic muscles undergoing tissue remodelling. Therefore, we analysed the expression of chemokines and chemokine receptors in skeletal and cardiac muscles from Sarcoglycan-α null, Sarcoglycan-β null and immunodeficient Sgcβ-null mice. We found that several chemokines are dysregulated in dystrophic muscles. We further show that one of these, platelet-derived growth factor-B, promotes interstitial stem cell migration. This finding provides perspective to an approachable mechanism for improving stem cell homing towards dystrophic muscles. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/30841538/Growth_Factor_Screening_in_Dystrophic_Muscles_Reveals_PDGFB/PDGFRB-Mediated_Migration_of_Interstitial_Stem_Cells L2 - http://www.mdpi.com/resolver?pii=ijms20051118 DB - PRIME DP - Unbound Medicine ER -