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Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer.
J Clin Microbiol. 2019 05; 57(5)JC

Abstract

Burkholderia pseudomallei is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteremia, with mortality rates of 9% to 70%. Therefore, early identification of this organism may aid in directing appropriate management. This study aimed to use the Vitek matrix-assisted laser desorption ionization-time of flight mass spectrometer to create a spectrum for the rapid identification of B. pseudomallei Spectra from 85 isolate cultures were acquired using the Vitek mass spectrometer research mode. A SuperSpectrum was created using peak matching and subsequently activated for analysis of organism identification. All 85 isolates were correctly identified as B. pseudomallei A total of 899 spectra were analyzed and demonstrated a specificity of 99.8%. Eighty-one clinical isolates were used, of which 10 were neuromelioidosis, and no discernible spectrum difference was appreciated. Spectrum acquisition from a single spot was only successful in 374/899 (41%) of isolates. This increased to 100% when 3 spots of the same extract were analyzed. The Vitek mass spectrometer can be used for the rapid identification of B. pseudomallei with a high level of specificity.

Authors+Show Affiliations

Pathology Queensland, Townsville Hospital, Townsville, Australia ian.gassiep@gmail.com. School of Medicine, James Cook University, Brisbane, Australia.Pathology Queensland, Townsville Hospital, Townsville, Australia.Pathology Queensland, Townsville Hospital, Townsville, Australia. School of Medicine, James Cook University, Brisbane, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30842230

Citation

Gassiep, I, et al. "Identification of Burkholderia Pseudomallei By Use of the Vitek Mass Spectrometer." Journal of Clinical Microbiology, vol. 57, no. 5, 2019.
Gassiep I, Armstrong M, Norton RE. Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer. J Clin Microbiol. 2019;57(5).
Gassiep, I., Armstrong, M., & Norton, R. E. (2019). Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer. Journal of Clinical Microbiology, 57(5). https://doi.org/10.1128/JCM.00081-19
Gassiep I, Armstrong M, Norton RE. Identification of Burkholderia Pseudomallei By Use of the Vitek Mass Spectrometer. J Clin Microbiol. 2019;57(5) PubMed PMID: 30842230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer. AU - Gassiep,I, AU - Armstrong,M, AU - Norton,R E, Y1 - 2019/04/26/ PY - 2019/01/18/received PY - 2019/02/27/accepted PY - 2019/3/8/pubmed PY - 2020/7/16/medline PY - 2019/3/8/entrez KW - Burkholderia pseudomallei KW - diagnostics KW - mass spectrometer KW - matrix-assisted laser desorption ionization KW - melioidosis JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 57 IS - 5 N2 - Burkholderia pseudomallei is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteremia, with mortality rates of 9% to 70%. Therefore, early identification of this organism may aid in directing appropriate management. This study aimed to use the Vitek matrix-assisted laser desorption ionization-time of flight mass spectrometer to create a spectrum for the rapid identification of B. pseudomallei Spectra from 85 isolate cultures were acquired using the Vitek mass spectrometer research mode. A SuperSpectrum was created using peak matching and subsequently activated for analysis of organism identification. All 85 isolates were correctly identified as B. pseudomallei A total of 899 spectra were analyzed and demonstrated a specificity of 99.8%. Eighty-one clinical isolates were used, of which 10 were neuromelioidosis, and no discernible spectrum difference was appreciated. Spectrum acquisition from a single spot was only successful in 374/899 (41%) of isolates. This increased to 100% when 3 spots of the same extract were analyzed. The Vitek mass spectrometer can be used for the rapid identification of B. pseudomallei with a high level of specificity. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/30842230/Identification_of_Burkholderia_pseudomallei_by_Use_of_the_Vitek_Mass_Spectrometer_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=30842230 DB - PRIME DP - Unbound Medicine ER -