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Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice.
Plant Mol Biol. 2019 May; 100(1-2):133-149.PM

Abstract

KEY MESSAGE

The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, however its underlying molecular mechanisms remain elusive. In this study, we report a novel Oryza sativa premature leaf senescence mutant (ospls2). Through map-based cloning, a G-to-A substitution was determined at the 1st nucleotide of the 13th intron in the OsPLS2 gene that encodes a Upf1-like helicase. This mutation prompts aberrant splicing of OsPLS2 messenger and consequent disruption of its full-length protein translation, suggesting a negative role of OsPLS2 in regulating leaf senescence. Wild-type rice accordingly displayed a progressive drop of OsPSL2 protein levels with age-dependent leaf senescence. Shading and light filtration studies showed that the ospls2 phenotype, which was characteristic of photo-oxidative stress and reactive oxygen species (ROS) accumulation, was an effect of irritation by light. When continuously exposed to far-red light, exogenous H2O2 and/or abscisic acid (ABA), the ospls2 mutant sustained hypersensitive leaf senescence. In consistence, light and ROS signal pathways in ospls2 were activated by down-regulation of phytochrome genes, and up-regulation of PHYTOCHROME-INTERACTING FACTORS (PIFs) and WRKY genes, all promoting leaf senescence. Together, these data indicated that OsPLS2 played an essential role in leaf senescence and its disruption triggered light-dependent leaf senescence in rice.

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Authors+Show Affiliations

Gong P
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Luo Y
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Huang F
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Chen Y
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Zhao C
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Wu X
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Li K
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Yang X
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Cheng F
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Xiang X
Experimental Teaching Center, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.
Wu C
Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Pan G
Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China. pangang12@zju.edu.com.

MeSH

Abscisic AcidAmino Acid SequenceAntioxidantsDNA HelicasesGene Expression Regulation, PlantGenes, PlantLightMutationOryzaPhenotypePhotosynthesisPlant LeavesPlant ProteinsReactive Oxygen SpeciesTime Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30843130

Citation

Gong, Pan, et al. "Disruption of a Upf1-like Helicase-encoding Gene OsPLS2 Triggers Light-dependent Premature Leaf Senescence in Rice." Plant Molecular Biology, vol. 100, no. 1-2, 2019, pp. 133-149.
Gong P, Luo Y, Huang F, et al. Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice. Plant Mol Biol. 2019;100(1-2):133-149.
Gong, P., Luo, Y., Huang, F., Chen, Y., Zhao, C., Wu, X., Li, K., Yang, X., Cheng, F., Xiang, X., Wu, C., & Pan, G. (2019). Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice. Plant Molecular Biology, 100(1-2), 133-149. https://doi.org/10.1007/s11103-019-00848-4
Gong P, et al. Disruption of a Upf1-like Helicase-encoding Gene OsPLS2 Triggers Light-dependent Premature Leaf Senescence in Rice. Plant Mol Biol. 2019;100(1-2):133-149. PubMed PMID: 30843130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice. AU - Gong,Pan, AU - Luo,Yanmin, AU - Huang,Fudeng, AU - Chen,Yaodong, AU - Zhao,Chaoyue, AU - Wu,Xin, AU - Li,Kunyu, AU - Yang,Xi, AU - Cheng,Fangmin, AU - Xiang,Xun, AU - Wu,Chunyan, AU - Pan,Gang, Y1 - 2019/03/06/ PY - 2018/11/02/received PY - 2019/02/21/accepted PY - 2019/3/8/pubmed PY - 2019/5/28/medline PY - 2019/3/8/entrez KW - Light-dependent KW - Oryza sativa L. KW - OsPLS2 KW - Premature leaf senescence KW - ROS KW - Upf1-like helicase SP - 133 EP - 149 JF - Plant molecular biology JO - Plant Mol Biol VL - 100 IS - 1-2 N2 - KEY MESSAGE: The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, however its underlying molecular mechanisms remain elusive. In this study, we report a novel Oryza sativa premature leaf senescence mutant (ospls2). Through map-based cloning, a G-to-A substitution was determined at the 1st nucleotide of the 13th intron in the OsPLS2 gene that encodes a Upf1-like helicase. This mutation prompts aberrant splicing of OsPLS2 messenger and consequent disruption of its full-length protein translation, suggesting a negative role of OsPLS2 in regulating leaf senescence. Wild-type rice accordingly displayed a progressive drop of OsPSL2 protein levels with age-dependent leaf senescence. Shading and light filtration studies showed that the ospls2 phenotype, which was characteristic of photo-oxidative stress and reactive oxygen species (ROS) accumulation, was an effect of irritation by light. When continuously exposed to far-red light, exogenous H2O2 and/or abscisic acid (ABA), the ospls2 mutant sustained hypersensitive leaf senescence. In consistence, light and ROS signal pathways in ospls2 were activated by down-regulation of phytochrome genes, and up-regulation of PHYTOCHROME-INTERACTING FACTORS (PIFs) and WRKY genes, all promoting leaf senescence. Together, these data indicated that OsPLS2 played an essential role in leaf senescence and its disruption triggered light-dependent leaf senescence in rice. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/30843130/Disruption_of_a_Upf1_like_helicase_encoding_gene_OsPLS2_triggers_light_dependent_premature_leaf_senescence_in_rice_ DB - PRIME DP - Unbound Medicine ER -