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Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice.
Plant Mol Biol. 2019 May; 100(1-2):133-149.PM

Abstract

KEY MESSAGE

The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, however its underlying molecular mechanisms remain elusive. In this study, we report a novel Oryza sativa premature leaf senescence mutant (ospls2). Through map-based cloning, a G-to-A substitution was determined at the 1st nucleotide of the 13th intron in the OsPLS2 gene that encodes a Upf1-like helicase. This mutation prompts aberrant splicing of OsPLS2 messenger and consequent disruption of its full-length protein translation, suggesting a negative role of OsPLS2 in regulating leaf senescence. Wild-type rice accordingly displayed a progressive drop of OsPSL2 protein levels with age-dependent leaf senescence. Shading and light filtration studies showed that the ospls2 phenotype, which was characteristic of photo-oxidative stress and reactive oxygen species (ROS) accumulation, was an effect of irritation by light. When continuously exposed to far-red light, exogenous H2O2 and/or abscisic acid (ABA), the ospls2 mutant sustained hypersensitive leaf senescence. In consistence, light and ROS signal pathways in ospls2 were activated by down-regulation of phytochrome genes, and up-regulation of PHYTOCHROME-INTERACTING FACTORS (PIFs) and WRKY genes, all promoting leaf senescence. Together, these data indicated that OsPLS2 played an essential role in leaf senescence and its disruption triggered light-dependent leaf senescence in rice.

Authors+Show Affiliations

Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Experimental Teaching Center, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China.Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.Department of Agronomy, Zhejiang University, Zijingang Campus, Hangzhou, 310058, People's Republic of China. pangang12@zju.edu.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30843130

Citation

Gong, Pan, et al. "Disruption of a Upf1-like Helicase-encoding Gene OsPLS2 Triggers Light-dependent Premature Leaf Senescence in Rice." Plant Molecular Biology, vol. 100, no. 1-2, 2019, pp. 133-149.
Gong P, Luo Y, Huang F, et al. Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice. Plant Mol Biol. 2019;100(1-2):133-149.
Gong, P., Luo, Y., Huang, F., Chen, Y., Zhao, C., Wu, X., Li, K., Yang, X., Cheng, F., Xiang, X., Wu, C., & Pan, G. (2019). Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice. Plant Molecular Biology, 100(1-2), 133-149. https://doi.org/10.1007/s11103-019-00848-4
Gong P, et al. Disruption of a Upf1-like Helicase-encoding Gene OsPLS2 Triggers Light-dependent Premature Leaf Senescence in Rice. Plant Mol Biol. 2019;100(1-2):133-149. PubMed PMID: 30843130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice. AU - Gong,Pan, AU - Luo,Yanmin, AU - Huang,Fudeng, AU - Chen,Yaodong, AU - Zhao,Chaoyue, AU - Wu,Xin, AU - Li,Kunyu, AU - Yang,Xi, AU - Cheng,Fangmin, AU - Xiang,Xun, AU - Wu,Chunyan, AU - Pan,Gang, Y1 - 2019/03/06/ PY - 2018/11/02/received PY - 2019/02/21/accepted PY - 2019/3/8/pubmed PY - 2019/5/28/medline PY - 2019/3/8/entrez KW - Light-dependent KW - Oryza sativa L. KW - OsPLS2 KW - Premature leaf senescence KW - ROS KW - Upf1-like helicase SP - 133 EP - 149 JF - Plant molecular biology JO - Plant Mol Biol VL - 100 IS - 1-2 N2 - KEY MESSAGE: The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, however its underlying molecular mechanisms remain elusive. In this study, we report a novel Oryza sativa premature leaf senescence mutant (ospls2). Through map-based cloning, a G-to-A substitution was determined at the 1st nucleotide of the 13th intron in the OsPLS2 gene that encodes a Upf1-like helicase. This mutation prompts aberrant splicing of OsPLS2 messenger and consequent disruption of its full-length protein translation, suggesting a negative role of OsPLS2 in regulating leaf senescence. Wild-type rice accordingly displayed a progressive drop of OsPSL2 protein levels with age-dependent leaf senescence. Shading and light filtration studies showed that the ospls2 phenotype, which was characteristic of photo-oxidative stress and reactive oxygen species (ROS) accumulation, was an effect of irritation by light. When continuously exposed to far-red light, exogenous H2O2 and/or abscisic acid (ABA), the ospls2 mutant sustained hypersensitive leaf senescence. In consistence, light and ROS signal pathways in ospls2 were activated by down-regulation of phytochrome genes, and up-regulation of PHYTOCHROME-INTERACTING FACTORS (PIFs) and WRKY genes, all promoting leaf senescence. Together, these data indicated that OsPLS2 played an essential role in leaf senescence and its disruption triggered light-dependent leaf senescence in rice. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/30843130/Disruption_of_a_Upf1_like_helicase_encoding_gene_OsPLS2_triggers_light_dependent_premature_leaf_senescence_in_rice_ DB - PRIME DP - Unbound Medicine ER -