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Transport of Flavanolic Monomers and Procyanidin Dimer A2 across Human Adenocarcinoma Stomach Cells (MKN-28).
J Agric Food Chem. 2019 Mar 27; 67(12):3354-3362.JA

Abstract

It has been proven that A-type procyanidins, containing an additional ether bond, compared to B-type procyanidins are also bioavailable in vitro and in vivo. However, their bioavailability and absorption in the gastrointestinal tract remain uncertain. In this study, a model of the human adenocarcinoma stomach cell line (MKN-28) was established to explore the cellular transport of flavanolic monomers and procyanidin dimer A2, which were isolated from the litchi pericarp extract. After the integrity and permeability of the cell monolayer were ensured by measurement of the transepithelial electrical resistance and the apparent permeability coefficient for Lucifer yellow, the transportation of procyanidins A2 and B2, (-)-epicatechin (EC), and (+)-catechin (CC) was studied at pH 3.0, 5.0, or 7.0 in the apical side, with compound concentrations of 0.05 and 0.1 mg/mL based on the cytotoxicity test. High-performance liquid chromatography and liquid chromatography-mass spectrometry analyses indicated that EC, CC, and A2 were transported in the MKN-28 cell line from 30 to 180 min, while B2 showed no transport. The maximal transport efficiencies of EC, CC, and A2 were 23 ± 0.81, 13.16 ± 1.53, and 16.41 ± 1.36%, respectively, existing at 120, 180, and 120 min of transportation. Laser scanning confocal microscopy analysis presented the dynamic transmission of EC, in accordance with the result of concentration determination, suggesting that the A-type procyanidins are possibly absorbed through the stomach barrier, which is pH- and time-dependent.

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Publisher Full Text (DOI)

Authors

Li S
No affiliation info available
Li J
No affiliation info available
Sun Y
No affiliation info available
Huang Y
No affiliation info available
He J
No affiliation info available
Zhu Z
No affiliation info available

MeSH

AdenocarcinomaBiflavonoidsBiological AvailabilityBiological TransportCatechinCell Line, TumorChromatography, High Pressure LiquidDimerizationFlavanonesFruitGastric MucosaHumansLitchiPlant ExtractsProanthocyanidins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30848127

Citation

Li, Shuyi, et al. "Transport of Flavanolic Monomers and Procyanidin Dimer A2 Across Human Adenocarcinoma Stomach Cells (MKN-28)." Journal of Agricultural and Food Chemistry, vol. 67, no. 12, 2019, pp. 3354-3362.
Li S, Li J, Sun Y, et al. Transport of Flavanolic Monomers and Procyanidin Dimer A2 across Human Adenocarcinoma Stomach Cells (MKN-28). J Agric Food Chem. 2019;67(12):3354-3362.
Li, S., Li, J., Sun, Y., Huang, Y., He, J., & Zhu, Z. (2019). Transport of Flavanolic Monomers and Procyanidin Dimer A2 across Human Adenocarcinoma Stomach Cells (MKN-28). Journal of Agricultural and Food Chemistry, 67(12), 3354-3362. https://doi.org/10.1021/acs.jafc.9b00378
Li S, et al. Transport of Flavanolic Monomers and Procyanidin Dimer A2 Across Human Adenocarcinoma Stomach Cells (MKN-28). J Agric Food Chem. 2019 Mar 27;67(12):3354-3362. PubMed PMID: 30848127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transport of Flavanolic Monomers and Procyanidin Dimer A2 across Human Adenocarcinoma Stomach Cells (MKN-28). AU - Li,Shuyi, AU - Li,Junsheng, AU - Sun,Yiwei, AU - Huang,Yuqi, AU - He,Jingren, AU - Zhu,Zhenzhou, Y1 - 2019/03/08/ PY - 2019/3/9/pubmed PY - 2019/4/16/medline PY - 2019/3/9/entrez KW - (−)-epicatechin KW - LSCM KW - procyanidin A2 KW - stomach barrier KW - transport efficiency SP - 3354 EP - 3362 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 67 IS - 12 N2 - It has been proven that A-type procyanidins, containing an additional ether bond, compared to B-type procyanidins are also bioavailable in vitro and in vivo. However, their bioavailability and absorption in the gastrointestinal tract remain uncertain. In this study, a model of the human adenocarcinoma stomach cell line (MKN-28) was established to explore the cellular transport of flavanolic monomers and procyanidin dimer A2, which were isolated from the litchi pericarp extract. After the integrity and permeability of the cell monolayer were ensured by measurement of the transepithelial electrical resistance and the apparent permeability coefficient for Lucifer yellow, the transportation of procyanidins A2 and B2, (-)-epicatechin (EC), and (+)-catechin (CC) was studied at pH 3.0, 5.0, or 7.0 in the apical side, with compound concentrations of 0.05 and 0.1 mg/mL based on the cytotoxicity test. High-performance liquid chromatography and liquid chromatography-mass spectrometry analyses indicated that EC, CC, and A2 were transported in the MKN-28 cell line from 30 to 180 min, while B2 showed no transport. The maximal transport efficiencies of EC, CC, and A2 were 23 ± 0.81, 13.16 ± 1.53, and 16.41 ± 1.36%, respectively, existing at 120, 180, and 120 min of transportation. Laser scanning confocal microscopy analysis presented the dynamic transmission of EC, in accordance with the result of concentration determination, suggesting that the A-type procyanidins are possibly absorbed through the stomach barrier, which is pH- and time-dependent. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/30848127/Transport_of_Flavanolic_Monomers_and_Procyanidin_Dimer_A2_across_Human_Adenocarcinoma_Stomach_Cells__MKN_28__ L2 - https://doi.org/10.1021/acs.jafc.9b00378 DB - PRIME DP - Unbound Medicine ER -