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Nonylphenol can aggravate allergic rhinitis in a murine model by regulating important Th cell subtypes and their associated cytokines.
Int Immunopharmacol 2019; 70:260-267II

Abstract

Nonylphenol (NP) is a widely distributed, toxic endocrine-disrupting chemical exhibiting estrogenic activity. However, its effect on allergic rhinitis (AR) remains unclear. In this study, the effects of NP on a murine model of AR were investigated. Mice were divided into ovalbumin (OVA), NP, and control groups. OVA was used for sensitization and challenge. Mice in the NP group were administered NP during the sensitization period. Allergic nasal symptoms and eosinophil counts in nasal mucosa were measured. Serum levels of OVA-specific IgE were determined by enzyme-linked immunosorbent assay. The mRNA levels of transcription factors of Th cells were determined with real-time polymerase chain reaction. Th cell subtypes and Treg numbers were counted with the aid of multi-color flow cytometry. Cytokine concentrations in nasal mucosa were determined using the cytometric bead array method. Subcutaneous injection of NP into mice exhibiting AR enhanced not only the nasal allergic symptoms, but also eosinophil infiltration and OVA-specific IgE. Moreover, NP upregulated IL-4, IL-5, IL-13, IL-9, IL-6 and IL-17, and downregulated IL-10, in the AR mouse model; IFN-γ and IL-23 were not affected. Transcription factors and Th cell percentages were evaluated to determine whether NP regulates Th cell subtypes in an AR mouse model. GATA3, PU.1, and RORγt levels were significantly increased, but FoxP3 and Helios were decreased. In addition, Th2, Th9, and Th17 subtype percentages significantly increased, and Treg cell percentages decreased, in NP administration groups; the percentage of Th1 subtypes was not affected. NP enhanced allergic inflammation in the AR mouse model through upregulation of Th2, Th9, and Th17 responses and negative regulation of Treg responses. These results suggest that NP may be trigger AR.

Authors+Show Affiliations

Department of Medical Insurance, China Medical University Affiliated Shengjing Hospital, Shenyang City 110004, Liaoning Province, China.Department of Otorhinolaryngology, China Medical University affiliated Shengjing Hospital, Shenyang City 110004, Liaoning Province, China.Department of Otorhinolaryngology, China Medical University affiliated Shengjing Hospital, Shenyang City 110004, Liaoning Province, China.Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang City 110122, Liaoning Province, China. Electronic address: hao_liying@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30851706

Citation

Wang, Yun-Xiu, et al. "Nonylphenol Can Aggravate Allergic Rhinitis in a Murine Model By Regulating Important Th Cell Subtypes and Their Associated Cytokines." International Immunopharmacology, vol. 70, 2019, pp. 260-267.
Wang YX, Gu ZW, Cao ZW, et al. Nonylphenol can aggravate allergic rhinitis in a murine model by regulating important Th cell subtypes and their associated cytokines. Int Immunopharmacol. 2019;70:260-267.
Wang, Y. X., Gu, Z. W., Cao, Z. W., & Hao, L. Y. (2019). Nonylphenol can aggravate allergic rhinitis in a murine model by regulating important Th cell subtypes and their associated cytokines. International Immunopharmacology, 70, pp. 260-267. doi:10.1016/j.intimp.2019.02.030.
Wang YX, et al. Nonylphenol Can Aggravate Allergic Rhinitis in a Murine Model By Regulating Important Th Cell Subtypes and Their Associated Cytokines. Int Immunopharmacol. 2019;70:260-267. PubMed PMID: 30851706.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nonylphenol can aggravate allergic rhinitis in a murine model by regulating important Th cell subtypes and their associated cytokines. AU - Wang,Yun-Xiu, AU - Gu,Zhao-Wei, AU - Cao,Zhi-Wei, AU - Hao,Li-Ying, Y1 - 2019/03/06/ PY - 2018/12/09/received PY - 2019/01/30/revised PY - 2019/02/18/accepted PY - 2019/3/10/pubmed PY - 2019/8/14/medline PY - 2019/3/10/entrez KW - Allergic rhinitis KW - Helios KW - Nonylphenol KW - Th9 KW - Tregs SP - 260 EP - 267 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 70 N2 - Nonylphenol (NP) is a widely distributed, toxic endocrine-disrupting chemical exhibiting estrogenic activity. However, its effect on allergic rhinitis (AR) remains unclear. In this study, the effects of NP on a murine model of AR were investigated. Mice were divided into ovalbumin (OVA), NP, and control groups. OVA was used for sensitization and challenge. Mice in the NP group were administered NP during the sensitization period. Allergic nasal symptoms and eosinophil counts in nasal mucosa were measured. Serum levels of OVA-specific IgE were determined by enzyme-linked immunosorbent assay. The mRNA levels of transcription factors of Th cells were determined with real-time polymerase chain reaction. Th cell subtypes and Treg numbers were counted with the aid of multi-color flow cytometry. Cytokine concentrations in nasal mucosa were determined using the cytometric bead array method. Subcutaneous injection of NP into mice exhibiting AR enhanced not only the nasal allergic symptoms, but also eosinophil infiltration and OVA-specific IgE. Moreover, NP upregulated IL-4, IL-5, IL-13, IL-9, IL-6 and IL-17, and downregulated IL-10, in the AR mouse model; IFN-γ and IL-23 were not affected. Transcription factors and Th cell percentages were evaluated to determine whether NP regulates Th cell subtypes in an AR mouse model. GATA3, PU.1, and RORγt levels were significantly increased, but FoxP3 and Helios were decreased. In addition, Th2, Th9, and Th17 subtype percentages significantly increased, and Treg cell percentages decreased, in NP administration groups; the percentage of Th1 subtypes was not affected. NP enhanced allergic inflammation in the AR mouse model through upregulation of Th2, Th9, and Th17 responses and negative regulation of Treg responses. These results suggest that NP may be trigger AR. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/30851706/Nonylphenol_can_aggravate_allergic_rhinitis_in_a_murine_model_by_regulating_important_Th_cell_subtypes_and_their_associated_cytokines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(18)31283-9 DB - PRIME DP - Unbound Medicine ER -