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Multiple organ injury in male C57BL/6J mice exposed to ambient particulate matter in a real-ambient PM exposure system in Shijiazhuang, China.
Environ Pollut. 2019 May; 248:874-887.EP

Abstract

The development of a rodent ambient particulate matter (PM) inhalation system is critical for drawing causal inferences between PM exposure and the onset of human diseases. In this study, we constructed a real-ambient PM exposure system to investigate multi-organ injury and the reversibility of the impairments in C57BL/6 J male mice exposed to PM with a duration of up to three months in Shijiazhuang, a city with the highest PM2.5 concentration in China. This unique exposure system provided an optimal scenario for round-the-clock PM exposure absent a change in the physiochemical properties of PM and minimized the disturbance to the mice habitat. The mean concentration of PM2.5 in the exposure chambers was 89.95, 79.98, and 87.87 μg/m3 at three different time points, respectively: weeks 1-3, week 1-6, and week 1-12. The injury in multiple organs, including lung, brain, heart, testis, and intestine, was profound and was evident by the significant pathological and functional alterations. Pulmonary pathological examination revealed severe interstitial inflammatory and alveolar hemorrhage throughout the exposure, which was in line with the reduced lung function and the increased cytokine excretion in bronchoalveolar lavage fluid and blood plasma. Notably, the PM-mediated inflammatory response in different systems was correlated with the severity of the injury and the attenuation of pulmonary lesions in the recovery group. Thus, the PM2.5-induced inflammatory response, the chemical components-induced cytotoxicity, genetic damage, and oxidative stress might be implicated in the impairment of multiple murine organs. These findings revealed the severity, sensitivity, and reversibility of multi-organ injury in response to a real-ambient PM exposure.

Authors+Show Affiliations

Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China.Department of Toxicology, School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China.School of Public Health, Qingdao University, Qingdao, 266021, China.Department of Toxicology, School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China.Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China.Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China.School of Public Health, Qingdao University, Qingdao, 266021, China.Department of Toxicology, School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China.Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China.Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China.Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China.State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China.State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China.Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China.Persistent Organic Pollution Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.Persistent Organic Pollution Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.Department of Molecular Pharmacology, Albert Einstein College of Medicine, Forchheimer 209, 1300 Morris Park Avenue, Bronx, NY, 10461, USA.Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China.School of Public Health, Qingdao University, Qingdao, 266021, China.Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China. Electronic address: chenwen@mail.sysu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30856503

Citation

Li, Daochuan, et al. "Multiple Organ Injury in Male C57BL/6J Mice Exposed to Ambient Particulate Matter in a Real-ambient PM Exposure System in Shijiazhuang, China." Environmental Pollution (Barking, Essex : 1987), vol. 248, 2019, pp. 874-887.
Li D, Zhang R, Cui L, et al. Multiple organ injury in male C57BL/6J mice exposed to ambient particulate matter in a real-ambient PM exposure system in Shijiazhuang, China. Environ Pollut. 2019;248:874-887.
Li, D., Zhang, R., Cui, L., Chu, C., Zhang, H., Sun, H., Luo, J., Zhou, L., Chen, L., Cui, J., Chen, S., Mai, B., Chen, S., Yu, J., Cai, Z., Zhang, J., Jiang, Y., Aschner, M., Chen, R., ... Chen, W. (2019). Multiple organ injury in male C57BL/6J mice exposed to ambient particulate matter in a real-ambient PM exposure system in Shijiazhuang, China. Environmental Pollution (Barking, Essex : 1987), 248, 874-887. https://doi.org/10.1016/j.envpol.2019.02.097
Li D, et al. Multiple Organ Injury in Male C57BL/6J Mice Exposed to Ambient Particulate Matter in a Real-ambient PM Exposure System in Shijiazhuang, China. Environ Pollut. 2019;248:874-887. PubMed PMID: 30856503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple organ injury in male C57BL/6J mice exposed to ambient particulate matter in a real-ambient PM exposure system in Shijiazhuang, China. AU - Li,Daochuan, AU - Zhang,Rong, AU - Cui,Lianhua, AU - Chu,Chen, AU - Zhang,Haiyan, AU - Sun,Hao, AU - Luo,Jing, AU - Zhou,Lixiao, AU - Chen,Liping, AU - Cui,Jian, AU - Chen,Shen, AU - Mai,Bixian, AU - Chen,Shejun, AU - Yu,Jianzhen, AU - Cai,Zongwei, AU - Zhang,Jianqing, AU - Jiang,Yousheng, AU - Aschner,Michael, AU - Chen,Rui, AU - Zheng,Yuxin, AU - Chen,Wen, Y1 - 2019/03/02/ PY - 2018/11/24/received PY - 2019/02/26/revised PY - 2019/02/26/accepted PY - 2019/3/12/pubmed PY - 2019/7/10/medline PY - 2019/3/12/entrez KW - Inflammation KW - Mouse model KW - Multi-organ injury KW - Particulate matter KW - Real-ambient PM exposure system SP - 874 EP - 887 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 248 N2 - The development of a rodent ambient particulate matter (PM) inhalation system is critical for drawing causal inferences between PM exposure and the onset of human diseases. In this study, we constructed a real-ambient PM exposure system to investigate multi-organ injury and the reversibility of the impairments in C57BL/6 J male mice exposed to PM with a duration of up to three months in Shijiazhuang, a city with the highest PM2.5 concentration in China. This unique exposure system provided an optimal scenario for round-the-clock PM exposure absent a change in the physiochemical properties of PM and minimized the disturbance to the mice habitat. The mean concentration of PM2.5 in the exposure chambers was 89.95, 79.98, and 87.87 μg/m3 at three different time points, respectively: weeks 1-3, week 1-6, and week 1-12. The injury in multiple organs, including lung, brain, heart, testis, and intestine, was profound and was evident by the significant pathological and functional alterations. Pulmonary pathological examination revealed severe interstitial inflammatory and alveolar hemorrhage throughout the exposure, which was in line with the reduced lung function and the increased cytokine excretion in bronchoalveolar lavage fluid and blood plasma. Notably, the PM-mediated inflammatory response in different systems was correlated with the severity of the injury and the attenuation of pulmonary lesions in the recovery group. Thus, the PM2.5-induced inflammatory response, the chemical components-induced cytotoxicity, genetic damage, and oxidative stress might be implicated in the impairment of multiple murine organs. These findings revealed the severity, sensitivity, and reversibility of multi-organ injury in response to a real-ambient PM exposure. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/30856503/Multiple_organ_injury_in_male_C57BL/6J_mice_exposed_to_ambient_particulate_matter_in_a_real_ambient_PM_exposure_system_in_Shijiazhuang_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(18)35281-3 DB - PRIME DP - Unbound Medicine ER -