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Optimization of Phlorotannins Extraction from Fucus vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders.
Mar Drugs 2019; 17(3)MD

Abstract

Phlorotannins are phloroglucinol-based phenolic compounds, occurring particularly in brown macroalgae, that have been recognized for their promising bioactive properties. In this study, the extraction of phlorotannins from Fucus vesiculosus was evaluated with particular emphasis on the influential parameters, including the solvent concentration, solvent-solid ratio, extraction temperature and extraction time, using a single-factor design followed by a Box-Behnken design. The maximum total phlorotannin content, determined using the 2,4-dimethoxybenzaldehyde (DMBA) method, corresponded to 2.92 ± 0.05 mg of phloroglucinol equivalents/g dry seaweed (mg PGE/g DS), and was achieved for extracts carried out with acetone 67% (v/v), a solvent-solid ratio of 70 mL/g and temperature at 25 °C. This crude extract, together with a semi-purified phlorotannin fraction, were further evaluated for their anti-enzymatic capacity against α-glucosidase, α-amylase and pancreatic lipase, both showing promising inhibitory effects, particularly against α-glucosidase for which a greater inhibitory effect was observed compared to the pharmaceutical drug acarbose (IC50 = 4.5 ± 0.8 and 0.82 ± 0.3 μg/mL, respectively, against 206.6 ± 25.1 μg/mL). Additionally, the ultra-high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) analysis carried out on the ethyl acetate fraction revealed the presence of fucols, fucophlorethols, fuhalols and several other phlorotannin derivatives. Moreover, possible new phlorotannin compounds, including fucofurodiphlorethol, fucofurotriphlorethol and fucofuropentaphlorethol, have been tentatively identified in this extract. Overall, this study provides evidence that F. vesiculosus phlorotannin-rich extracts hold potential for the management of the activity of α-glucosidase, α-amylase and pancreatic lipase, which are well known to be linked to metabolic disorders such as diabetes and obesity.

Authors+Show Affiliations

QOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. mcatarino@ua.pt.QOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. artur.silva@ua.pt.REQUIMTE/LAQV, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal. nbmateus@fc.up.pt.QOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. susanacardoso@ua.pt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30857204

Citation

Catarino, Marcelo D., et al. "Optimization of Phlorotannins Extraction From Fucus Vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders." Marine Drugs, vol. 17, no. 3, 2019.
Catarino MD, Silva AMS, Mateus N, et al. Optimization of Phlorotannins Extraction from Fucus vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders. Mar Drugs. 2019;17(3).
Catarino, M. D., Silva, A. M. S., Mateus, N., & Cardoso, S. M. (2019). Optimization of Phlorotannins Extraction from Fucus vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders. Marine Drugs, 17(3), doi:10.3390/md17030162.
Catarino MD, et al. Optimization of Phlorotannins Extraction From Fucus Vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders. Mar Drugs. 2019 Mar 8;17(3) PubMed PMID: 30857204.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of Phlorotannins Extraction from Fucus vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders. AU - Catarino,Marcelo D, AU - Silva,Artur M S, AU - Mateus,Nuno, AU - Cardoso,Susana M, Y1 - 2019/03/08/ PY - 2019/01/31/received PY - 2019/02/20/revised PY - 2019/03/04/accepted PY - 2019/3/13/entrez PY - 2019/3/13/pubmed PY - 2019/6/25/medline KW - Fucus vesiculosus KW - diabetes KW - obesity KW - pancreatic lipase KW - phlorotannins KW - response surface methodology KW - α-amylase KW - α-glucosidase JF - Marine drugs JO - Mar Drugs VL - 17 IS - 3 N2 - Phlorotannins are phloroglucinol-based phenolic compounds, occurring particularly in brown macroalgae, that have been recognized for their promising bioactive properties. In this study, the extraction of phlorotannins from Fucus vesiculosus was evaluated with particular emphasis on the influential parameters, including the solvent concentration, solvent-solid ratio, extraction temperature and extraction time, using a single-factor design followed by a Box-Behnken design. The maximum total phlorotannin content, determined using the 2,4-dimethoxybenzaldehyde (DMBA) method, corresponded to 2.92 ± 0.05 mg of phloroglucinol equivalents/g dry seaweed (mg PGE/g DS), and was achieved for extracts carried out with acetone 67% (v/v), a solvent-solid ratio of 70 mL/g and temperature at 25 °C. This crude extract, together with a semi-purified phlorotannin fraction, were further evaluated for their anti-enzymatic capacity against α-glucosidase, α-amylase and pancreatic lipase, both showing promising inhibitory effects, particularly against α-glucosidase for which a greater inhibitory effect was observed compared to the pharmaceutical drug acarbose (IC50 = 4.5 ± 0.8 and 0.82 ± 0.3 μg/mL, respectively, against 206.6 ± 25.1 μg/mL). Additionally, the ultra-high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) analysis carried out on the ethyl acetate fraction revealed the presence of fucols, fucophlorethols, fuhalols and several other phlorotannin derivatives. Moreover, possible new phlorotannin compounds, including fucofurodiphlorethol, fucofurotriphlorethol and fucofuropentaphlorethol, have been tentatively identified in this extract. Overall, this study provides evidence that F. vesiculosus phlorotannin-rich extracts hold potential for the management of the activity of α-glucosidase, α-amylase and pancreatic lipase, which are well known to be linked to metabolic disorders such as diabetes and obesity. SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/30857204/Optimization_of_Phlorotannins_Extraction_from_Fucus_vesiculosus_and_Evaluation_of_Their_Potential_to_Prevent_Metabolic_Disorders_ L2 - http://www.mdpi.com/resolver?pii=md17030162 DB - PRIME DP - Unbound Medicine ER -