Tags

Type your tag names separated by a space and hit enter

Construction of an ultrasensitive electrochemiluminescent aptasensor for ractopamine detection.
Analyst. 2019 Apr 08; 144(8):2550-2555.A

Abstract

In this study, an ultrasensitive electrochemiluminescence (ECL) aptasensor based on Ru(bpy)32+-doped silica nanoparticles (Ru@SiO2 NPs) coupled with gold nanoparticles (Au NPs) was developed for the determination of ractopamine (Rac). TPrA is used as the coreactant, the Ru@SiO2 NPs serve as the ECL luminophores, and the Au NPs work as a catalyzer in the redox reaction as well as the carrier that immobilizes the aptamer. Meanwhile, the complete incorporation of the Ru@SiO2 NPs and Au NPs increases the localized surface plasmon resonance (LSPR) probability, thus promoting ECL emission. The ractopamine (Rac) target molecules are specifically captured on the electrode surface by aptamer recognition. The ECL signal is quenched by energy transfer from the luminophore to benzoquinone compounds, which are oxidative products of Rac from the electrochemical scanning process. The proposed ECL aptasensor exhibits ultrahigh sensitivity and excellent selectivity for Rac detection. The linear response ranged over Rac concentrations from 1.5 × 10-12 M to 1.5 × 10-8 M with a detection limit of 4.1 × 10-14 M (S/N = 3). The detection recovery of Rac in real meat samples confirmed the satisfactory performance of the method. This study describes a versatile ECL aptasensor based on the combined functions of luminous nanospheres and Au NPs, indicating its potential application for the ultrasensitive analysis of targets in diverse systems.

Authors+Show Affiliations

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. chen_mm@whu.edu.cn zhangxh@hubu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30865739

Citation

Xiong, Huiwen, et al. "Construction of an Ultrasensitive Electrochemiluminescent Aptasensor for Ractopamine Detection." The Analyst, vol. 144, no. 8, 2019, pp. 2550-2555.
Xiong H, Gao J, Wang Y, et al. Construction of an ultrasensitive electrochemiluminescent aptasensor for ractopamine detection. Analyst. 2019;144(8):2550-2555.
Xiong, H., Gao, J., Wang, Y., Chen, Z., Chen, M. M., Zhang, X., & Wang, S. (2019). Construction of an ultrasensitive electrochemiluminescent aptasensor for ractopamine detection. The Analyst, 144(8), 2550-2555. https://doi.org/10.1039/c9an00183b
Xiong H, et al. Construction of an Ultrasensitive Electrochemiluminescent Aptasensor for Ractopamine Detection. Analyst. 2019 Apr 8;144(8):2550-2555. PubMed PMID: 30865739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of an ultrasensitive electrochemiluminescent aptasensor for ractopamine detection. AU - Xiong,Huiwen, AU - Gao,Jingwen, AU - Wang,Ying, AU - Chen,Ziyi, AU - Chen,Miao-Miao, AU - Zhang,Xiuhua, AU - Wang,Shengfu, PY - 2019/3/14/pubmed PY - 2019/3/14/medline PY - 2019/3/14/entrez SP - 2550 EP - 2555 JF - The Analyst JO - Analyst VL - 144 IS - 8 N2 - In this study, an ultrasensitive electrochemiluminescence (ECL) aptasensor based on Ru(bpy)32+-doped silica nanoparticles (Ru@SiO2 NPs) coupled with gold nanoparticles (Au NPs) was developed for the determination of ractopamine (Rac). TPrA is used as the coreactant, the Ru@SiO2 NPs serve as the ECL luminophores, and the Au NPs work as a catalyzer in the redox reaction as well as the carrier that immobilizes the aptamer. Meanwhile, the complete incorporation of the Ru@SiO2 NPs and Au NPs increases the localized surface plasmon resonance (LSPR) probability, thus promoting ECL emission. The ractopamine (Rac) target molecules are specifically captured on the electrode surface by aptamer recognition. The ECL signal is quenched by energy transfer from the luminophore to benzoquinone compounds, which are oxidative products of Rac from the electrochemical scanning process. The proposed ECL aptasensor exhibits ultrahigh sensitivity and excellent selectivity for Rac detection. The linear response ranged over Rac concentrations from 1.5 × 10-12 M to 1.5 × 10-8 M with a detection limit of 4.1 × 10-14 M (S/N = 3). The detection recovery of Rac in real meat samples confirmed the satisfactory performance of the method. This study describes a versatile ECL aptasensor based on the combined functions of luminous nanospheres and Au NPs, indicating its potential application for the ultrasensitive analysis of targets in diverse systems. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/30865739/Construction_of_an_ultrasensitive_electrochemiluminescent_aptasensor_for_ractopamine_detection L2 - https://doi.org/10.1039/c9an00183b DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.