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The impact of sedentary work on sperm nuclear DNA integrity.
Folia Histochem Cytobiol 2019; 57(1):15-22FH

Abstract

INTRODUCTION

Contemporary professional jobs that often enforce a sedentary lifestyle and are frequently associated with testicular overheat, deserve special attention with respect to male fertility potential. Interestingly, the harmful effect of testicular heat stress on sperm characteristics including nuclear DNA integrity was well characterized; however, the influence of sedentary work on sperm chromatin has not yet been documented. Therefore, our research was designed to examine the potential effects of sedentary work not only on conventional semen features but also on sperm nuclear DNA status.

MATERIALS AND METHODS

The study was carried out on ejaculated sperm cells obtained from men who spent ≥ 50% of their time at work (≥ 17.5 h per week) in a sedentary position (n = 152) and from men who spent < 50% of their time at work in a sedentary position (n = 102). Standard semen characteristics were assessed according to the WHO 2010 recommendations, while sperm nuclear DNA fragmentation (SDF) was evaluated using the Halosperm test.

RESULTS

There were no significant differences in the standard semen parameters between the study groups. The groups differed only in SDF parameter. The men who spent at least 50% of their work time in a sedentary position had a higher proportion of SDF than the men who spent < 50% of their time at work in a sedentary position (median value 21.00% vs. 16.50%, respectively). The incidence of low SDF levels (related to 0-15% sperm cells with abnormal DNA dispersion) was significantly lower (27.63% vs. 45.10%), the percentage of men with high SDF levels (related to > 30%) was significantly higher (30.92% vs. 16.67%) in group of men who spent at least 50% of their work time in a sedentary positon. Furthermore, these men were more than twice as likely to have not a low SDF level (OR: 0.4648) and had more than twice the risk of having a high SDF level (OR: 2.2381) than the men in less sedentary occupations.

CONCLUSIONS

Despite lack of association between sedentary work and conventional semen characteristics our study revealed detrimental effect of seated work on sperm nuclear DNA integrity. A sedentary job doubled the risk of high levels of sperm DNA damage. The pathomechanism could be related to testicular heat stress resulting in sperm chromatin remodelling failure during spermiogenesis. Therefore, it seems reasonable to simultaneously carry out routine seminological analyses and tests assessing sperm chromatin status while diagnosing male infertility.

Authors+Show Affiliations

Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, Żołnierska 48, 71-210 Szczecin, Poland.Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, Żołnierska 48, 71-210 Szczecin, Poland.Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, Żołnierska 48, 71-210 Szczecin, Poland. Department of Urology and Oncological Urology, Regional Specialist Hospital in Szczecin, Arkońska 4, 71-455 Szczecin, Poland. VitroLive Fertility Clinic in Szczecin, Wojska Polskiego 103, 70-483 Szczecin, Poland.Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, Żołnierska 48, 71-210 Szczecin, Poland. VitroLive Fertility Clinic in Szczecin, Wojska Polskiego 103, 70-483 Szczecin, Poland.Department of Procreative Health, Pomeranian Medical University in Szczecin,, Żołnierska 48, 71-210 Szczecin, Poland. VitroLive Fertility Clinic in Szczecin, Wojska Polskiego 103, 70-483 Szczecin, Poland.Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland, Strzeszynska 32, 60-479 Poznan, Poland.Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland, Strzeszynska 32, 60-479 Poznan, Poland.Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, Żołnierska 48, 71-210 Szczecin, Poland. mpiasecka@ipartner.com.pl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30869154

Citation

Gill, Kamil, et al. "The Impact of Sedentary Work On Sperm Nuclear DNA Integrity." Folia Histochemica Et Cytobiologica, vol. 57, no. 1, 2019, pp. 15-22.
Gill K, Jakubik J, Kups M, et al. The impact of sedentary work on sperm nuclear DNA integrity. Folia Histochem Cytobiol. 2019;57(1):15-22.
Gill, K., Jakubik, J., Kups, M., Rosiak-Gill, A., Kurzawa, R., Kurpisz, M., ... Piasecka, M. (2019). The impact of sedentary work on sperm nuclear DNA integrity. Folia Histochemica Et Cytobiologica, 57(1), pp. 15-22. doi:10.5603/FHC.a2019.0002.
Gill K, et al. The Impact of Sedentary Work On Sperm Nuclear DNA Integrity. Folia Histochem Cytobiol. 2019;57(1):15-22. PubMed PMID: 30869154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The impact of sedentary work on sperm nuclear DNA integrity. AU - Gill,Kamil, AU - Jakubik,Joanna, AU - Kups,Michal, AU - Rosiak-Gill,Aleksandra, AU - Kurzawa,Rafal, AU - Kurpisz,Maciej, AU - Fraczek,Monika, AU - Piasecka,Malgorzata, Y1 - 2019/03/14/ PY - 2019/01/08/received PY - 2019/03/06/accepted PY - 2019/03/04/revised PY - 2019/3/15/pubmed PY - 2019/4/30/medline PY - 2019/3/15/entrez KW - DNA fragmentation KW - male reproductive health KW - sedentary work KW - sperm parameters SP - 15 EP - 22 JF - Folia histochemica et cytobiologica JO - Folia Histochem. Cytobiol. VL - 57 IS - 1 N2 - INTRODUCTION: Contemporary professional jobs that often enforce a sedentary lifestyle and are frequently associated with testicular overheat, deserve special attention with respect to male fertility potential. Interestingly, the harmful effect of testicular heat stress on sperm characteristics including nuclear DNA integrity was well characterized; however, the influence of sedentary work on sperm chromatin has not yet been documented. Therefore, our research was designed to examine the potential effects of sedentary work not only on conventional semen features but also on sperm nuclear DNA status. MATERIALS AND METHODS: The study was carried out on ejaculated sperm cells obtained from men who spent ≥ 50% of their time at work (≥ 17.5 h per week) in a sedentary position (n = 152) and from men who spent < 50% of their time at work in a sedentary position (n = 102). Standard semen characteristics were assessed according to the WHO 2010 recommendations, while sperm nuclear DNA fragmentation (SDF) was evaluated using the Halosperm test. RESULTS: There were no significant differences in the standard semen parameters between the study groups. The groups differed only in SDF parameter. The men who spent at least 50% of their work time in a sedentary position had a higher proportion of SDF than the men who spent < 50% of their time at work in a sedentary position (median value 21.00% vs. 16.50%, respectively). The incidence of low SDF levels (related to 0-15% sperm cells with abnormal DNA dispersion) was significantly lower (27.63% vs. 45.10%), the percentage of men with high SDF levels (related to > 30%) was significantly higher (30.92% vs. 16.67%) in group of men who spent at least 50% of their work time in a sedentary positon. Furthermore, these men were more than twice as likely to have not a low SDF level (OR: 0.4648) and had more than twice the risk of having a high SDF level (OR: 2.2381) than the men in less sedentary occupations. CONCLUSIONS: Despite lack of association between sedentary work and conventional semen characteristics our study revealed detrimental effect of seated work on sperm nuclear DNA integrity. A sedentary job doubled the risk of high levels of sperm DNA damage. The pathomechanism could be related to testicular heat stress resulting in sperm chromatin remodelling failure during spermiogenesis. Therefore, it seems reasonable to simultaneously carry out routine seminological analyses and tests assessing sperm chromatin status while diagnosing male infertility. SN - 1897-5631 UR - https://www.unboundmedicine.com/medline/citation/30869154/The_impact_of_sedentary_work_on_sperm_nuclear_DNA_integrity_ L2 - https://journals.viamedica.pl/folia_histochemica_cytobiologica/article/view/62562 DB - PRIME DP - Unbound Medicine ER -