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Heat-killed Bacillus sp. SJ-10 probiotic acts as a growth and humoral innate immunity response enhancer in olive flounder (Paralichthys olivaceus).
Fish Shellfish Immunol. 2019 May; 88:424-431.FS

Abstract

Investigations were carried out to evaluate and quantify the effects of dietary supplementation with heat-killed (HK) Bacillus sp. SJ-10 (BSJ-10) probiotic (1 × 108 CFU g-1) on the growth and immunity of olive flounder (Paralichthys olivaceus). Flounder (averagely 9.64 g) were divided into two groups, and fed control and HK BSJ-10 (HKBSJ-10)-inoculated diets for 8 weeks. Investigations were carried out on growth and feed utilizations, innate immunity, serum biochemical parameters, microvilli length, and pro- and anti-inflammatory cytokine gene (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, and IL-10) transcriptions. Compared to control, HKBSJ-10 diet significantly (P < 0.05) enhanced weight gain and protein efficiency ratio, 1.17 and 1.11 folds respectively. Humoral innate immune parameters, lysozyme and superoxide dismutase in treatment group were also elevated by 1.34 and 1.16 folds. Similarly, an increased (P < 0.05) relative expressions of TNF-α, IL-1β, IL-6 were recorded in liver (2.71, 3.38, and 4.12 folds respectively), and gill (2.08, 1.98, and 1.81 folds respectively) than that of controls. Moreover, after challenge with Streptococcus iniae (1 × 108 CFU mL-1), the HKBSJ-10-fed group exhibited significantly higher protection (P < 0.05) against streptococcosis compared to controls, validating the observed changes in immune parameters and induction on the cytokine-encoding genes. Therefore, HKBSJ-10 increases growth, modulates innate immune parameters, and protects olive flounders against streptococcosis.

Authors+Show Affiliations

Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea; Department of Aquaculture, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Department of Marine Bio-Materials and Aquaculture, Pukyong National University, Busan, 608-737, Republic of Korea. Electronic address: scbai@pknu.ac.kr.Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea. Electronic address: iskong@pknu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30872030

Citation

Hasan, Md Tawheed, et al. "Heat-killed Bacillus Sp. SJ-10 Probiotic Acts as a Growth and Humoral Innate Immunity Response Enhancer in Olive Flounder (Paralichthys Olivaceus)." Fish & Shellfish Immunology, vol. 88, 2019, pp. 424-431.
Hasan MT, Jang WJ, Lee BJ, et al. Heat-killed Bacillus sp. SJ-10 probiotic acts as a growth and humoral innate immunity response enhancer in olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 2019;88:424-431.
Hasan, M. T., Jang, W. J., Lee, B. J., Kim, K. W., Hur, S. W., Lim, S. G., Bai, S. C., & Kong, I. S. (2019). Heat-killed Bacillus sp. SJ-10 probiotic acts as a growth and humoral innate immunity response enhancer in olive flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 88, 424-431. https://doi.org/10.1016/j.fsi.2019.03.018
Hasan MT, et al. Heat-killed Bacillus Sp. SJ-10 Probiotic Acts as a Growth and Humoral Innate Immunity Response Enhancer in Olive Flounder (Paralichthys Olivaceus). Fish Shellfish Immunol. 2019;88:424-431. PubMed PMID: 30872030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heat-killed Bacillus sp. SJ-10 probiotic acts as a growth and humoral innate immunity response enhancer in olive flounder (Paralichthys olivaceus). AU - Hasan,Md Tawheed, AU - Jang,Won Je, AU - Lee,Bong-Joo, AU - Kim,Kang Woong, AU - Hur,Sang Woo, AU - Lim,Sang Gu, AU - Bai,Sungchul C, AU - Kong,In-Soo, Y1 - 2019/03/11/ PY - 2019/01/21/received PY - 2019/03/07/revised PY - 2019/03/08/accepted PY - 2019/3/16/pubmed PY - 2019/7/26/medline PY - 2019/3/16/entrez KW - Gene expression KW - Growth performance KW - HKBSJ-10 KW - Innate immunity KW - Olive flounder SP - 424 EP - 431 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 88 N2 - Investigations were carried out to evaluate and quantify the effects of dietary supplementation with heat-killed (HK) Bacillus sp. SJ-10 (BSJ-10) probiotic (1 × 108 CFU g-1) on the growth and immunity of olive flounder (Paralichthys olivaceus). Flounder (averagely 9.64 g) were divided into two groups, and fed control and HK BSJ-10 (HKBSJ-10)-inoculated diets for 8 weeks. Investigations were carried out on growth and feed utilizations, innate immunity, serum biochemical parameters, microvilli length, and pro- and anti-inflammatory cytokine gene (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, and IL-10) transcriptions. Compared to control, HKBSJ-10 diet significantly (P < 0.05) enhanced weight gain and protein efficiency ratio, 1.17 and 1.11 folds respectively. Humoral innate immune parameters, lysozyme and superoxide dismutase in treatment group were also elevated by 1.34 and 1.16 folds. Similarly, an increased (P < 0.05) relative expressions of TNF-α, IL-1β, IL-6 were recorded in liver (2.71, 3.38, and 4.12 folds respectively), and gill (2.08, 1.98, and 1.81 folds respectively) than that of controls. Moreover, after challenge with Streptococcus iniae (1 × 108 CFU mL-1), the HKBSJ-10-fed group exhibited significantly higher protection (P < 0.05) against streptococcosis compared to controls, validating the observed changes in immune parameters and induction on the cytokine-encoding genes. Therefore, HKBSJ-10 increases growth, modulates innate immune parameters, and protects olive flounders against streptococcosis. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/30872030/Heat_killed_Bacillus_sp__SJ_10_probiotic_acts_as_a_growth_and_humoral_innate_immunity_response_enhancer_in_olive_flounder__Paralichthys_olivaceus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(19)30166-4 DB - PRIME DP - Unbound Medicine ER -