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Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean.
Int J Mol Sci. 2019 Mar 14; 20(6)IJ

Abstract

The physiological and molecular basis of seed size formation is complex, and the development of seed coat (derived from integument cells) might be a critical factor that determines seed size formation for many endospermic seeds. Castor bean (Ricinus communis L.), a model system of studying seed biology, has large and persistent endosperm with a hard seed coat at maturity. Here, we investigated the potential molecular mechanisms underlying seed size formation in castor bean by comparing the difference between global gene expression within developing seed coat tissues between the large-seed ZB107 and small-seed ZB306. First, we observed the cell size of seed coat and concluded that the large seed coat area of ZB107 resulted from more cell numbers (rather than cell size). Furthermore, we found that the lignin proportion of seed coat was higher in ZB306. An investigation into global gene expression of developing seed coat tissues revealed that 815 genes were up-regulated and 813 were down-regulated in ZB306 relative to ZB107. Interestingly, we found that many genes involved in regulating cell division were up-regulated in ZB107, whereas many genes involved in regulating lignin biosynthesis (including several NAC members, as well as MYB46/83 and MYB58/63) and in mediating programmed cell death (such as CysEP1 and βVPE) were up-regulated in ZB306. Furthermore, the expression patterns of the genes mentioned above indicated that the lignification of seed coat tissues was enhanced and occurred earlier in the developing seeds of ZB306. Taken together, we tentatively proposed a potential scenario for explaining the molecular mechanisms of seed coat governing seed size formation in castor bean by increasing the cell number and delaying the onset of lignification in seed coat tissues in large-seed ZB107. This study not only presents new information for possible modulation of seed coat related genes to improve castor seed yield, but also provides new insights into understanding the molecular basis of seed size formation in endospermic seeds with hard seed coat.

Authors+Show Affiliations

Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yuanmin@mail.kib.ac.cn. University of the Chinese Academy of Sciences, Beijing 100049, China. yuanmin@mail.kib.ac.cn.Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. wangzaiqing@mail.kib.ac.cn. University of the Chinese Academy of Sciences, Beijing 100049, China. wangzaiqing@mail.kib.ac.cn.Jiangxi Province Key Laboratory of Oil Crops Biology, Crops Research Institute of Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China. pengzhang_123@163.com.Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. lifeia@mail.kib.ac.cn.Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China. liuaizhong@mail.kib.ac.cn.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

30875738

Citation

Yu, Anmin, et al. "Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean." International Journal of Molecular Sciences, vol. 20, no. 6, 2019.
Yu A, Wang Z, Zhang Y, et al. Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean. Int J Mol Sci. 2019;20(6).
Yu, A., Wang, Z., Zhang, Y., Li, F., & Liu, A. (2019). Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean. International Journal of Molecular Sciences, 20(6). https://doi.org/10.3390/ijms20061282
Yu A, et al. Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean. Int J Mol Sci. 2019 Mar 14;20(6) PubMed PMID: 30875738.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean. AU - Yu,Anmin, AU - Wang,Zaiqing, AU - Zhang,Yang, AU - Li,Fei, AU - Liu,Aizhong, Y1 - 2019/03/14/ PY - 2019/01/21/received PY - 2019/02/27/revised PY - 2019/02/28/accepted PY - 2019/3/17/entrez PY - 2019/3/17/pubmed PY - 2019/7/17/medline KW - castor bean KW - cell division KW - lignin KW - seed coat KW - seed size JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 6 N2 - The physiological and molecular basis of seed size formation is complex, and the development of seed coat (derived from integument cells) might be a critical factor that determines seed size formation for many endospermic seeds. Castor bean (Ricinus communis L.), a model system of studying seed biology, has large and persistent endosperm with a hard seed coat at maturity. Here, we investigated the potential molecular mechanisms underlying seed size formation in castor bean by comparing the difference between global gene expression within developing seed coat tissues between the large-seed ZB107 and small-seed ZB306. First, we observed the cell size of seed coat and concluded that the large seed coat area of ZB107 resulted from more cell numbers (rather than cell size). Furthermore, we found that the lignin proportion of seed coat was higher in ZB306. An investigation into global gene expression of developing seed coat tissues revealed that 815 genes were up-regulated and 813 were down-regulated in ZB306 relative to ZB107. Interestingly, we found that many genes involved in regulating cell division were up-regulated in ZB107, whereas many genes involved in regulating lignin biosynthesis (including several NAC members, as well as MYB46/83 and MYB58/63) and in mediating programmed cell death (such as CysEP1 and βVPE) were up-regulated in ZB306. Furthermore, the expression patterns of the genes mentioned above indicated that the lignification of seed coat tissues was enhanced and occurred earlier in the developing seeds of ZB306. Taken together, we tentatively proposed a potential scenario for explaining the molecular mechanisms of seed coat governing seed size formation in castor bean by increasing the cell number and delaying the onset of lignification in seed coat tissues in large-seed ZB107. This study not only presents new information for possible modulation of seed coat related genes to improve castor seed yield, but also provides new insights into understanding the molecular basis of seed size formation in endospermic seeds with hard seed coat. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/30875738/Global_Gene_Expression_of_Seed_Coat_Tissues_Reveals_a_Potential_Mechanism_of_Regulating_Seed_Size_Formation_in_Castor_Bean_ L2 - http://www.mdpi.com/resolver?pii=ijms20061282 DB - PRIME DP - Unbound Medicine ER -