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Endotoxemia causes increased lung tissue lipid peroxidation in unanesthetized sheep.
J Appl Physiol (1985). 1986 Jun; 60(6):2094-100.JA

Abstract

Our purpose was to determine whether lipid peroxidation of lung tissue, a reflection of O2 radical injury, occurs with endotoxin, and whether the degree of tissue change corresponds with the degree of increased protein permeability. Unanesthetized adult sheep with lung lymph fistulas were given Escherichia coli endotoxin at a dose of 2 micrograms/kg (n = 34). Tissue lipid peroxidation was measured using the thiobarbituric acid assay for malondialdehyde (MDA). The MDA content of lung tissue in nanomoles per gram increased from a control value of 48 +/- 8 to 98 +/- 18 at 5 h postendotoxin (2 micrograms/kg), whereas lung lymph protein transport (Cp), was increased 3- to 4-fold. The MDA content returned to base line with Cp by 24 h postendotoxin. Six sheep given endotoxin were pretreated with 12.5 mg/kg of ibuprofen, and six were infused with dimethylthiourea (DMTU) 0.75 g/kg. With ibuprofen, Cp was only increased 2.5- to 3-fold and MDA was increased to 69 +/- 15 nmol/g. With DMTU, the increase in Cp was comparable to that with endotoxin alone, as was the MDA of lung tissue with a value of 92 +/- 12 nmol/g. The correlation of tissue MDA with Cp in all animals was 0.83. We conclude that lipid peroxidation occurs in lung tissue after a moderately severe endotoxin injury with the degree of change corresponding to the degree of increased Cp.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3087941

Citation

Demling, R H., et al. "Endotoxemia Causes Increased Lung Tissue Lipid Peroxidation in Unanesthetized Sheep." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 60, no. 6, 1986, pp. 2094-100.
Demling RH, Lalonde C, Jin LJ, et al. Endotoxemia causes increased lung tissue lipid peroxidation in unanesthetized sheep. J Appl Physiol (1985). 1986;60(6):2094-100.
Demling, R. H., Lalonde, C., Jin, L. J., Ryan, P., & Fox, R. (1986). Endotoxemia causes increased lung tissue lipid peroxidation in unanesthetized sheep. Journal of Applied Physiology (Bethesda, Md. : 1985), 60(6), 2094-100.
Demling RH, et al. Endotoxemia Causes Increased Lung Tissue Lipid Peroxidation in Unanesthetized Sheep. J Appl Physiol (1985). 1986;60(6):2094-100. PubMed PMID: 3087941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endotoxemia causes increased lung tissue lipid peroxidation in unanesthetized sheep. AU - Demling,R H, AU - Lalonde,C, AU - Jin,L J, AU - Ryan,P, AU - Fox,R, PY - 1986/6/1/pubmed PY - 1986/6/1/medline PY - 1986/6/1/entrez SP - 2094 EP - 100 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J Appl Physiol (1985) VL - 60 IS - 6 N2 - Our purpose was to determine whether lipid peroxidation of lung tissue, a reflection of O2 radical injury, occurs with endotoxin, and whether the degree of tissue change corresponds with the degree of increased protein permeability. Unanesthetized adult sheep with lung lymph fistulas were given Escherichia coli endotoxin at a dose of 2 micrograms/kg (n = 34). Tissue lipid peroxidation was measured using the thiobarbituric acid assay for malondialdehyde (MDA). The MDA content of lung tissue in nanomoles per gram increased from a control value of 48 +/- 8 to 98 +/- 18 at 5 h postendotoxin (2 micrograms/kg), whereas lung lymph protein transport (Cp), was increased 3- to 4-fold. The MDA content returned to base line with Cp by 24 h postendotoxin. Six sheep given endotoxin were pretreated with 12.5 mg/kg of ibuprofen, and six were infused with dimethylthiourea (DMTU) 0.75 g/kg. With ibuprofen, Cp was only increased 2.5- to 3-fold and MDA was increased to 69 +/- 15 nmol/g. With DMTU, the increase in Cp was comparable to that with endotoxin alone, as was the MDA of lung tissue with a value of 92 +/- 12 nmol/g. The correlation of tissue MDA with Cp in all animals was 0.83. We conclude that lipid peroxidation occurs in lung tissue after a moderately severe endotoxin injury with the degree of change corresponding to the degree of increased Cp. SN - 8750-7587 UR - https://www.unboundmedicine.com/medline/citation/3087941/Endotoxemia_causes_increased_lung_tissue_lipid_peroxidation_in_unanesthetized_sheep_ L2 - https://journals.physiology.org/doi/10.1152/jappl.1986.60.6.2094?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -