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Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India.
Indian J Med Microbiol. 2018 Oct-Dec; 36(4):522-525.IJ

Abstract

Introduction

Coagulase Negative Staphylococci, the most commonly isolated pathogen are becoming emerging threats to the community as well as to the nosocomial environment. The present study underscores the distribution of Staphylococcal cassette chromosome mec (SCCmec) types among Methicillin resistant Coagulase Negative Staphylococci from the environmental origin.

Methods and Materials

Environmental and food sample (n = 460) from different location of northeastern region of India were collected for a period of one year and were phenotypically and genotypically screened using cefoxitin disc and PCR techniques for mecA and mecC gene detection. All the MR-CoNS isolates possessing mecA gene were subjected to 16srDNA sequencing for species identification. SCCmec typing was determined by evaluating using primer sets from type I to type V. Antibiotic susceptibility testing was performed for all the isolates. Statistical analysis with chi-square test using SPSS-21 statistical software.

Results

Methicillin resistance shown by one hundred forty three isolates were carried out for molecular analysis, among them 53.84% serves as mecA carrier. Distribution of Staphylococcus haemolyticus was more frequent and was found that SCCmec types II and V were predominant among the study isolates. Linezolid was the drug of choice for the CoNS isolates. Statistical analysis showed an insignificant result for the tested antibiotics and SCCmec types.

Conclusion

This study therefore interprets the relative importance of SCCmec types among MR-CoNS isolates.

Authors+Show Affiliations

Department of Microbiology, Assam University, Silchar, Assam, India.Department of Microbiology, Assam University, Silchar, Assam, India.Department of Microbiology, Assam University, Silchar, Assam, India.Department of Microbiology, Assam University, Silchar, Assam, India.Department of Microbiology, Assam University, Silchar, Assam, India.Department of Microbiology, Assam University, Silchar, Assam, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30880700

Citation

Bhowmik, Deepshikha, et al. "Occurrence of II and V Staphylococcal Cassette Chromosome Mec Types Among Coagulase-negative Staphylococci From Northeastern Part of India." Indian Journal of Medical Microbiology, vol. 36, no. 4, 2018, pp. 522-525.
Bhowmik D, Chakraborty P, Bhowmick R, et al. Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India. Indian J Med Microbiol. 2018;36(4):522-525.
Bhowmik, D., Chakraborty, P., Bhowmick, R., Sarkar, R., Chetri, S., & Bhattacharjee, A. (2018). Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India. Indian Journal of Medical Microbiology, 36(4), 522-525. https://doi.org/10.4103/ijmm.IJMM_18_285
Bhowmik D, et al. Occurrence of II and V Staphylococcal Cassette Chromosome Mec Types Among Coagulase-negative Staphylococci From Northeastern Part of India. Indian J Med Microbiol. 2018 Oct-Dec;36(4):522-525. PubMed PMID: 30880700.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India. AU - Bhowmik,Deepshikha, AU - Chakraborty,Pinki, AU - Bhowmick,Rituparna, AU - Sarkar,Rakesh, AU - Chetri,Shiela, AU - Bhattacharjee,Amitabha, PY - 2019/3/19/entrez PY - 2019/3/19/pubmed PY - 2019/8/6/medline KW - Methicillin-resistant coagulase-negative staphylococci KW - SPSS-21 KW - mec genes KW - staphylococcal cassette chromosome mec SP - 522 EP - 525 JF - Indian journal of medical microbiology JO - Indian J Med Microbiol VL - 36 IS - 4 N2 - Introduction: Coagulase Negative Staphylococci, the most commonly isolated pathogen are becoming emerging threats to the community as well as to the nosocomial environment. The present study underscores the distribution of Staphylococcal cassette chromosome mec (SCCmec) types among Methicillin resistant Coagulase Negative Staphylococci from the environmental origin. Methods and Materials: Environmental and food sample (n = 460) from different location of northeastern region of India were collected for a period of one year and were phenotypically and genotypically screened using cefoxitin disc and PCR techniques for mecA and mecC gene detection. All the MR-CoNS isolates possessing mecA gene were subjected to 16srDNA sequencing for species identification. SCCmec typing was determined by evaluating using primer sets from type I to type V. Antibiotic susceptibility testing was performed for all the isolates. Statistical analysis with chi-square test using SPSS-21 statistical software. Results: Methicillin resistance shown by one hundred forty three isolates were carried out for molecular analysis, among them 53.84% serves as mecA carrier. Distribution of Staphylococcus haemolyticus was more frequent and was found that SCCmec types II and V were predominant among the study isolates. Linezolid was the drug of choice for the CoNS isolates. Statistical analysis showed an insignificant result for the tested antibiotics and SCCmec types. Conclusion: This study therefore interprets the relative importance of SCCmec types among MR-CoNS isolates. SN - 1998-3646 UR - https://www.unboundmedicine.com/medline/citation/30880700/Occurrence_of_II_and_V_staphylococcal_cassette_chromosome_mec_types_among_coagulase_negative_staphylococci_from_Northeastern_part_of_India_ L2 - https://linkinghub.elsevier.com/retrieve/pii/IndianJMedMicrobiol_2018_36_4_522_254391 DB - PRIME DP - Unbound Medicine ER -