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All human monocytes have the capability of expressing HLA-DQ and HLA-DP molecules upon stimulation with interferon-gamma.
J Immunol. 1986 Jul 15; 137(2):519-24.JI

Abstract

We have simultaneously studied expression of all three classes of human Ia (HLA-DR, DP, and DQ) on normal human B cells and monocytes (M phi) by using two-color immunofluorescence and flow cytometry. Expression was investigated on freshly isolated cells and after incubation of cells for 48 and 96 hr in interferon-gamma (IFN-gamma). All freshly isolated B cells express high levels of DR, DQ, and DP, and these levels are unchanged by incubation with IFN-gamma for 48 hr and 96 hr. In contrast, freshly isolated M phi are on the average 91% DR+, 32% DQ+, and 15% DP+. Incubation with IFN-gamma increases Ia expression on M phi to 98% DR+, 75% DQ+, and 58% DP+ at 48 hr, with virtually all cells becoming positive for all three Ia antigens at 96 hr. Furthermore, after the 96-hr incubation, antigen density increases 10-fold for DR, 15-fold for DQ, and 15-fold for DP in M phi to reach levels of expression comparable with B cells. These studies demonstrate that all peripheral blood monocytes have the capacity to become HLA-DQ and HLA-DP positive; IFN-gamma regulates expression of all three classes of human Ia in M phi; and IFN-gamma does not significantly modulate Ia expression in B cells.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

3088102

Citation

Gonwa, T A., et al. "All Human Monocytes Have the Capability of Expressing HLA-DQ and HLA-DP Molecules Upon Stimulation With Interferon-gamma." Journal of Immunology (Baltimore, Md. : 1950), vol. 137, no. 2, 1986, pp. 519-24.
Gonwa TA, Frost JP, Karr RW. All human monocytes have the capability of expressing HLA-DQ and HLA-DP molecules upon stimulation with interferon-gamma. J Immunol. 1986;137(2):519-24.
Gonwa, T. A., Frost, J. P., & Karr, R. W. (1986). All human monocytes have the capability of expressing HLA-DQ and HLA-DP molecules upon stimulation with interferon-gamma. Journal of Immunology (Baltimore, Md. : 1950), 137(2), 519-24.
Gonwa TA, Frost JP, Karr RW. All Human Monocytes Have the Capability of Expressing HLA-DQ and HLA-DP Molecules Upon Stimulation With Interferon-gamma. J Immunol. 1986 Jul 15;137(2):519-24. PubMed PMID: 3088102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - All human monocytes have the capability of expressing HLA-DQ and HLA-DP molecules upon stimulation with interferon-gamma. AU - Gonwa,T A, AU - Frost,J P, AU - Karr,R W, PY - 1986/7/15/pubmed PY - 1986/7/15/medline PY - 1986/7/15/entrez SP - 519 EP - 24 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 137 IS - 2 N2 - We have simultaneously studied expression of all three classes of human Ia (HLA-DR, DP, and DQ) on normal human B cells and monocytes (M phi) by using two-color immunofluorescence and flow cytometry. Expression was investigated on freshly isolated cells and after incubation of cells for 48 and 96 hr in interferon-gamma (IFN-gamma). All freshly isolated B cells express high levels of DR, DQ, and DP, and these levels are unchanged by incubation with IFN-gamma for 48 hr and 96 hr. In contrast, freshly isolated M phi are on the average 91% DR+, 32% DQ+, and 15% DP+. Incubation with IFN-gamma increases Ia expression on M phi to 98% DR+, 75% DQ+, and 58% DP+ at 48 hr, with virtually all cells becoming positive for all three Ia antigens at 96 hr. Furthermore, after the 96-hr incubation, antigen density increases 10-fold for DR, 15-fold for DQ, and 15-fold for DP in M phi to reach levels of expression comparable with B cells. These studies demonstrate that all peripheral blood monocytes have the capacity to become HLA-DQ and HLA-DP positive; IFN-gamma regulates expression of all three classes of human Ia in M phi; and IFN-gamma does not significantly modulate Ia expression in B cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/3088102/All_human_monocytes_have_the_capability_of_expressing_HLA_DQ_and_HLA_DP_molecules_upon_stimulation_with_interferon_gamma_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=3088102 DB - PRIME DP - Unbound Medicine ER -