Tags

Type your tag names separated by a space and hit enter

Genome-wide association analyses for yield and yield-related traits in bread wheat (Triticum aestivum L.) under pre-anthesis combined heat and drought stress in field conditions.
PLoS One. 2019; 14(3):e0213407.Plos

Abstract

Understanding the genetic basis of heat and drought stress tolerance in wheat is prerequisite for wheat breeding program. In the present study, a wheat panel comprising of 192 elite bread wheat genotypes was phenotyped in eight environments for yield and related traits in field conditions. Four stress environments were created by implying four different treatments differing in sowing date and water availability, panel was evaluated for two years in field conditions. The panel was genotyped with 15K Illumina chip and 9236 polymorphic markers concentrated on B genome were employed in GWAS analysis. Consistent, fast LD decay was observed on D genome and structure analysis germplasm divided panel into three major populations. GWAS was performed using BLUEs values of combined environment data in R package GAPIT using log10(P) = 3.96 as significance threshold. The significance of association was further checked using FDR<0.05 threshold. The GWAS identified 487 loci associated with the traits and were significant at log10(p) threshold out of these 350 loci were significant at FDR threshold. For two stress indices 108 associations were significant at FDR threshold. Nine genomic regions were shared among all treatment, while multiple pleiotropic regions were present on chromosome 7D followed by unmapped chromosome. The present study validated many marker trait associations for yield and other traits, MTAs significant under combined drought and heat stress were novel. These regions are important and can be used for fine mapping and marker assisted selection to discover new genes responsible for heat and drought tolerance in wheat.

Authors+Show Affiliations

Department of Botany, PMAS- Arid Agriculture University, Rawalpindi, Pakistan.Department of Botany, PMAS- Arid Agriculture University, Rawalpindi, Pakistan.Department of Biosciences, COMSATS University, Pakistan.Department of Agriculture, Hazara University Dhodial, Mansehra, Khyber Pakhtunkhwa, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30883588

Citation

Qaseem, Mirza Faisal, et al. "Genome-wide Association Analyses for Yield and Yield-related Traits in Bread Wheat (Triticum Aestivum L.) Under Pre-anthesis Combined Heat and Drought Stress in Field Conditions." PloS One, vol. 14, no. 3, 2019, pp. e0213407.
Qaseem MF, Qureshi R, Shaheen H, et al. Genome-wide association analyses for yield and yield-related traits in bread wheat (Triticum aestivum L.) under pre-anthesis combined heat and drought stress in field conditions. PLoS One. 2019;14(3):e0213407.
Qaseem, M. F., Qureshi, R., Shaheen, H., & Shafqat, N. (2019). Genome-wide association analyses for yield and yield-related traits in bread wheat (Triticum aestivum L.) under pre-anthesis combined heat and drought stress in field conditions. PloS One, 14(3), e0213407. https://doi.org/10.1371/journal.pone.0213407
Qaseem MF, et al. Genome-wide Association Analyses for Yield and Yield-related Traits in Bread Wheat (Triticum Aestivum L.) Under Pre-anthesis Combined Heat and Drought Stress in Field Conditions. PLoS One. 2019;14(3):e0213407. PubMed PMID: 30883588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide association analyses for yield and yield-related traits in bread wheat (Triticum aestivum L.) under pre-anthesis combined heat and drought stress in field conditions. AU - Qaseem,Mirza Faisal, AU - Qureshi,Rahmatullah, AU - Shaheen,Humaira, AU - Shafqat,Noshin, Y1 - 2019/03/18/ PY - 2018/12/14/received PY - 2019/02/20/accepted PY - 2019/3/19/entrez PY - 2019/3/19/pubmed PY - 2019/12/18/medline SP - e0213407 EP - e0213407 JF - PloS one JO - PLoS One VL - 14 IS - 3 N2 - Understanding the genetic basis of heat and drought stress tolerance in wheat is prerequisite for wheat breeding program. In the present study, a wheat panel comprising of 192 elite bread wheat genotypes was phenotyped in eight environments for yield and related traits in field conditions. Four stress environments were created by implying four different treatments differing in sowing date and water availability, panel was evaluated for two years in field conditions. The panel was genotyped with 15K Illumina chip and 9236 polymorphic markers concentrated on B genome were employed in GWAS analysis. Consistent, fast LD decay was observed on D genome and structure analysis germplasm divided panel into three major populations. GWAS was performed using BLUEs values of combined environment data in R package GAPIT using log10(P) = 3.96 as significance threshold. The significance of association was further checked using FDR<0.05 threshold. The GWAS identified 487 loci associated with the traits and were significant at log10(p) threshold out of these 350 loci were significant at FDR threshold. For two stress indices 108 associations were significant at FDR threshold. Nine genomic regions were shared among all treatment, while multiple pleiotropic regions were present on chromosome 7D followed by unmapped chromosome. The present study validated many marker trait associations for yield and other traits, MTAs significant under combined drought and heat stress were novel. These regions are important and can be used for fine mapping and marker assisted selection to discover new genes responsible for heat and drought tolerance in wheat. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/30883588/Genome_wide_association_analyses_for_yield_and_yield_related_traits_in_bread_wheat__Triticum_aestivum_L___under_pre_anthesis_combined_heat_and_drought_stress_in_field_conditions_ L2 - https://dx.plos.org/10.1371/journal.pone.0213407 DB - PRIME DP - Unbound Medicine ER -