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Osmotic stress induces biofilm production by Staphylococcus epidermidis isolates from neonates.

Abstract

Staphylococcus epidermidis is one of the leading causes of bloodstream infections, particularly in premature neonates, and biofilm formation is a major virulence factor. We characterized biofilm formation by 50 S. epidermidis neonatal isolates under osmotic stress and evaluated the expression of biofilm-associated genes. Phenotypical analyses of biofilm production were performed in culture medium with or without addition of NaCl or glucose. In control medium (no additions), most isolates (84%) were nonproducers or weak biofilm producers. Growth in NaCl-containing medium increased the number of moderate/strong producers, and this increase was even greater in medium containing glucose. Most of the protein-enriched biofilms (60%) could be observed only during growth in glucose, whereas 50% of the polysaccharide-enriched biofilms were observed during growth in NaCl. Studies that evaluate the conditions used to characterize biofilm production are important to help us understand the dynamics of this important virulence factor in S. epidermidis and their impact on neonatal infections.

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  • Authors+Show Affiliations

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    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: rferreira@micro.ufrj.br.

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    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: marcellec.ferreira@yahoo.com.br.

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    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: glatthardt.thais@gmail.com.

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    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: myllenapsss@gmail.com.

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    Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro, Brazil. Electronic address: raianee@gmail.com.

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    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: vivi.salgado@gmail.com.

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    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: lorraynecardog@yahoo.com.br.

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    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: eduardoalvesufrj@gmail.com.

    Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: santoskrn@micro.ufrj.br.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30885396

    Citation

    Ferreira, Rosana B R., et al. "Osmotic Stress Induces Biofilm Production By Staphylococcus Epidermidis Isolates From Neonates." Diagnostic Microbiology and Infectious Disease, vol. 94, no. 4, 2019, pp. 337-341.
    Ferreira RBR, Ferreira MCS, Glatthardt T, et al. Osmotic stress induces biofilm production by Staphylococcus epidermidis isolates from neonates. Diagn Microbiol Infect Dis. 2019;94(4):337-341.
    Ferreira, R. B. R., Ferreira, M. C. S., Glatthardt, T., Silvério, M. P., Chamon, R. C., Salgueiro, V. C., ... Dos Santos, K. R. N. (2019). Osmotic stress induces biofilm production by Staphylococcus epidermidis isolates from neonates. Diagnostic Microbiology and Infectious Disease, 94(4), pp. 337-341. doi:10.1016/j.diagmicrobio.2019.02.009.
    Ferreira RBR, et al. Osmotic Stress Induces Biofilm Production By Staphylococcus Epidermidis Isolates From Neonates. Diagn Microbiol Infect Dis. 2019;94(4):337-341. PubMed PMID: 30885396.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Osmotic stress induces biofilm production by Staphylococcus epidermidis isolates from neonates. AU - Ferreira,Rosana B R, AU - Ferreira,Marcelle C S, AU - Glatthardt,Thaís, AU - Silvério,Myllena Pereira, AU - Chamon,Raiane C, AU - Salgueiro,Vivian C, AU - Guimarães,Lorrayne Cardoso, AU - Alves,Eduardo Souza, AU - Dos Santos,Kátia R N, Y1 - 2019/02/20/ PY - 2018/10/30/received PY - 2019/01/30/revised PY - 2019/02/09/accepted PY - 2019/3/20/pubmed PY - 2019/3/20/medline PY - 2019/3/20/entrez KW - Biofilm production KW - Bloodstream infections KW - Nasal colonization KW - Neonates KW - Staphylococcus epidermidis KW - aap KW - ica SP - 337 EP - 341 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 94 IS - 4 N2 - Staphylococcus epidermidis is one of the leading causes of bloodstream infections, particularly in premature neonates, and biofilm formation is a major virulence factor. We characterized biofilm formation by 50 S. epidermidis neonatal isolates under osmotic stress and evaluated the expression of biofilm-associated genes. Phenotypical analyses of biofilm production were performed in culture medium with or without addition of NaCl or glucose. In control medium (no additions), most isolates (84%) were nonproducers or weak biofilm producers. Growth in NaCl-containing medium increased the number of moderate/strong producers, and this increase was even greater in medium containing glucose. Most of the protein-enriched biofilms (60%) could be observed only during growth in glucose, whereas 50% of the polysaccharide-enriched biofilms were observed during growth in NaCl. Studies that evaluate the conditions used to characterize biofilm production are important to help us understand the dynamics of this important virulence factor in S. epidermidis and their impact on neonatal infections. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/30885396/Osmotic_stress_induces_biofilm_production_by_Staphylococcus_epidermidis_isolates_from_neonates L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(18)30577-7 DB - PRIME DP - Unbound Medicine ER -