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The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System.
Front Immunol 2019; 10:246FI

Abstract

In the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim was to develop a tool that would be universal for all lymphoid PIDs and offer high sensitivity to identify a lymphoid PID (without a need for specificity to diagnose particular PID) and to guide and prioritize further diagnostic modalities and clinical management. The tube composition has been defined in a stepwise manner through several cycles of design-testing-evaluation-redesign in a multicenter setting. Equally important appeared to be the standardized pre-analytical procedures (sample preparation and instrument setup), analytical procedures (immunostaining and data acquisition), the software analysis (a multidimensional view based on a reference database in Infinicyt software), and data interpretation. This standardized EuroFlow concept has been tested on 250 healthy controls and 99 PID patients with defined genetic defects. In addition, an application of new EuroFlow software tools with multidimensional pattern recognition was designed with inclusion of maturation pathways in multidimensional patterns (APS plots). The major advantage of the EuroFlow approach is that data can be fully exchanged between different laboratories in any country of the world, which is especially of interest for the PID field, with generally low numbers of cases per center.

Authors+Show Affiliations

Department of Immunology, Erasmus MC, Rotterdam, Netherlands. Department of Pediatrics, Laboratory for Immunology, Leiden University Medical Center, Leiden, Netherlands.Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czechia.Department of Medicine, Cancer Research Centre (IBMCC, USAL-CSIC), Cytometry Service (NUCLEUS), University of Salamanca (USAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain. Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), Instituto de Salud Carlos III, Madrid, Spain.Institute of Clinical Immunology and Allergology, St Anne's University Hospital, Brno, Czechia.Immunology, Universitario La Paz, Madrid, Spain.Department of Medicine, Cancer Research Centre (IBMCC, USAL-CSIC), Cytometry Service (NUCLEUS), University of Salamanca (USAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain. Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), Instituto de Salud Carlos III, Madrid, Spain.Laboratory for Clinical Biology and Hematology, University Hospital Ghent, Ghent, Belgium.Faculdade de Medicina, Instituto de Medicina Molecular, Universidade de Lisboa, Lisbon, Portugal.BRC-Translational Immunology Lab, University of Oxford, Oxford, United Kingdom.Department of Immunology, Erasmus MC, Rotterdam, Netherlands.Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czechia.Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czechia.Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czechia.Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, VIC, Australia.Department of Medicine, Cancer Research Centre (IBMCC, USAL-CSIC), Cytometry Service (NUCLEUS), University of Salamanca (USAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain. Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), Instituto de Salud Carlos III, Madrid, Spain.Department of Immunology, Erasmus MC, Rotterdam, Netherlands. Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30886612

Citation

van der Burg, Mirjam, et al. "The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System." Frontiers in Immunology, vol. 10, 2019, p. 246.
van der Burg M, Kalina T, Perez-Andres M, et al. The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System. Front Immunol. 2019;10:246.
van der Burg, M., Kalina, T., Perez-Andres, M., Vlkova, M., Lopez-Granados, E., Blanco, E., ... van Dongen, J. J. M. (2019). The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System. Frontiers in Immunology, 10, p. 246. doi:10.3389/fimmu.2019.00246.
van der Burg M, et al. The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System. Front Immunol. 2019;10:246. PubMed PMID: 30886612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System. AU - van der Burg,Mirjam, AU - Kalina,Tomas, AU - Perez-Andres,Martin, AU - Vlkova,Marcela, AU - Lopez-Granados,Eduardo, AU - Blanco,Elena, AU - Bonroy,Carolien, AU - Sousa,Ana E, AU - Kienzler,Anne-Kathrin, AU - Wentink,Marjolein, AU - Mejstríková,Ester, AU - Šinkorova,Vendula, AU - Stuchly,Jan, AU - van Zelm,Menno C, AU - Orfao,Alberto, AU - van Dongen,Jacques J M, Y1 - 2019/03/04/ PY - 2018/12/05/received PY - 2019/01/29/accepted PY - 2019/3/20/entrez PY - 2019/3/20/pubmed PY - 2019/3/20/medline KW - EuroFlow KW - automated gating strategy KW - flow cytometric immunophenotyping KW - primary immunodeficiencies KW - standardization SP - 246 EP - 246 JF - Frontiers in immunology JO - Front Immunol VL - 10 N2 - In the rapidly evolving field of primary immunodeficiencies (PID), the EuroFlow consortium decided to develop a PID orientation and screening tube that facilitates fast, standardized, and validated immunophenotypic diagnosis of lymphoid PID, and allows full exchange of data between centers. Our aim was to develop a tool that would be universal for all lymphoid PIDs and offer high sensitivity to identify a lymphoid PID (without a need for specificity to diagnose particular PID) and to guide and prioritize further diagnostic modalities and clinical management. The tube composition has been defined in a stepwise manner through several cycles of design-testing-evaluation-redesign in a multicenter setting. Equally important appeared to be the standardized pre-analytical procedures (sample preparation and instrument setup), analytical procedures (immunostaining and data acquisition), the software analysis (a multidimensional view based on a reference database in Infinicyt software), and data interpretation. This standardized EuroFlow concept has been tested on 250 healthy controls and 99 PID patients with defined genetic defects. In addition, an application of new EuroFlow software tools with multidimensional pattern recognition was designed with inclusion of maturation pathways in multidimensional patterns (APS plots). The major advantage of the EuroFlow approach is that data can be fully exchanged between different laboratories in any country of the world, which is especially of interest for the PID field, with generally low numbers of cases per center. SN - 1664-3224 UR - https://www.unboundmedicine.com/medline/citation/30886612/The_EuroFlow_PID_Orientation_Tube_for_Flow_Cytometric_Diagnostic_Screening_of_Primary_Immunodeficiencies_of_the_Lymphoid_System L2 - https://doi.org/10.3389/fimmu.2019.00246 DB - PRIME DP - Unbound Medicine ER -