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Development of 3D printable conductive hydrogel with crystallized PEDOT:PSS for neural tissue engineering.

Abstract

Bioelectronic devices enable efficient and effective communication between medical devices and human tissue in order to directly treat patients with various neurological disorders. Due to the mechanical similarity to human tissue, hydrogel-based electronic devices are considered to be promising for biological signal recording and stimulation of living tissues. Here, we report the first three-dimensionally (3D) printable conductive hydrogel that can be photocrosslinked while retaining high electrical conductivity. In addition, we prepared dorsal root ganglion (DRG) cell-encapsulated gelatin methacryloyl (GelMA) hydrogels which were integrated with the 3D printed conductive structure and evaluated for efficiency neural differentiation under electrical stimulation (ES). For enhanced electrical conductivity, a poly(3,4-ethylenedioxythiophene) (PEDOT): polystyrene sulfonate (PSS) aqueous solution was freeze-dried and mixed with polyethylene glycol diacrylate (PEGDA) as the photocurable polymer base. Next, the conductive hydrogel was patterned on the substrate by using a table-top stereolithography (SLA) 3D printer. The fabricated hydrogel was characterized for electrochemical conductivity. After printing with the PEDOT:PSS conductive solution, the patterned hydrogel exhibited decreased printing diameters with increasing of PEDOT:PSS concentration. Also, the resultant conductive hydrogel had significantly increased electrochemical properties with increasing PEDOT:PSS concentration. The 3D printed conductive hydrogel provides excellent structural support to systematically transfer the ES toward encapsulated DRG cells for enhanced neuronal differentiation. The results from this study indicate that the conductive hydrogel can be useful as a 3D printing material for electrical applications.

Authors+Show Affiliations

Department of Mechanical and Aerospace Engineering, The George Washington University, DC 20052, USA.Department of Mechanical and Aerospace Engineering, The George Washington University, DC 20052, USA.Department of Physics, The George Washington University, DC 20052, USA.Department of Physics, The George Washington University, DC 20052, USA.Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland 4059, Australia.Department of Mechanical and Aerospace Engineering, The George Washington University, DC 20052, USA; Department of Biomedical Engineering, The George Washington University, DC 20052, USA; Department of Medicine, The George Washington University, DC 20052, USA. Electronic address: lgzhang@gwu.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30889733

Citation

Heo, Dong Nyoung, et al. "Development of 3D Printable Conductive Hydrogel With Crystallized PEDOT:PSS for Neural Tissue Engineering." Materials Science & Engineering. C, Materials for Biological Applications, vol. 99, 2019, pp. 582-590.
Heo DN, Lee SJ, Timsina R, et al. Development of 3D printable conductive hydrogel with crystallized PEDOT:PSS for neural tissue engineering. Mater Sci Eng C Mater Biol Appl. 2019;99:582-590.
Heo, D. N., Lee, S. J., Timsina, R., Qiu, X., Castro, N. J., & Zhang, L. G. (2019). Development of 3D printable conductive hydrogel with crystallized PEDOT:PSS for neural tissue engineering. Materials Science & Engineering. C, Materials for Biological Applications, 99, pp. 582-590. doi:10.1016/j.msec.2019.02.008.
Heo DN, et al. Development of 3D Printable Conductive Hydrogel With Crystallized PEDOT:PSS for Neural Tissue Engineering. Mater Sci Eng C Mater Biol Appl. 2019;99:582-590. PubMed PMID: 30889733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of 3D printable conductive hydrogel with crystallized PEDOT:PSS for neural tissue engineering. AU - Heo,Dong Nyoung, AU - Lee,Se-Jun, AU - Timsina,Raju, AU - Qiu,Xiangyun, AU - Castro,Nathan J, AU - Zhang,Lijie Grace, Y1 - 2019/02/02/ PY - 2018/09/24/received PY - 2019/01/24/revised PY - 2019/02/01/accepted PY - 2019/3/21/entrez PY - 2019/3/21/pubmed PY - 2019/7/11/medline KW - 3D printing KW - Conductive polymer KW - Electrical stimulation KW - Neurogenic differentiation KW - Photocurable hydrogel SP - 582 EP - 590 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 99 N2 - Bioelectronic devices enable efficient and effective communication between medical devices and human tissue in order to directly treat patients with various neurological disorders. Due to the mechanical similarity to human tissue, hydrogel-based electronic devices are considered to be promising for biological signal recording and stimulation of living tissues. Here, we report the first three-dimensionally (3D) printable conductive hydrogel that can be photocrosslinked while retaining high electrical conductivity. In addition, we prepared dorsal root ganglion (DRG) cell-encapsulated gelatin methacryloyl (GelMA) hydrogels which were integrated with the 3D printed conductive structure and evaluated for efficiency neural differentiation under electrical stimulation (ES). For enhanced electrical conductivity, a poly(3,4-ethylenedioxythiophene) (PEDOT): polystyrene sulfonate (PSS) aqueous solution was freeze-dried and mixed with polyethylene glycol diacrylate (PEGDA) as the photocurable polymer base. Next, the conductive hydrogel was patterned on the substrate by using a table-top stereolithography (SLA) 3D printer. The fabricated hydrogel was characterized for electrochemical conductivity. After printing with the PEDOT:PSS conductive solution, the patterned hydrogel exhibited decreased printing diameters with increasing of PEDOT:PSS concentration. Also, the resultant conductive hydrogel had significantly increased electrochemical properties with increasing PEDOT:PSS concentration. The 3D printed conductive hydrogel provides excellent structural support to systematically transfer the ES toward encapsulated DRG cells for enhanced neuronal differentiation. The results from this study indicate that the conductive hydrogel can be useful as a 3D printing material for electrical applications. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/30889733/Development_of_3D_printable_conductive_hydrogel_with_crystallized_PEDOT:PSS_for_neural_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(18)32659-6 DB - PRIME DP - Unbound Medicine ER -