Tags

Type your tag names separated by a space and hit enter

Nitric oxide synthase-mediated early nitric oxide burst alleviates water stress-induced oxidative damage in ammonium-supplied rice roots.
BMC Plant Biol. 2019 Mar 20; 19(1):108.BP

Abstract

BACKGROUND

Nutrition with ammonium (NH4+) can enhance the drought tolerance of rice seedlings in comparison to nutrition with nitrate (NO3-). However, there are still no detailed studies investigating the response of nitric oxide (NO) to the different nitrogen nutrition and water regimes. To study the intrinsic mechanism underpinning this relationship, the time-dependent production of NO and its protective role in the antioxidant defense system of NH4+- or NO3--supplied rice seedlings were studied under water stress.

RESULTS

An early NO burst was induced by 3 h of water stress in the roots of seedlings subjected to NH4+ treatment, but this phenomenon was not observed under NO3- treatment. Root oxidative damage induced by water stress was significantly higher for treatment with NO3- than with NH4+ due to reactive oxygen species (ROS) accumulation in the former. Inducing NO production by applying the NO donor 3 h after NO3- treatment alleviated the oxidative damage, while inhibiting the early NO burst by applying the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) increased root oxidative damage in NH4+ treatment. Application of the nitric oxide synthase (NOS) inhibitor N(G)-nitro-L-arginine methyl ester(L-NAME) completely suppressed NO synthesis in roots 3 h after NH4+ treatment and aggravated water stress-induced oxidative damage. Therefore, the aggravation of oxidative damage by L-NAME might have resulted from changes in the NOS-mediated early NO burst. Water stress also increased the activity of root antioxidant enzymes (catalase, superoxide dismutase, and ascorbate peroxidase). These were further induced by the NO donor but repressed by the NO scavenger and NOS inhibitor in NH4+-treated roots.

CONCLUSION

These findings demonstrate that the NOS-mediated early NO burst plays an important role in alleviating oxidative damage induced by water stress by enhancing the antioxidant defenses in roots supplemented with NH4+.

Links

PMC Free PDF

Authors+Show Affiliations

Cao X
State Key Laboratory of Rice Biology, China National Rice Research Institute, No. 359 Tiyuchang Road, Hangzhou, Zhejiang, 310006, People's Republic of China.
Zhu C
State Key Laboratory of Rice Biology, China National Rice Research Institute, No. 359 Tiyuchang Road, Hangzhou, Zhejiang, 310006, People's Republic of China.
Zhong C
State Key Laboratory of Rice Biology, China National Rice Research Institute, No. 359 Tiyuchang Road, Hangzhou, Zhejiang, 310006, People's Republic of China.
Zhang J
State Key Laboratory of Rice Biology, China National Rice Research Institute, No. 359 Tiyuchang Road, Hangzhou, Zhejiang, 310006, People's Republic of China.
Wu L
Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Jin Q
State Key Laboratory of Rice Biology, China National Rice Research Institute, No. 359 Tiyuchang Road, Hangzhou, Zhejiang, 310006, People's Republic of China. 11014041@zju.edu.cn.
Ma Q
Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China. 592214410@qq.com.

MeSH

Ammonium CompoundsAntioxidantsArginineCitrullineDehydrationNG-Nitroarginine Methyl EsterNitratesNitric OxideNitric Oxide DonorsNitric Oxide SynthaseNitroprussideOryzaOxidation-ReductionPlant ProteinsPlant Roots

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30894123

Citation

Cao, Xiaochuang, et al. "Nitric Oxide Synthase-mediated Early Nitric Oxide Burst Alleviates Water Stress-induced Oxidative Damage in Ammonium-supplied Rice Roots." BMC Plant Biology, vol. 19, no. 1, 2019, p. 108.
Cao X, Zhu C, Zhong C, et al. Nitric oxide synthase-mediated early nitric oxide burst alleviates water stress-induced oxidative damage in ammonium-supplied rice roots. BMC Plant Biol. 2019;19(1):108.
Cao, X., Zhu, C., Zhong, C., Zhang, J., Wu, L., Jin, Q., & Ma, Q. (2019). Nitric oxide synthase-mediated early nitric oxide burst alleviates water stress-induced oxidative damage in ammonium-supplied rice roots. BMC Plant Biology, 19(1), 108. https://doi.org/10.1186/s12870-019-1721-2
Cao X, et al. Nitric Oxide Synthase-mediated Early Nitric Oxide Burst Alleviates Water Stress-induced Oxidative Damage in Ammonium-supplied Rice Roots. BMC Plant Biol. 2019 Mar 20;19(1):108. PubMed PMID: 30894123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide synthase-mediated early nitric oxide burst alleviates water stress-induced oxidative damage in ammonium-supplied rice roots. AU - Cao,Xiaochuang, AU - Zhu,Chunquan, AU - Zhong,Chu, AU - Zhang,Junhua, AU - Wu,Lianghuan, AU - Jin,Qianyu, AU - Ma,Qingxu, Y1 - 2019/03/20/ PY - 2018/10/30/received PY - 2019/03/14/accepted PY - 2019/3/22/entrez PY - 2019/3/22/pubmed PY - 2019/5/7/medline KW - Ammonium KW - Antioxidant enzymes KW - Nitric oxide KW - Nitric oxide synthase KW - Oxidative damage KW - Water stress SP - 108 EP - 108 JF - BMC plant biology JO - BMC Plant Biol VL - 19 IS - 1 N2 - BACKGROUND: Nutrition with ammonium (NH4+) can enhance the drought tolerance of rice seedlings in comparison to nutrition with nitrate (NO3-). However, there are still no detailed studies investigating the response of nitric oxide (NO) to the different nitrogen nutrition and water regimes. To study the intrinsic mechanism underpinning this relationship, the time-dependent production of NO and its protective role in the antioxidant defense system of NH4+- or NO3--supplied rice seedlings were studied under water stress. RESULTS: An early NO burst was induced by 3 h of water stress in the roots of seedlings subjected to NH4+ treatment, but this phenomenon was not observed under NO3- treatment. Root oxidative damage induced by water stress was significantly higher for treatment with NO3- than with NH4+ due to reactive oxygen species (ROS) accumulation in the former. Inducing NO production by applying the NO donor 3 h after NO3- treatment alleviated the oxidative damage, while inhibiting the early NO burst by applying the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) increased root oxidative damage in NH4+ treatment. Application of the nitric oxide synthase (NOS) inhibitor N(G)-nitro-L-arginine methyl ester(L-NAME) completely suppressed NO synthesis in roots 3 h after NH4+ treatment and aggravated water stress-induced oxidative damage. Therefore, the aggravation of oxidative damage by L-NAME might have resulted from changes in the NOS-mediated early NO burst. Water stress also increased the activity of root antioxidant enzymes (catalase, superoxide dismutase, and ascorbate peroxidase). These were further induced by the NO donor but repressed by the NO scavenger and NOS inhibitor in NH4+-treated roots. CONCLUSION: These findings demonstrate that the NOS-mediated early NO burst plays an important role in alleviating oxidative damage induced by water stress by enhancing the antioxidant defenses in roots supplemented with NH4+. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/30894123/Nitric_oxide_synthase_mediated_early_nitric_oxide_burst_alleviates_water_stress_induced_oxidative_damage_in_ammonium_supplied_rice_roots_ DB - PRIME DP - Unbound Medicine ER -