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Differential gene expression in response to eCry3.1Ab ingestion in an unselected and eCry3.1Ab-selected western corn rootworm (Diabrotica virgifera virgifera LeConte) population.
Sci Rep. 2019 03 20; 9(1):4896.SR

Abstract

Diabrotica virgifera virgifera LeConte, the western corn rootworm (WCR) is one of the most destructive pests in the U.S. Corn Belt. Transgenic maize lines expressing various Cry toxins from Bacillus thuringiensis have been adopted as a management strategy. However, resistance to many Bt toxins has occurred. To investigate the mechanisms of Bt resistance we carried out RNA-seq using Illumina sequencing technology on resistant, eCry3.1Ab-selected and susceptible, unselected, whole WCR neonates which fed on seedling maize with and without eCry3.1Ab for 12 and 24 hours. In a parallel experiment RNA-seq experiments were conducted when only the midgut of neonate WCR was evaluated from the same treatments. After de novo transcriptome assembly we identified differentially expressed genes (DEGs). Results from the assemblies and annotation indicate that WCR neonates from the eCry3.1Ab-selected resistant colony expressed a small number of up and down-regulated genes following Bt intoxication. In contrast, unselected susceptible WCR neonates expressed a large number of up and down-regulated transcripts in response to intoxication. Annotation and pathway analysis of DEGs between susceptible and resistant whole WCR and their midgut tissue revealed genes associated with cell membrane, immune response, detoxification, and potential Bt receptors which are likely related to eCry3.1Ab resistance. This research provides a framework to study the toxicology of Bt toxins and mechanism of resistance in WCR, an economically important coleopteran pest species.

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Authors+Show Affiliations

Zhao Z
Division of Plant Sciences, University of Missouri, Columbia, MO, USA.
Meihls LN
Division of Plant Sciences, University of Missouri, Columbia, MO, USA. USDA-ARS, Columbia, MO, Columbia, MO, USA.
Hibbard BE
Division of Plant Sciences, University of Missouri, Columbia, MO, USA. USDA-ARS, Columbia, MO, Columbia, MO, USA.
Ji T
Department of Statistics, University of Missouri, Columbia, MO, USA.
Elsik CG
Division of Plant Sciences, University of Missouri, Columbia, MO, USA. Division of Animal Sciences, University of Missouri, Columbia, MO, USA. MU Informatics Institute, University of Missouri, Columbia, MO, USA.
Shelby KS
Division of Plant Sciences, University of Missouri, Columbia, MO, USA. Kent.Shelby@ars.usda.gov. USDA-ARS, Columbia, MO, Columbia, MO, USA. Kent.Shelby@ars.usda.gov.

MeSH

AnimalsBacillus thuringiensisBacillus thuringiensis ToxinsBacterial ProteinsColeopteraEndotoxinsHemolysin ProteinsInsecticide ResistanceLarvaPest Control, BiologicalPlants, Genetically ModifiedZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30894586

Citation

Zhao, Zixiao, et al. "Differential Gene Expression in Response to eCry3.1Ab Ingestion in an Unselected and eCry3.1Ab-selected Western Corn Rootworm (Diabrotica Virgifera Virgifera LeConte) Population." Scientific Reports, vol. 9, no. 1, 2019, p. 4896.
Zhao Z, Meihls LN, Hibbard BE, et al. Differential gene expression in response to eCry3.1Ab ingestion in an unselected and eCry3.1Ab-selected western corn rootworm (Diabrotica virgifera virgifera LeConte) population. Sci Rep. 2019;9(1):4896.
Zhao, Z., Meihls, L. N., Hibbard, B. E., Ji, T., Elsik, C. G., & Shelby, K. S. (2019). Differential gene expression in response to eCry3.1Ab ingestion in an unselected and eCry3.1Ab-selected western corn rootworm (Diabrotica virgifera virgifera LeConte) population. Scientific Reports, 9(1), 4896. https://doi.org/10.1038/s41598-019-41067-7
Zhao Z, et al. Differential Gene Expression in Response to eCry3.1Ab Ingestion in an Unselected and eCry3.1Ab-selected Western Corn Rootworm (Diabrotica Virgifera Virgifera LeConte) Population. Sci Rep. 2019 03 20;9(1):4896. PubMed PMID: 30894586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential gene expression in response to eCry3.1Ab ingestion in an unselected and eCry3.1Ab-selected western corn rootworm (Diabrotica virgifera virgifera LeConte) population. AU - Zhao,Zixiao, AU - Meihls,Lisa N, AU - Hibbard,Bruce E, AU - Ji,Tieming, AU - Elsik,Christine G, AU - Shelby,Kent S, Y1 - 2019/03/20/ PY - 2018/07/13/received PY - 2019/02/26/accepted PY - 2019/3/22/entrez PY - 2019/3/22/pubmed PY - 2020/10/21/medline SP - 4896 EP - 4896 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - Diabrotica virgifera virgifera LeConte, the western corn rootworm (WCR) is one of the most destructive pests in the U.S. Corn Belt. Transgenic maize lines expressing various Cry toxins from Bacillus thuringiensis have been adopted as a management strategy. However, resistance to many Bt toxins has occurred. To investigate the mechanisms of Bt resistance we carried out RNA-seq using Illumina sequencing technology on resistant, eCry3.1Ab-selected and susceptible, unselected, whole WCR neonates which fed on seedling maize with and without eCry3.1Ab for 12 and 24 hours. In a parallel experiment RNA-seq experiments were conducted when only the midgut of neonate WCR was evaluated from the same treatments. After de novo transcriptome assembly we identified differentially expressed genes (DEGs). Results from the assemblies and annotation indicate that WCR neonates from the eCry3.1Ab-selected resistant colony expressed a small number of up and down-regulated genes following Bt intoxication. In contrast, unselected susceptible WCR neonates expressed a large number of up and down-regulated transcripts in response to intoxication. Annotation and pathway analysis of DEGs between susceptible and resistant whole WCR and their midgut tissue revealed genes associated with cell membrane, immune response, detoxification, and potential Bt receptors which are likely related to eCry3.1Ab resistance. This research provides a framework to study the toxicology of Bt toxins and mechanism of resistance in WCR, an economically important coleopteran pest species. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/30894586/Differential_gene_expression_in_response_to_eCry3_1Ab_ingestion_in_an_unselected_and_eCry3_1Ab_selected_western_corn_rootworm__Diabrotica_virgifera_virgifera_LeConte__population_ DB - PRIME DP - Unbound Medicine ER -
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