Tags

Type your tag names separated by a space and hit enter

Qualitative and Quantitative Analysis of Cathinones in Human Urine by SPE-GC-MS.
Fa Yi Xue Za Zhi. 2018 Jun; 34(6):606-610.FY

Abstract

OBJECTIVES

To propose an alternative solid phase extraction-gas chromatography-mass spectrometry (SPE-GC-MS) method for the sensitive determination of cathinones in human urine samples, plus methodological verification.

METHODS

Human urine samples were concentrated by solid phase extraction (SPE) sorbent and converted into the corresponding heptafluorobutyric acid anhydride derivatives. Methcathinone, 4-methyl methcathinone and 3,4-methylenedioxy-N-methylcathinone were detected by gas chromatography-mass spectrometry (GC-MS). With quantitative analysis by internal standard method, methodological verification was carried out from the aspects of specificity, precision and reco-very rate.

RESULTS

Methcathinone, 4-methyl methcathinone and 3,4-methylenedioxy-N-methylcathinone showed good linearly in the range of 25-200 ng/mL of urine (r>0.99), with the limits of detection 2.0 ng/mL, limit of quantitation 25.0 ng/mL, both intra- and inter-day coefficients of variation lower than 6.0%, and recovery rates of 98.4%-105.7%.

CONCLUSIONS

he proposed SPE-GC-MS procedure has good accuracy and specificity, can meet the need of qualitative and quantitative analysis of cathinones in the urine of drug abusers, and therefore provide technical support for the detection of cathinone abuse.

Authors+Show Affiliations

Guangzhou Accurate and Correct Test Company, Guangzhou 510663, China. Guangdong Accurate and Correct Institution of Forensic Sciences, Guangzhou 510663, China.Guangzhou Accurate and Correct Test Company, Guangzhou 510663, China. Guangdong Accurate and Correct Institution of Forensic Sciences, Guangzhou 510663, China. Guangzhou Wondfo Biotech Co. Ltd., Guangzhou 510663, China.Guangzhou Accurate and Correct Test Company, Guangzhou 510663, China. Guangdong Accurate and Correct Institution of Forensic Sciences, Guangzhou 510663, China.Guangzhou Accurate and Correct Test Company, Guangzhou 510663, China. Guangdong Accurate and Correct Institution of Forensic Sciences, Guangzhou 510663, China.Guangzhou Accurate and Correct Test Company, Guangzhou 510663, China. Guangdong Accurate and Correct Institution of Forensic Sciences, Guangzhou 510663, China.

Pub Type(s)

Journal Article

Language

eng chi

PubMed ID

30896097

Citation

Wang, P, et al. "Qualitative and Quantitative Analysis of Cathinones in Human Urine By SPE-GC-MS." Fa Yi Xue Za Zhi, vol. 34, no. 6, 2018, pp. 606-610.
Wang P, Liu XY, Liu Y, et al. Qualitative and Quantitative Analysis of Cathinones in Human Urine by SPE-GC-MS. Fa Yi Xue Za Zhi. 2018;34(6):606-610.
Wang, P., Liu, X. Y., Liu, Y., Luo, Y. F., & Wang, Z. (2018). Qualitative and Quantitative Analysis of Cathinones in Human Urine by SPE-GC-MS. Fa Yi Xue Za Zhi, 34(6), 606-610. https://doi.org/10.12116/j.issn.1004-5619.2018.06.006
Wang P, et al. Qualitative and Quantitative Analysis of Cathinones in Human Urine By SPE-GC-MS. Fa Yi Xue Za Zhi. 2018;34(6):606-610. PubMed PMID: 30896097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Qualitative and Quantitative Analysis of Cathinones in Human Urine by SPE-GC-MS. AU - Wang,P, AU - Liu,X Y, AU - Liu,Y, AU - Luo,Y F, AU - Wang,Z, Y1 - 2018/12/25/ PY - 2017/02/14/received PY - 2019/3/22/entrez PY - 2019/3/22/pubmed PY - 2019/6/14/medline KW - forensic toxicology; solid phase extraction; gas chromatography-mass spectrometry; methcathinone; 4-methyl methcathinone; 3,4-methylenedioxy-N-methylcathinone; urine SP - 606 EP - 610 JF - Fa yi xue za zhi JO - Fa Yi Xue Za Zhi VL - 34 IS - 6 N2 - OBJECTIVES: To propose an alternative solid phase extraction-gas chromatography-mass spectrometry (SPE-GC-MS) method for the sensitive determination of cathinones in human urine samples, plus methodological verification. METHODS: Human urine samples were concentrated by solid phase extraction (SPE) sorbent and converted into the corresponding heptafluorobutyric acid anhydride derivatives. Methcathinone, 4-methyl methcathinone and 3,4-methylenedioxy-N-methylcathinone were detected by gas chromatography-mass spectrometry (GC-MS). With quantitative analysis by internal standard method, methodological verification was carried out from the aspects of specificity, precision and reco-very rate. RESULTS: Methcathinone, 4-methyl methcathinone and 3,4-methylenedioxy-N-methylcathinone showed good linearly in the range of 25-200 ng/mL of urine (r>0.99), with the limits of detection 2.0 ng/mL, limit of quantitation 25.0 ng/mL, both intra- and inter-day coefficients of variation lower than 6.0%, and recovery rates of 98.4%-105.7%. CONCLUSIONS: he proposed SPE-GC-MS procedure has good accuracy and specificity, can meet the need of qualitative and quantitative analysis of cathinones in the urine of drug abusers, and therefore provide technical support for the detection of cathinone abuse. SN - 1004-5619 UR - https://www.unboundmedicine.com/medline/citation/30896097/Qualitative_and_Quantitative_Analysis_of_Cathinones_in_Human_Urine_by_SPE_GC_MS_ L2 - https://doi.org/10.12116/j.issn.1004-5619.2018.06.006 DB - PRIME DP - Unbound Medicine ER -