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Expression analysis of transfer RNA‑derived fragments in the blood of patients with moyamoya disease: A preliminary study.
Mol Med Rep 2019; 19(5):3564-3574MM

Abstract

Moyamoya disease (MMD) is a rare chronic cerebrovascular disease mainly found in individuals of East Asian ethnicity, and its pathogenesis is largely unknown. Transfer RNA‑derived fragments (tRFs) are novel biological entities involved in many biological processes; however, whether tRFs contribute towards MMD pathogenesis remains unexplored. In the present study, deep sequencing technology was used to identify alterations in tRF expression profiles between patients with MMD and healthy controls. The sequencing findings were validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Subsequently, the putative target genes of tRFs were predicted using miRNA target prediction algorithms. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to further evaluate potential functions of tRFs. The sequencing results demonstrated that 38 tRFs were differentially expressed between patients and controls, of which 22 were upregulated and 16 were downregulated. RT‑qPCR analysis confirmed the validity of the sequencing results. GO and KEGG pathway enrichment analyses indicated that 15 pathways were associated with the selected tRFs. These pathways were mainly involved in angiogenesis and metabolism, both of which are physiopathological fundamentals of MMD. The results provided a novel insight into the mechanisms underlying MMD pathogenesis, and demonstrated that tRFs may serve as potential therapeutic targets for the future treatment of MMD.

Authors+Show Affiliations

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30896793

Citation

Wang, Chengjun, et al. "Expression Analysis of Transfer RNA‑derived Fragments in the Blood of Patients With Moyamoya Disease: a Preliminary Study." Molecular Medicine Reports, vol. 19, no. 5, 2019, pp. 3564-3574.
Wang C, Zhao M, Wang J, et al. Expression analysis of transfer RNA‑derived fragments in the blood of patients with moyamoya disease: A preliminary study. Mol Med Rep. 2019;19(5):3564-3574.
Wang, C., Zhao, M., Wang, J., Zhang, D., Wang, S., & Zhao, J. (2019). Expression analysis of transfer RNA‑derived fragments in the blood of patients with moyamoya disease: A preliminary study. Molecular Medicine Reports, 19(5), pp. 3564-3574. doi:10.3892/mmr.2019.10024.
Wang C, et al. Expression Analysis of Transfer RNA‑derived Fragments in the Blood of Patients With Moyamoya Disease: a Preliminary Study. Mol Med Rep. 2019;19(5):3564-3574. PubMed PMID: 30896793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression analysis of transfer RNA‑derived fragments in the blood of patients with moyamoya disease: A preliminary study. AU - Wang,Chengjun, AU - Zhao,Meng, AU - Wang,Jia, AU - Zhang,Dong, AU - Wang,Shuo, AU - Zhao,Jizong, Y1 - 2019/03/14/ PY - 2018/05/08/received PY - 2019/01/14/accepted PY - 2019/3/22/pubmed PY - 2019/8/2/medline PY - 2019/3/22/entrez SP - 3564 EP - 3574 JF - Molecular medicine reports JO - Mol Med Rep VL - 19 IS - 5 N2 - Moyamoya disease (MMD) is a rare chronic cerebrovascular disease mainly found in individuals of East Asian ethnicity, and its pathogenesis is largely unknown. Transfer RNA‑derived fragments (tRFs) are novel biological entities involved in many biological processes; however, whether tRFs contribute towards MMD pathogenesis remains unexplored. In the present study, deep sequencing technology was used to identify alterations in tRF expression profiles between patients with MMD and healthy controls. The sequencing findings were validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Subsequently, the putative target genes of tRFs were predicted using miRNA target prediction algorithms. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to further evaluate potential functions of tRFs. The sequencing results demonstrated that 38 tRFs were differentially expressed between patients and controls, of which 22 were upregulated and 16 were downregulated. RT‑qPCR analysis confirmed the validity of the sequencing results. GO and KEGG pathway enrichment analyses indicated that 15 pathways were associated with the selected tRFs. These pathways were mainly involved in angiogenesis and metabolism, both of which are physiopathological fundamentals of MMD. The results provided a novel insight into the mechanisms underlying MMD pathogenesis, and demonstrated that tRFs may serve as potential therapeutic targets for the future treatment of MMD. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/30896793/Expression_analysis_of_transfer_RNA‑derived_fragments_in_the_blood_of_patients_with_moyamoya_disease:_A_preliminary_study_ L2 - http://www.spandidos-publications.com/mmr/19/5/3564 DB - PRIME DP - Unbound Medicine ER -