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Whole blood human transcriptome and virome analysis of ME/CFS patients experiencing post-exertional malaise following cardiopulmonary exercise testing.
PLoS One. 2019; 14(3):e0212193.Plos

Abstract

Myalgic encephalomyelitis / chronic fatigue syndrome (ME/CFS) is a syndrome of unknown etiology characterized by profound fatigue exacerbated by physical activity, also known as post-exertional malaise (PEM). Previously, we did not detect evidence of immune dysregulation or virus reactivation outside of PEM periods. Here we sought to determine whether cardiopulmonary exercise stress testing of ME/CFS patients could trigger such changes. ME/CFS patients (n = 14) and matched sedentary controls (n = 11) were subjected to cardiopulmonary exercise on 2 consecutive days and followed up to 7 days post-exercise, and longitudinal whole blood samples analyzed by RNA-seq. Although ME/CFS patients showed significant worsening of symptoms following exercise versus controls, with 8 of 14 ME/CFS patients showing reduced oxygen consumption ([Formula: see text]) on day 2, transcriptome analysis yielded only 6 differentially expressed gene (DEG) candidates when comparing ME/CFS patients to controls across all time points. None of the DEGs were related to immune signaling, and no DEGs were found in ME/CFS patients before and after exercise. Virome composition (P = 0.746 by chi-square test) and number of viral reads (P = 0.098 by paired t-test) were not significantly associated with PEM. These observations do not support transcriptionally-mediated immune cell dysregulation or viral reactivation in ME/CFS patients during symptomatic PEM episodes.

Authors+Show Affiliations

Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America.Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America.Communicable Disease Prevention and Control Services, Vancouver, Canada. School of Population and Public Health, University of British Columbia, Vancouver, Canada.School of Population and Public Health, University of British Columbia, Vancouver, Canada.Workwell Foundation, Ripon, California, United States of America.Workwell Foundation, Ripon, California, United States of America.Workwell Foundation, Ripon, California, United States of America.Workwell Foundation, Ripon, California, United States of America.Department of Pediatrics, Division of Cardiology, University of British Columbia, Vancouver, Canada.School of Population and Public Health, University of British Columbia, Vancouver, Canada.British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada. Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.Communicable Disease Prevention and Control Services, Vancouver, Canada.Centre for Health Evaluation Outcome Sciences, Vancouver, Canada.British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.Department of Pathology, Sidra Medical and Research Center, Doha, Qatar.Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, Canada.Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, Canada.Division of Cardiology, British Columbia's Children's Hospital, Vancouver, Canada.Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada. Adult Metabolic Disease Clinic, Vancouver General Hospital, Vancouver, Canada.Communicable Disease Prevention and Control Services, Vancouver, Canada. School of Population and Public Health, University of British Columbia, Vancouver, Canada.Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, United States of America. Department of Medicine, Division of Infectious Diseases, University of California San Francisco, San Francisco, California, United States of America.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30897114

Citation

Bouquet, Jerome, et al. "Whole Blood Human Transcriptome and Virome Analysis of ME/CFS Patients Experiencing Post-exertional Malaise Following Cardiopulmonary Exercise Testing." PloS One, vol. 14, no. 3, 2019, pp. e0212193.
Bouquet J, Li T, Gardy JL, et al. Whole blood human transcriptome and virome analysis of ME/CFS patients experiencing post-exertional malaise following cardiopulmonary exercise testing. PLoS One. 2019;14(3):e0212193.
Bouquet, J., Li, T., Gardy, J. L., Kang, X., Stevens, S., Stevens, J., VanNess, M., Snell, C., Potts, J., Miller, R. R., Morshed, M., McCabe, M., Parker, S., Uyaguari, M., Tang, P., Steiner, T., Chan, W. S., De Souza, A. M., Mattman, A., ... Chiu, C. Y. (2019). Whole blood human transcriptome and virome analysis of ME/CFS patients experiencing post-exertional malaise following cardiopulmonary exercise testing. PloS One, 14(3), e0212193. https://doi.org/10.1371/journal.pone.0212193
Bouquet J, et al. Whole Blood Human Transcriptome and Virome Analysis of ME/CFS Patients Experiencing Post-exertional Malaise Following Cardiopulmonary Exercise Testing. PLoS One. 2019;14(3):e0212193. PubMed PMID: 30897114.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Whole blood human transcriptome and virome analysis of ME/CFS patients experiencing post-exertional malaise following cardiopulmonary exercise testing. AU - Bouquet,Jerome, AU - Li,Tony, AU - Gardy,Jennifer L, AU - Kang,Xiaoying, AU - Stevens,Staci, AU - Stevens,Jared, AU - VanNess,Mark, AU - Snell,Christopher, AU - Potts,James, AU - Miller,Ruth R, AU - Morshed,Muhammad, AU - McCabe,Mark, AU - Parker,Shoshana, AU - Uyaguari,Miguel, AU - Tang,Patrick, AU - Steiner,Theodore, AU - Chan,Wee-Shian, AU - De Souza,Astrid-Marie, AU - Mattman,Andre, AU - Patrick,David M, AU - Chiu,Charles Y, Y1 - 2019/03/21/ PY - 2018/06/21/received PY - 2019/01/24/accepted PY - 2019/3/22/entrez PY - 2019/3/22/pubmed PY - 2020/1/7/medline SP - e0212193 EP - e0212193 JF - PloS one JO - PLoS One VL - 14 IS - 3 N2 - Myalgic encephalomyelitis / chronic fatigue syndrome (ME/CFS) is a syndrome of unknown etiology characterized by profound fatigue exacerbated by physical activity, also known as post-exertional malaise (PEM). Previously, we did not detect evidence of immune dysregulation or virus reactivation outside of PEM periods. Here we sought to determine whether cardiopulmonary exercise stress testing of ME/CFS patients could trigger such changes. ME/CFS patients (n = 14) and matched sedentary controls (n = 11) were subjected to cardiopulmonary exercise on 2 consecutive days and followed up to 7 days post-exercise, and longitudinal whole blood samples analyzed by RNA-seq. Although ME/CFS patients showed significant worsening of symptoms following exercise versus controls, with 8 of 14 ME/CFS patients showing reduced oxygen consumption ([Formula: see text]) on day 2, transcriptome analysis yielded only 6 differentially expressed gene (DEG) candidates when comparing ME/CFS patients to controls across all time points. None of the DEGs were related to immune signaling, and no DEGs were found in ME/CFS patients before and after exercise. Virome composition (P = 0.746 by chi-square test) and number of viral reads (P = 0.098 by paired t-test) were not significantly associated with PEM. These observations do not support transcriptionally-mediated immune cell dysregulation or viral reactivation in ME/CFS patients during symptomatic PEM episodes. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/30897114/Whole_blood_human_transcriptome_and_virome_analysis_of_ME/CFS_patients_experiencing_post_exertional_malaise_following_cardiopulmonary_exercise_testing_ L2 - https://dx.plos.org/10.1371/journal.pone.0212193 DB - PRIME DP - Unbound Medicine ER -