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Simultaneous determination of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study of Huangqi Guizhi Wuwu Decoction.
J Pharm Biomed Anal. 2019 Jun 05; 170:1-7.JP

Abstract

Huangqi Guizhi Wuwu Decoction (HGWWD), consisting of Radix Astragali, Cinnamomi Ramulus, Paeoniae Radix Alba, Zingiberis Rhizoma Recens and Jujubae Fructus, is a widely used Traditional Chinese Medicine (TCM) formula for the treatment of human blood impediment in China for nearly 2000 years. In order to make good and rational use of this formula in the future, a rapid, sensitive and robust ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for simultaneous determination of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin, the main active constituents of HGWWD, in rat plasma using geniposide as internal standard (IS). The plasma samples were extracted by protein precipitation with acetonitrile and separated on a Shim-pack XR-ODS C18 column (75 mm × 3.0 mm, 2.2 μm) using gradient elution with a mobile phase consisting of water (containing 0.1% formic acid) and acetonitrile at a flow rate of 0.4 mL/min. Mass spectrometric detection was performed on 3200 QTRAP mass spectrometry equipped with electrospray ionization source in negative ionization mode. Quantification was performed using multiple reaction monitoring (MRM) by monitoring the fragmentation of m/z 491.1→282.9 for calycosin-7-O-β-D-glucoside, m/z147.0→103.1 for cinnamic acid, m/z 525.0→120.9 for paeoniflorin, m/z 525.2→121.0 for albiflorin and m/z 433.1→225.1 for IS, respectively. The method was well validated in terms of linearity, precision, accuracy, recovery, matrix effect and stability. All calibration curves had good linearity (r>0.9977) over the concentration range from 0.1-50 ng/mL for calycosin-7-O-β-D-glycoside, 50-25000 ng/mL for cinnamic acid, 5-2500 ng/mL for paeoniflorin and albiflorin. The intra-day and inter-day precisions (relative standard deviation) were within 11.8%, the accuracy (relative error) ranged from -9.4% to 9.1%, and the lower limit of quantification (LLOQ) were 0.1, 50, 5, 5 ng/mL for calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin, respectively. Extraction recovery, matrix effect and stability were satisfactory in rat plasma. The validated method was successfully applied to a pharmacokinetic study of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin after oral administration of HGWWD to rats.

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Authors+Show Affiliations

School of Pharmacy, Jilin Medical University, 5 Jilin Street, Fengman District, Jilin, 132013, China.

School of Pharmacy, Jilin Medical University, 5 Jilin Street, Fengman District, Jilin, 132013, China.

School of Pharmacy, Jilin Medical University, 5 Jilin Street, Fengman District, Jilin, 132013, China.

School of Pharmacy, Jilin Medical University, 5 Jilin Street, Fengman District, Jilin, 132013, China.

School of Pharmacy, Jilin Medical University, 5 Jilin Street, Fengman District, Jilin, 132013, China.

School of Pharmacy, Jilin Medical University, 5 Jilin Street, Fengman District, Jilin, 132013, China. Electronic address: fengbo2@sina.com.

School of Pharmacy, Jilin Medical University, 5 Jilin Street, Fengman District, Jilin, 132013, China. Electronic address: zhy19820903@126.com.

MeSH

AnimalsBridged-Ring CompoundsChinaChromatography, High Pressure LiquidCinnamatesDrugs, Chinese HerbalGlucosidesIsoflavonesMaleMedicine, Chinese TraditionalMonoterpenesPaeoniaPlasmaRatsRats, Sprague-DawleyTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30897430

Citation

Guan, Jiao, et al. "Simultaneous Determination of calycosin-7-O-β-D-glucoside, Cinnamic Acid, Paeoniflorin and Albiflorin in Rat Plasma By UHPLC-MS/MS and Its Application to a Pharmacokinetic Study of Huangqi Guizhi Wuwu Decoction." Journal of Pharmaceutical and Biomedical Analysis, vol. 170, 2019, pp. 1-7.

Guan J, Wang L, Jin J, et al. Simultaneous determination of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study of Huangqi Guizhi Wuwu Decoction. J Pharm Biomed Anal. 2019;170:1-7.

Guan, J., Wang, L., Jin, J., Chang, S., Xiao, X., Feng, B., & Zhu, H. (2019). Simultaneous determination of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study of Huangqi Guizhi Wuwu Decoction. Journal of Pharmaceutical and Biomedical Analysis, 170, 1-7. https://doi.org/10.1016/j.jpba.2019.03.022

Guan J, et al. Simultaneous Determination of calycosin-7-O-β-D-glucoside, Cinnamic Acid, Paeoniflorin and Albiflorin in Rat Plasma By UHPLC-MS/MS and Its Application to a Pharmacokinetic Study of Huangqi Guizhi Wuwu Decoction. J Pharm Biomed Anal. 2019 Jun 5;170:1-7. PubMed PMID: 30897430.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Simultaneous determination of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study of Huangqi Guizhi Wuwu Decoction. AU - Guan,Jiao, AU - Wang,Liming, AU - Jin,Jia, AU - Chang,Sheng, AU - Xiao,Xiao, AU - Feng,Bo, AU - Zhu,Heyun, Y1 - 2019/03/14/ PY - 2019/01/23/received PY - 2019/03/05/revised PY - 2019/03/12/accepted PY - 2019/3/22/pubmed PY - 2019/8/23/medline PY - 2019/3/22/entrez KW - Active constituents KW - Huangqi Guizhi Wuwu Decoction KW - Pharmacokinetics KW - Rat plasma KW - UHPLC-MS/MS SP - 1 EP - 7 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 170 N2 - Huangqi Guizhi Wuwu Decoction (HGWWD), consisting of Radix Astragali, Cinnamomi Ramulus, Paeoniae Radix Alba, Zingiberis Rhizoma Recens and Jujubae Fructus, is a widely used Traditional Chinese Medicine (TCM) formula for the treatment of human blood impediment in China for nearly 2000 years. In order to make good and rational use of this formula in the future, a rapid, sensitive and robust ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for simultaneous determination of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin, the main active constituents of HGWWD, in rat plasma using geniposide as internal standard (IS). The plasma samples were extracted by protein precipitation with acetonitrile and separated on a Shim-pack XR-ODS C18 column (75 mm × 3.0 mm, 2.2 μm) using gradient elution with a mobile phase consisting of water (containing 0.1% formic acid) and acetonitrile at a flow rate of 0.4 mL/min. Mass spectrometric detection was performed on 3200 QTRAP mass spectrometry equipped with electrospray ionization source in negative ionization mode. Quantification was performed using multiple reaction monitoring (MRM) by monitoring the fragmentation of m/z 491.1→282.9 for calycosin-7-O-β-D-glucoside, m/z147.0→103.1 for cinnamic acid, m/z 525.0→120.9 for paeoniflorin, m/z 525.2→121.0 for albiflorin and m/z 433.1→225.1 for IS, respectively. The method was well validated in terms of linearity, precision, accuracy, recovery, matrix effect and stability. All calibration curves had good linearity (r>0.9977) over the concentration range from 0.1-50 ng/mL for calycosin-7-O-β-D-glycoside, 50-25000 ng/mL for cinnamic acid, 5-2500 ng/mL for paeoniflorin and albiflorin. The intra-day and inter-day precisions (relative standard deviation) were within 11.8%, the accuracy (relative error) ranged from -9.4% to 9.1%, and the lower limit of quantification (LLOQ) were 0.1, 50, 5, 5 ng/mL for calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin, respectively. Extraction recovery, matrix effect and stability were satisfactory in rat plasma. The validated method was successfully applied to a pharmacokinetic study of calycosin-7-O-β-D-glucoside, cinnamic acid, paeoniflorin and albiflorin after oral administration of HGWWD to rats. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/30897430/Simultaneous_determination_of_calycosin_7_O_β_D_glucoside_cinnamic_acid_paeoniflorin_and_albiflorin_in_rat_plasma_by_UHPLC_MS/MS_and_its_application_to_a_pharmacokinetic_study_of_Huangqi_Guizhi_Wuwu_Decoction_ DB - PRIME DP - Unbound Medicine ER -