Tags

Type your tag names separated by a space and hit enter

Diallyl Trisulfide can induce fibroblast-like synovial apoptosis and has a therapeutic effect on collagen-induced arthritis in mice via blocking NF-κB and Wnt pathways.
Int Immunopharmacol. 2019 Jun; 71:132-138.II

Abstract

BACKGROUND

Diallyl Trisulfide (DATS) is an organosulfur compound extracted from garlic bulb, and exerts cardioprotective, anti-inflammatory, antioxidant, antimicrobial and anticancer effects. But its role in the pathogenesis of rheumatoid arthritis (RA) is unknown. Here we explored the influence of DATS on human fibroblast-like synoviocytes (FLS) isolated from RA patients and a mouse model of collagen-induced arthritis (CIA) and the underlying mechanism.

METHODS

RA-FLS were cultured and treated with different concentrations of DATS. The CCK8 assay was used to assess cell proliferation while cell apoptosis was detected by flow cytometry and western blot. The IL-8, IL-6 and IL-1β levels were determined using RT-qPCR and ELISA assay. The expression of proteins of the NF-κB and Wnt pathways were measured using western blot. Furthermore, the effect of DATS was also explored in vivo using the collagen-induced arthritis mouse model. The Th17/Treg pattern obtain from cells of spleen of collagen-induced arthritis mouse model was detected by flow cytometry.

RESULTS

Our results showed that DATS could decrease cell viability and introduce apoptosis in RA-FLS. Furthermore, DATS significantly attenuated the production of key inflammatory cytokines induced by RA-FLS cells following treatment with tumor necrosis α (TNF-α) at a concentration of 100 μM or higher. This was due to its inhibitory effect on the NF-κB and Wnt pathway signaling in RA-FLS. Additionally, DATS decreased the production of inflammatory cytokines and regulated the immune function by restoring the balance between Th17 and Treg in CIA mouse model.

CONCLUSIONS

In conclusion, DATS may serve as a potential curative agent for RA.

Authors+Show Affiliations

Department of Rheumatology and Immunology, Changhai Hospital, The Second Military Medical University/Naval Medical University, Shanghai, China; Department of Endocrinology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.Department of Rheumatology and Immunology, Changhai Hospital, The Second Military Medical University/Naval Medical University, Shanghai, China.Department of Plastic and Reconstructive Surgery, Changhai Hospital, Second Military Medical University/Naval Medical University, Shanghai, China.Department of Joint Bone Disease Surgery, Changhai Hospital, Second Military Medical University/Naval Medical University, Shanghai, China.Second Military Medical University/Naval Medical University, Shanghai, China.Department of Joint Bone Disease Surgery, Guanghua Hospital, Shanghai, China.Department of Rheumatology and Immunology, Changhai Hospital, The Second Military Medical University/Naval Medical University, Shanghai, China.Department of Rheumatology and Immunology, Changhai Hospital, The Second Military Medical University/Naval Medical University, Shanghai, China.Department of Rheumatology and Immunology, Changhai Hospital, The Second Military Medical University/Naval Medical University, Shanghai, China.Department of Rheumatology and Immunology, Changhai Hospital, The Second Military Medical University/Naval Medical University, Shanghai, China. Electronic address: dongbaozhao@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30897500

Citation

Liang, Jing Jing, et al. "Diallyl Trisulfide Can Induce Fibroblast-like Synovial Apoptosis and Has a Therapeutic Effect On Collagen-induced Arthritis in Mice Via Blocking NF-κB and Wnt Pathways." International Immunopharmacology, vol. 71, 2019, pp. 132-138.
Liang JJ, Li HR, Chen Y, et al. Diallyl Trisulfide can induce fibroblast-like synovial apoptosis and has a therapeutic effect on collagen-induced arthritis in mice via blocking NF-κB and Wnt pathways. Int Immunopharmacol. 2019;71:132-138.
Liang, J. J., Li, H. R., Chen, Y., Zhang, C., Chen, D. G., Liang, Z. C., Shi, Y. Q., Zhang, L. L., Xin, L., & Zhao, D. B. (2019). Diallyl Trisulfide can induce fibroblast-like synovial apoptosis and has a therapeutic effect on collagen-induced arthritis in mice via blocking NF-κB and Wnt pathways. International Immunopharmacology, 71, 132-138. https://doi.org/10.1016/j.intimp.2019.03.024
Liang JJ, et al. Diallyl Trisulfide Can Induce Fibroblast-like Synovial Apoptosis and Has a Therapeutic Effect On Collagen-induced Arthritis in Mice Via Blocking NF-κB and Wnt Pathways. Int Immunopharmacol. 2019;71:132-138. PubMed PMID: 30897500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diallyl Trisulfide can induce fibroblast-like synovial apoptosis and has a therapeutic effect on collagen-induced arthritis in mice via blocking NF-κB and Wnt pathways. AU - Liang,Jing Jing, AU - Li,Hao Ran, AU - Chen,Yong, AU - Zhang,Chen, AU - Chen,Da Gui, AU - Liang,Zhi Chao, AU - Shi,Ye Qing, AU - Zhang,Lan Ling, AU - Xin,Lei, AU - Zhao,Dong Bao, Y1 - 2019/03/18/ PY - 2018/10/26/received PY - 2019/03/08/revised PY - 2019/03/09/accepted PY - 2019/3/22/pubmed PY - 2019/3/22/medline PY - 2019/3/22/entrez KW - Apoptosis KW - Collagen-induced arthritis KW - Diallyl Trisulfide KW - Inflammatory KW - Rheumatoid arthritis KW - Synovial fibroblasts SP - 132 EP - 138 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 71 N2 - BACKGROUND: Diallyl Trisulfide (DATS) is an organosulfur compound extracted from garlic bulb, and exerts cardioprotective, anti-inflammatory, antioxidant, antimicrobial and anticancer effects. But its role in the pathogenesis of rheumatoid arthritis (RA) is unknown. Here we explored the influence of DATS on human fibroblast-like synoviocytes (FLS) isolated from RA patients and a mouse model of collagen-induced arthritis (CIA) and the underlying mechanism. METHODS: RA-FLS were cultured and treated with different concentrations of DATS. The CCK8 assay was used to assess cell proliferation while cell apoptosis was detected by flow cytometry and western blot. The IL-8, IL-6 and IL-1β levels were determined using RT-qPCR and ELISA assay. The expression of proteins of the NF-κB and Wnt pathways were measured using western blot. Furthermore, the effect of DATS was also explored in vivo using the collagen-induced arthritis mouse model. The Th17/Treg pattern obtain from cells of spleen of collagen-induced arthritis mouse model was detected by flow cytometry. RESULTS: Our results showed that DATS could decrease cell viability and introduce apoptosis in RA-FLS. Furthermore, DATS significantly attenuated the production of key inflammatory cytokines induced by RA-FLS cells following treatment with tumor necrosis α (TNF-α) at a concentration of 100 μM or higher. This was due to its inhibitory effect on the NF-κB and Wnt pathway signaling in RA-FLS. Additionally, DATS decreased the production of inflammatory cytokines and regulated the immune function by restoring the balance between Th17 and Treg in CIA mouse model. CONCLUSIONS: In conclusion, DATS may serve as a potential curative agent for RA. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/30897500/Diallyl_Trisulfide_can_induce_fibroblast_like_synovial_apoptosis_and_has_a_therapeutic_effect_on_collagen_induced_arthritis_in_mice_via_blocking_NF_κB_and_Wnt_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(18)30985-8 DB - PRIME DP - Unbound Medicine ER -