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Sensitizing of β-lactam resistance by tannic acid in methicillin-resistant S. aureus.
World J Microbiol Biotechnol. 2019 Mar 21; 35(4):57.WJ

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) infections treatment of which is hard and failed, due to being resistant to all types of β-lactams, have been emerged in hospitals and community. Long-term usage of antibiotics and over doses of antibiotics used in the treatment of infections cause bacteria to develop resistance to antibiotics. β-lactams combined with tannic acid can be a good alternative to sensitize the resistance of β-lactams used in the treatment of MRSA, due to their synergistic activities. In this study, after minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of sole tannic acid and β-lactam were investigated for each isolate, the synergistic activities of β-lactams combined with tannic acid against one MRSA ATCC 43300 and four MRSA clinical isolates were investigated with the concentrations starting at four fold of MICs of sole treatments of tannic acid and β-lactam by using checkerboard assay. To investigate sole and combination activities of tannic acid and β-lactams, MIC and MBCs were observed. Results of this study showed that the activities of β-lactams combined with tannic acid were synergistic and partially synergistic against MRSA isolates with FIC indexes ranged from 0.174 to 0.477 and 0.562 to 0.850, respectively. MIC of β-lactams were decreased 2-16 fold by sub-inhibitory concentrations of tannic acid without toxicity. Alternative treatment options of natural compounds such as tannic acid and β-lactams must be investigated further and developed to overcome the emergence of β-lactam resistance and treat MRSA infections by sensitizing the resistance of β-lactams.

Authors+Show Affiliations

Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Haliç University, 34445, Istanbul, Turkey. kirmusaoglu_sahra@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30900046

Citation

Kırmusaoğlu, Sahra. "Sensitizing of Β-lactam Resistance By Tannic Acid in Methicillin-resistant S. Aureus." World Journal of Microbiology & Biotechnology, vol. 35, no. 4, 2019, p. 57.
Kırmusaoğlu S. Sensitizing of β-lactam resistance by tannic acid in methicillin-resistant S. aureus. World J Microbiol Biotechnol. 2019;35(4):57.
Kırmusaoğlu, S. (2019). Sensitizing of β-lactam resistance by tannic acid in methicillin-resistant S. aureus. World Journal of Microbiology & Biotechnology, 35(4), 57. https://doi.org/10.1007/s11274-019-2637-6
Kırmusaoğlu S. Sensitizing of Β-lactam Resistance By Tannic Acid in Methicillin-resistant S. Aureus. World J Microbiol Biotechnol. 2019 Mar 21;35(4):57. PubMed PMID: 30900046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitizing of β-lactam resistance by tannic acid in methicillin-resistant S. aureus. A1 - Kırmusaoğlu,Sahra, Y1 - 2019/03/21/ PY - 2018/11/27/received PY - 2019/03/12/accepted PY - 2019/3/23/entrez PY - 2019/3/23/pubmed PY - 2019/4/23/medline KW - Antibacterial KW - Methicillin resistant Staphylococcus aureus (MRSA) KW - Sensitizing β-lactam resistance KW - Synergism KW - Tannic acid KW - β-Lactams SP - 57 EP - 57 JF - World journal of microbiology & biotechnology JO - World J. Microbiol. Biotechnol. VL - 35 IS - 4 N2 - Methicillin-resistant Staphylococcus aureus (MRSA) infections treatment of which is hard and failed, due to being resistant to all types of β-lactams, have been emerged in hospitals and community. Long-term usage of antibiotics and over doses of antibiotics used in the treatment of infections cause bacteria to develop resistance to antibiotics. β-lactams combined with tannic acid can be a good alternative to sensitize the resistance of β-lactams used in the treatment of MRSA, due to their synergistic activities. In this study, after minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of sole tannic acid and β-lactam were investigated for each isolate, the synergistic activities of β-lactams combined with tannic acid against one MRSA ATCC 43300 and four MRSA clinical isolates were investigated with the concentrations starting at four fold of MICs of sole treatments of tannic acid and β-lactam by using checkerboard assay. To investigate sole and combination activities of tannic acid and β-lactams, MIC and MBCs were observed. Results of this study showed that the activities of β-lactams combined with tannic acid were synergistic and partially synergistic against MRSA isolates with FIC indexes ranged from 0.174 to 0.477 and 0.562 to 0.850, respectively. MIC of β-lactams were decreased 2-16 fold by sub-inhibitory concentrations of tannic acid without toxicity. Alternative treatment options of natural compounds such as tannic acid and β-lactams must be investigated further and developed to overcome the emergence of β-lactam resistance and treat MRSA infections by sensitizing the resistance of β-lactams. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/30900046/Sensitizing_of_β_lactam_resistance_by_tannic_acid_in_methicillin_resistant_S__aureus_ L2 - https://dx.doi.org/10.1007/s11274-019-2637-6 DB - PRIME DP - Unbound Medicine ER -