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The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH.
Molecules. 2019 Mar 21; 24(6)M

Abstract

Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome. In this study, five different oligonucleotide probes were used with ND-FISH to examine 21 wheat cultivars and lines. These oligonucleotide probes distinguished 42 wheat chromosomes and also detected rye chromatin in the wheat genome. Moreover, the signal patterns of the oligonucleotide probes Oligo-pTa535-1 and Oligo-pSc119.2-1 showed high polymorphism in the wheat chromosomes. A total of 17.6% of the A group chromosomes, 25.9% of the B group chromosomes and 8.9% of the D group chromosomes showed obvious mutations when they were compared to the standard ND-FISH signal patterns, and most of them were Oligo-pSc119.2-1 mutants. The results suggested that these polymorphisms could be induced by the crossing of wheat cultivars. The results provided more information for the further application of oligonucleotide probes and ND-FISH.

Authors+Show Affiliations

College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. renth@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. renth@sicau.edu.cn.Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. hemaojieo@163.com. College of Life Science, Sichuan Agricultural University, Ya'an 625014, Sichuan, China. hemaojieo@163.com.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. szx080598@163.com. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. szx080598@163.com.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. tanfq@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. tanfq@sicau.edu.cn.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. lpglab@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. lpglab@sicau.edu.cn.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. zxtang@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. zxtang@sicau.edu.cn.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. fusl@sina.com. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. fusl@sina.com.Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. yanbenju@sicau.edu.cn. College of Life Science, Sichuan Agricultural University, Ya'an 625014, Sichuan, China. yanbenju@sicau.edu.cn.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. renzllab@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. renzllab@sicau.edu.cn.Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China. lizhi@sicau.edu.cn. College of Life Science, Sichuan Agricultural University, Ya'an 625014, Sichuan, China. lizhi@sicau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30901897

Citation

Ren, Tianheng, et al. "The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined By ND-FISH." Molecules (Basel, Switzerland), vol. 24, no. 6, 2019.
Ren T, He M, Sun Z, et al. The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH. Molecules. 2019;24(6).
Ren, T., He, M., Sun, Z., Tan, F., Luo, P., Tang, Z., Fu, S., Yan, B., Ren, Z., & Li, Z. (2019). The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH. Molecules (Basel, Switzerland), 24(6). https://doi.org/10.3390/molecules24061126
Ren T, et al. The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined By ND-FISH. Molecules. 2019 Mar 21;24(6) PubMed PMID: 30901897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH. AU - Ren,Tianheng, AU - He,Maojie, AU - Sun,Zixin, AU - Tan,Feiquan, AU - Luo,Peigao, AU - Tang,Zongxiang, AU - Fu,Shulan, AU - Yan,Benju, AU - Ren,Zhenglong, AU - Li,Zhi, Y1 - 2019/03/21/ PY - 2019/03/03/received PY - 2019/03/15/revised PY - 2019/03/19/accepted PY - 2019/3/24/entrez PY - 2019/3/25/pubmed PY - 2019/7/16/medline KW - ND-FISH KW - Oligo-pSc119.2-1 KW - Oligo-pTa535-1 KW - chromosome KW - mutant KW - wheat JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 6 N2 - Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome. In this study, five different oligonucleotide probes were used with ND-FISH to examine 21 wheat cultivars and lines. These oligonucleotide probes distinguished 42 wheat chromosomes and also detected rye chromatin in the wheat genome. Moreover, the signal patterns of the oligonucleotide probes Oligo-pTa535-1 and Oligo-pSc119.2-1 showed high polymorphism in the wheat chromosomes. A total of 17.6% of the A group chromosomes, 25.9% of the B group chromosomes and 8.9% of the D group chromosomes showed obvious mutations when they were compared to the standard ND-FISH signal patterns, and most of them were Oligo-pSc119.2-1 mutants. The results suggested that these polymorphisms could be induced by the crossing of wheat cultivars. The results provided more information for the further application of oligonucleotide probes and ND-FISH. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30901897/The_Polymorphisms_of_Oligonucleotide_Probes_in_Wheat_Cultivars_Determined_by_ND_FISH_ L2 - https://www.mdpi.com/resolver?pii=molecules24061126 DB - PRIME DP - Unbound Medicine ER -