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Critical parameters maintaining authentic CRAC channel hallmarks.
Eur Biophys J. 2019 Jul; 48(5):425-445.EB

Abstract

Ca2+ ions represent versatile second messengers that regulate a huge diversity of processes throughout the cell's life. One prominent Ca2+ entry pathway into the cell is the Ca2+ release-activated Ca2+ (CRAC) ion channel. It is fully reconstituted by the two molecular key players: the stromal interaction molecule (STIM1) and Orai. STIM1 is a Ca2+ sensor located in the membrane of the endoplasmic reticulum, and Orai, a highly Ca2+ selective ion channel embedded in the plasma membrane. Ca2+ store-depletion leads initially to the activation of STIM1 which subsequently activates Orai channels via direct binding. Authentic CRAC channel hallmarks and biophysical characteristics include high Ca2+ selectivity with a reversal potential in the range of + 50 mV, small unitary conductance, fast Ca2+-dependent inactivation and enhancements in currents upon the switch from a Na+-containing divalent-free to a Ca2+-containing solution. This review provides an overview on the critical determinants and structures within the STIM1 and Orai proteins that establish these prominent CRAC channel characteristics.

Authors+Show Affiliations

Institute of Biophysics, Johannes Kepler University of Linz, Gruberstrasse 40, 4020, Linz, Austria.Institute of Biophysics, Johannes Kepler University of Linz, Gruberstrasse 40, 4020, Linz, Austria.Institute of Biophysics, Johannes Kepler University of Linz, Gruberstrasse 40, 4020, Linz, Austria. isabella.derler@jku.at.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

30903264

Citation

Krizova, Adéla, et al. "Critical Parameters Maintaining Authentic CRAC Channel Hallmarks." European Biophysics Journal : EBJ, vol. 48, no. 5, 2019, pp. 425-445.
Krizova A, Maltan L, Derler I. Critical parameters maintaining authentic CRAC channel hallmarks. Eur Biophys J. 2019;48(5):425-445.
Krizova, A., Maltan, L., & Derler, I. (2019). Critical parameters maintaining authentic CRAC channel hallmarks. European Biophysics Journal : EBJ, 48(5), 425-445. https://doi.org/10.1007/s00249-019-01355-6
Krizova A, Maltan L, Derler I. Critical Parameters Maintaining Authentic CRAC Channel Hallmarks. Eur Biophys J. 2019;48(5):425-445. PubMed PMID: 30903264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Critical parameters maintaining authentic CRAC channel hallmarks. AU - Krizova,Adéla, AU - Maltan,Lena, AU - Derler,Isabella, Y1 - 2019/03/21/ PY - 2018/10/15/received PY - 2019/03/06/accepted PY - 2018/12/20/revised PY - 2019/3/25/pubmed PY - 2019/8/31/medline PY - 2019/3/24/entrez KW - CRAC channel KW - Calcium KW - Electrophysiology KW - FRET KW - Gain-of-function mutants KW - Orai gating KW - Orai1 KW - STIM1 KW - STIM–Orai interaction KW - Structural resolution SP - 425 EP - 445 JF - European biophysics journal : EBJ JO - Eur Biophys J VL - 48 IS - 5 N2 - Ca2+ ions represent versatile second messengers that regulate a huge diversity of processes throughout the cell's life. One prominent Ca2+ entry pathway into the cell is the Ca2+ release-activated Ca2+ (CRAC) ion channel. It is fully reconstituted by the two molecular key players: the stromal interaction molecule (STIM1) and Orai. STIM1 is a Ca2+ sensor located in the membrane of the endoplasmic reticulum, and Orai, a highly Ca2+ selective ion channel embedded in the plasma membrane. Ca2+ store-depletion leads initially to the activation of STIM1 which subsequently activates Orai channels via direct binding. Authentic CRAC channel hallmarks and biophysical characteristics include high Ca2+ selectivity with a reversal potential in the range of + 50 mV, small unitary conductance, fast Ca2+-dependent inactivation and enhancements in currents upon the switch from a Na+-containing divalent-free to a Ca2+-containing solution. This review provides an overview on the critical determinants and structures within the STIM1 and Orai proteins that establish these prominent CRAC channel characteristics. SN - 1432-1017 UR - https://www.unboundmedicine.com/medline/citation/30903264/Critical_parameters_maintaining_authentic_CRAC_channel_hallmarks_ L2 - https://dx.doi.org/10.1007/s00249-019-01355-6 DB - PRIME DP - Unbound Medicine ER -