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Apelin-36 exerts the cytoprotective effect against MPP+-induced cytotoxicity in SH-SY5Y cells through PI3K/Akt/mTOR autophagy pathway.
Life Sci. 2019 May 01; 224:95-108.LS

Abstract

AIMS

Parkinson's disease (PD) is a common neurodegenerative disease typically associated with the accumulation of α-synuclein. Autophagy impairment is thought to be involved in the dopaminergic neurodegeneration in PD. We investigate the effect of Apelin-36 on the activated phosphatidylinositol 3-kinase (PI3K)/protein kinase B(Akt)/the mammalian target of rapamycin (mTOR) autophagy pathway in 1-methyl-4-phenylpyridinium (MPP+)-treated SH-SY5Y cells, which is involved in the cytoprotective effect of Apelin-36.

MAIN METHODS

SH-SY5Y cells were treated with 1-Methyl-4-phenylpyridine (MPP+) with or without Apelin-36. The cell viability, apoptotic ratio, the form of autophagic vacuoles, the expression of tyrosine hydroxylase (TH), α-synuclein, phosphorylation of PI3K, AKT, mTOR, microtubule-associated protein 1 Light Chain 3 II/I (LC3II/I) and p62 were detected to investigate the neuroprotective effect of Apelin-36.

KEY FINDINGS

The results indicate that Apelin-36 significantly improved the cell viability and decreased the apoptosis in MPP+-treated SH-SY5Y cells. The decreased expression of tyrosine hydroxylase (TH) induced by MPP+ was significantly increased by Apelin36 pretreatment. Moreover, Apelin36 significantly increased the autophagic vacuoles. The ratio of LC3II/I was significantly increased by Apelin36, as well as the decreased p62 expression. In addition, the activated PI3K/AKT/mTOR pathway induced by MPP+ was significantly inhibited by Apelin36. Additionally, Apelin36 significantly decreased the α-synuclein expression. Furthermore, the cytoprotective effect of Apelin-36 was weakened by pretreatment with Insulin-like Growth Factor-1 (IGF-1), an activator of PI3K/Akt, and MHY1485, an mTOR activator.

SIGNIFICANCE

Our results demonstrated that Apelin-36 protects against MPP+-induced cytotoxicity through PI3K/Akt/mTOR autophagy pathway in PD model in vitro, which provides a new theoretical basis for the treatment of PD.

Authors+Show Affiliations

Cheeloo College of Medicine, Shandong University, 250014 Jinan, China.Neurobiology Institute, Jining Medical University, 272067 Jining, China.Neurobiology Institute, Jining Medical University, 272067 Jining, China.Neurobiology Institute, Jining Medical University, 272067 Jining, China.Neurobiology Institute, Jining Medical University, 272067 Jining, China.Neurobiology Institute, Jining Medical University, 272067 Jining, China. Electronic address: wangchunmei410@163.com.Neurobiology Institute, Jining Medical University, 272067 Jining, China. Electronic address: chengbh1979@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30905782

Citation

Zhu, Junge, et al. "Apelin-36 Exerts the Cytoprotective Effect Against MPP+-induced Cytotoxicity in SH-SY5Y Cells Through PI3K/Akt/mTOR Autophagy Pathway." Life Sciences, vol. 224, 2019, pp. 95-108.
Zhu J, Dou S, Jiang Y, et al. Apelin-36 exerts the cytoprotective effect against MPP+-induced cytotoxicity in SH-SY5Y cells through PI3K/Akt/mTOR autophagy pathway. Life Sci. 2019;224:95-108.
Zhu, J., Dou, S., Jiang, Y., Bai, B., Chen, J., Wang, C., & Cheng, B. (2019). Apelin-36 exerts the cytoprotective effect against MPP+-induced cytotoxicity in SH-SY5Y cells through PI3K/Akt/mTOR autophagy pathway. Life Sciences, 224, 95-108. https://doi.org/10.1016/j.lfs.2019.03.047
Zhu J, et al. Apelin-36 Exerts the Cytoprotective Effect Against MPP+-induced Cytotoxicity in SH-SY5Y Cells Through PI3K/Akt/mTOR Autophagy Pathway. Life Sci. 2019 May 1;224:95-108. PubMed PMID: 30905782.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apelin-36 exerts the cytoprotective effect against MPP+-induced cytotoxicity in SH-SY5Y cells through PI3K/Akt/mTOR autophagy pathway. AU - Zhu,Junge, AU - Dou,Shanshan, AU - Jiang,Yunlu, AU - Bai,Bo, AU - Chen,Jing, AU - Wang,Chunmei, AU - Cheng,Baohua, Y1 - 2019/03/21/ PY - 2018/12/12/received PY - 2019/03/08/revised PY - 2019/03/19/accepted PY - 2019/3/25/pubmed PY - 2019/5/21/medline PY - 2019/3/26/entrez KW - 1-Methyl-4-phenylpyridine KW - Apelin-36 KW - Apoptosis KW - Autophagy KW - Parkinson's disease SP - 95 EP - 108 JF - Life sciences JO - Life Sci VL - 224 N2 - AIMS: Parkinson's disease (PD) is a common neurodegenerative disease typically associated with the accumulation of α-synuclein. Autophagy impairment is thought to be involved in the dopaminergic neurodegeneration in PD. We investigate the effect of Apelin-36 on the activated phosphatidylinositol 3-kinase (PI3K)/protein kinase B(Akt)/the mammalian target of rapamycin (mTOR) autophagy pathway in 1-methyl-4-phenylpyridinium (MPP+)-treated SH-SY5Y cells, which is involved in the cytoprotective effect of Apelin-36. MAIN METHODS: SH-SY5Y cells were treated with 1-Methyl-4-phenylpyridine (MPP+) with or without Apelin-36. The cell viability, apoptotic ratio, the form of autophagic vacuoles, the expression of tyrosine hydroxylase (TH), α-synuclein, phosphorylation of PI3K, AKT, mTOR, microtubule-associated protein 1 Light Chain 3 II/I (LC3II/I) and p62 were detected to investigate the neuroprotective effect of Apelin-36. KEY FINDINGS: The results indicate that Apelin-36 significantly improved the cell viability and decreased the apoptosis in MPP+-treated SH-SY5Y cells. The decreased expression of tyrosine hydroxylase (TH) induced by MPP+ was significantly increased by Apelin36 pretreatment. Moreover, Apelin36 significantly increased the autophagic vacuoles. The ratio of LC3II/I was significantly increased by Apelin36, as well as the decreased p62 expression. In addition, the activated PI3K/AKT/mTOR pathway induced by MPP+ was significantly inhibited by Apelin36. Additionally, Apelin36 significantly decreased the α-synuclein expression. Furthermore, the cytoprotective effect of Apelin-36 was weakened by pretreatment with Insulin-like Growth Factor-1 (IGF-1), an activator of PI3K/Akt, and MHY1485, an mTOR activator. SIGNIFICANCE: Our results demonstrated that Apelin-36 protects against MPP+-induced cytotoxicity through PI3K/Akt/mTOR autophagy pathway in PD model in vitro, which provides a new theoretical basis for the treatment of PD. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/30905782/Apelin_36_exerts_the_cytoprotective_effect_against_MPP+_induced_cytotoxicity_in_SH_SY5Y_cells_through_PI3K/Akt/mTOR_autophagy_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(19)30211-5 DB - PRIME DP - Unbound Medicine ER -