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Enzyme-linked immunosorbent assay for human urokinase-type plasminogen activator and its proenzyme using a combination of monoclonal and polyclonal antibodies.
J Immunoassay. 1986; 7(3):209-28.JI

Abstract

A sensitive and specific enzyme-linked immunosorbent assay (ELISA) for human urokinase-type plasminogen activator (u-PA) and its inactive proenzyme (pro-u-PA) was developed. A monoclonal antibody was used as solid-phase antibody, while rabbit antibodies against human u-PA followed by peroxidase-conjugated third antibody were used for detection of bound u-PA. No reaction was observed with tissue-type plasminogen activator or with a variety of other human proteins. The assay was used for quantitation of u-PA in human urine and in culture fluid from human tumor cells. The recovery of added pro-u-PA was greater than 95%. A good agreement with the results obtained by enzymatic assays was found. The detection limit was less than 0.1 ng per ml, both for u-PA and pro-u-PA. The advantages of the use of ELISA compared with enzymatic assays and radioimmunoassays for quantitation of u-PA and pro-u-PA in biological samples are discussed.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3091639

Citation

Nielsen, L S., et al. "Enzyme-linked Immunosorbent Assay for Human Urokinase-type Plasminogen Activator and Its Proenzyme Using a Combination of Monoclonal and Polyclonal Antibodies." Journal of Immunoassay, vol. 7, no. 3, 1986, pp. 209-28.
Nielsen LS, Grøndahl-Hansen J, Andreasen PA, et al. Enzyme-linked immunosorbent assay for human urokinase-type plasminogen activator and its proenzyme using a combination of monoclonal and polyclonal antibodies. J Immunoassay. 1986;7(3):209-28.
Nielsen, L. S., Grøndahl-Hansen, J., Andreasen, P. A., Skriver, L., Zeuthen, J., & Danø, K. (1986). Enzyme-linked immunosorbent assay for human urokinase-type plasminogen activator and its proenzyme using a combination of monoclonal and polyclonal antibodies. Journal of Immunoassay, 7(3), 209-28.
Nielsen LS, et al. Enzyme-linked Immunosorbent Assay for Human Urokinase-type Plasminogen Activator and Its Proenzyme Using a Combination of Monoclonal and Polyclonal Antibodies. J Immunoassay. 1986;7(3):209-28. PubMed PMID: 3091639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme-linked immunosorbent assay for human urokinase-type plasminogen activator and its proenzyme using a combination of monoclonal and polyclonal antibodies. AU - Nielsen,L S, AU - Grøndahl-Hansen,J, AU - Andreasen,P A, AU - Skriver,L, AU - Zeuthen,J, AU - Danø,K, PY - 1986/1/1/pubmed PY - 1986/1/1/medline PY - 1986/1/1/entrez SP - 209 EP - 28 JF - Journal of immunoassay JO - J Immunoassay VL - 7 IS - 3 N2 - A sensitive and specific enzyme-linked immunosorbent assay (ELISA) for human urokinase-type plasminogen activator (u-PA) and its inactive proenzyme (pro-u-PA) was developed. A monoclonal antibody was used as solid-phase antibody, while rabbit antibodies against human u-PA followed by peroxidase-conjugated third antibody were used for detection of bound u-PA. No reaction was observed with tissue-type plasminogen activator or with a variety of other human proteins. The assay was used for quantitation of u-PA in human urine and in culture fluid from human tumor cells. The recovery of added pro-u-PA was greater than 95%. A good agreement with the results obtained by enzymatic assays was found. The detection limit was less than 0.1 ng per ml, both for u-PA and pro-u-PA. The advantages of the use of ELISA compared with enzymatic assays and radioimmunoassays for quantitation of u-PA and pro-u-PA in biological samples are discussed. SN - 0197-1522 UR - https://www.unboundmedicine.com/medline/citation/3091639/Enzyme_linked_immunosorbent_assay_for_human_urokinase_type_plasminogen_activator_and_its_proenzyme_using_a_combination_of_monoclonal_and_polyclonal_antibodies_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:3091639 DB - PRIME DP - Unbound Medicine ER -